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SNP (single-nucleotide polymorphism) marker set for identifying pig breeds and application thereof

A marker and genome-wide technology, applied in the direction of recombinant DNA technology, microbial determination/testing, DNA/RNA fragments, etc.

Inactive Publication Date: 2016-05-25
ZHANGZHOU AONONG MODERN AGRI DEV CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But so far, there are few SNP marker combinations for variety identification and individual identification

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  • SNP (single-nucleotide polymorphism) marker set for identifying pig breeds and application thereof

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Embodiment Construction

[0013] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

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Abstract

The invention discloses an SNP (single-nucleotide polymorphism) marker set for identifying pig breeds. The application method mainly comprises the following steps: by using 200 purebred Large White and Landrace individuals as study objects, carrying out genome SNP 60K chip scanning typing on all the samples of the two breeds; after carrying out quality control on all the data according to the requirements, screening out SNP marker sites capable of identifying Landrace and Large White, and carrying out genome correlation analysis to screen out 16 SNP sites for identifying Landrace and Large White; and carrying out verification on another 500 individuals by principal component analysis and cluster analysis, wherein the accuracy of the 16 SNP sites reaches 99%, which indicates that the 16 SNP sites can be used for identifying Large White and Landrace.

Description

technical field [0001] The invention relates to the technical field of SNP markers, in particular to a group of SNP markers for identifying pig breeds and applications thereof. Background technique [0002] China is one of the earliest countries in the world to raise pigs, with a history of thousands of years. Pigs are known as the first of the six animals in China. Due to the influence of natural environment, economic conditions and folk culture, China's local pig breeds are rich in resources and have their own characteristics, which are valuable resources in the world's pig breed gene pool. But so far, there are few SNP marker combinations for variety identification and individual identification. [0003] With the application of high-density SNP chips in pigs, the popularization and development of whole genome sequencing technology, and the continuous decline in genotyping and sequencing costs, it is possible to establish individual tracking technology and breed identific...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 龙毅温庆琪肖俊峰吴德丁能水
Owner ZHANGZHOU AONONG MODERN AGRI DEV CO LTD
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