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Cotton high-strong fiber gene major gene site and moloecular labelling thereof

A technology of main effect genes and high-strength fibers, applied in the field of discovery and utilization of new genetic resources, can solve the problems of high cost, difficulty, and slow progress in quality breeding, and achieve the goals of improving fiber quality, accelerating industrialization, and improving selection efficiency Effect

Inactive Publication Date: 2004-09-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Therefore, the cost of quality breeding is high, difficult and slow

Method used

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  • Cotton high-strong fiber gene major gene site and moloecular labelling thereof
  • Cotton high-strong fiber gene major gene site and moloecular labelling thereof

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Experimental program
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Embodiment Construction

[0031] The implementation procedure of the present invention is: the upland cotton high-strength fiber germplasm line HS427-10 introduced from the Crop Germplasm Resources Laboratory of the Southern Plains Research Center of the United States Department of Agriculture is selected for individual plants, and the fiber quality is measured, and the homozygous and stable ones are selected. Strain HS427-10, according to the measurement results in 2000 and 2001, the average fiber quality of strain HS427-10 in two years is: fiber strength 42.3g / tex, fiber length 32.6mm, fiber fineness 5.18, upland cotton genetics The standard line TM-1 (American Daizi cotton 14 varieties self-crossed for more than 15 generations, provided by Dr. Kohel of the Southern Plains Agricultural Research Center of the Department of Agriculture, University City, Texas, USA) is: fiber strength 31.33g / tex, fiber length 30.7mm, fiber fineness 5.39.

[0032] In 1999, the strain HS427-10 was crossed with TM-1, and a...

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Abstract

The invention discloses major gene resistance locus and the molecular mark of cotton high intensity fiber, which is used auxiliary selection of molecular mark of cotton fiber intensity quickly. The major gene resistance locus QTLfs-2 has three SSR marks H H which connect with it, they are located by mark NAU / SSR / FS1130, NAU / SSR / FS2160, NAU / SSR / FS3160, and it can explain 16.9% phenotype variation in F2:3 of HS427-10xTM-1. Most of high intensity fibers have the same gene QTLfs-2, it has high application value. The expression is stable. It can enhance the fiber quality of cotton in our country and accelerates the industrialization of high intensity cotton seeds.

Description

(1) Technical field [0001] The invention discloses a main effect gene site of a cotton high-strength fiber gene and a molecular marker thereof, and belongs to a method for excavating and utilizing new gene resources. It is specially used for molecular marker-assisted selection of rapid cotton fiber specific strength, and carries out molecular breeding, production and quality inspection of cotton fiber quality. (2) Technical background [0002] Cotton fiber is an important raw material for the textile industry. With the continuous innovation of textile technology to reduce costs and improve textile efficiency, air rotor spinning, air-jet spinning and friction spinning technologies are rapidly eliminating old-fashioned ring spinning technologies. These new textile technologies cannot To make full use of the inherent quality potential of cotton fiber, there are higher requirements for fiber quality. Therefore, there is an urgent need to rapidly improve the quality of cotton fi...

Claims

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Application Information

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IPC IPC(8): C12P19/34C12Q1/68
Inventor 张天真沈新莲郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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