Upland cotton SNP marker and application thereof

An upland cotton, marker technology, applied in recombinant DNA technology, DNA/RNA fragments, chemical libraries, etc., can solve the problem of failure to analyze linkage disequilibrium recession distance, etc., and achieve the effect of improving detection efficiency and high work efficiency.

Inactive Publication Date: 2016-02-24
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In rice, a SNP chip (Chinese patent CN104328507A) has been developed for rice variety identification. However, in the development process of this invention, although the linkage disequilibrium (LD) between SNP markers was considered, it did not analyze the specific The chain disequilibrium recession distance

Method used

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  • Upland cotton SNP marker and application thereof
  • Upland cotton SNP marker and application thereof
  • Upland cotton SNP marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Development and Application of Upland Cotton SNP Markers

[0024] 1. Experimental method

[0025] 1.1 Test materials

[0026] Total DNA was extracted from tender green leaves of 120 excellent germplasm materials of upland cotton (Table 1).

[0027] Table 1 Cotton strains and materials whose total DNA was extracted from tender green leaves for analysis

[0028]

[0029]

[0030]

[0031]

[0032] 1.2 Total DNA extraction method

[0033] Utilize DNA extraction kit (TaKaRaMiniBESTPlantGenomicDNAExtractionKit, CodeNo.9768) to extract the DNA of each material seedling, DNA quality requirement is: DNA concentration is greater than 50ng / ul; DNA sample volume is greater than 20ul, DNA total amount is greater than 1ug, and the absorbance ratio of DNA 260 / 280 Between 1.7-2.0.

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Abstract

The invention discloses an upland cotton SNP marker and application thereof, and belongs to the field of upland cotton SNP marker development. The upland cotton SNP marker is obtained by developing a single-copy SNP marker of an upland cotton genome via chip hybridization and homologous sequence comparison, and picking out SNP markers in a linkage disequilibrium recession distance via linkage disequilibrium analysis. The SNP marker provided by the invention can be applied to analysis of genetic diversity and group structure, or to germplasm identification of upland cotton, is single-copy, and has no remarkable linkage disequilibrium among markers, so that the efficiency of detection, adopting the SNP marker, during analysis of genetic diversity, group structure or fingerprint identification can be greatly improved, and the work efficiency is higher.

Description

technical field [0001] The invention relates to SNP markers, in particular to SNP markers of upland cotton, and also relates to the application of the upland cotton SNP markers in the analysis of genetic diversity and identification of germplasm resources of upland cotton, belonging to the development field of upland cotton SNP markers. Background technique [0002] Single nucleotide polymorphism (SNPs for short) markers have the characteristics of large number and high distribution density in the genome, and in the genotyping process, there is no need to judge according to the fragment size, and it can be completed automatically on a large scale. Compared with repeat sequence polymorphism (SSR) markers such as microsatellite markers, SNPs have high genetic stability, especially SNPs in coding regions, which have higher work efficiency and are more suitable for large-scale detection and analysis, so Biologists now believe that SNP markers are a promising molecular marker tec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C40B40/06
Inventor 赵云雷王红梅陈伟赵佩龚海燕桑晓慧崔艳利
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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