SNP (Single Nucleotide Polymorphism) marker, kit and method for rapidly identifying panax traditional Chinese medicines including panax stipuleanatus and panax quinquefolius

A kit and ginseng technology are applied in the field of SNP labeling and kits to achieve the effects of rapid identification, high specificity and high amplification efficiency

Active Publication Date: 2017-04-26
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for precise comparative analysis between different types of plant material such as people's food or medicine products without being limited only to their appearance features like coloration or taste. It also provides accurate results even when analyzed from samples collected on non-natural surfaces (such as rocks).

Problems solved by technology

This patented describes different techniques that can be applied to determine if or how much certain ingredients like peppermint oil should contain more beta hydroxypropyltripeptides called quinolone diphosphate glucosyl transferase (QDPG). These compounds help protect against damage from excessive alcohol consumption during pregnancy while still having good effects on their body's healthy organs.

Method used

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  • SNP (Single Nucleotide Polymorphism) marker, kit and method for rapidly identifying panax traditional Chinese medicines including panax stipuleanatus and panax quinquefolius
  • SNP (Single Nucleotide Polymorphism) marker, kit and method for rapidly identifying panax traditional Chinese medicines including panax stipuleanatus and panax quinquefolius
  • SNP (Single Nucleotide Polymorphism) marker, kit and method for rapidly identifying panax traditional Chinese medicines including panax stipuleanatus and panax quinquefolius

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: identification method of Pingbian Radix Notoginseng raw material

[0033] The samples of Panax genus were collected from different producing areas, and the specific medicinal materials and collection locations represented by each sample are shown in Table 1. 25mg samples were taken and ground on MM400 ball mill (Retsch, Germany), and the total DNA was extracted with the plant genomic DNA kit of Tiangen Biochemical Technology (Beijing) Co., Ltd.

[0034] Table 1: Panax samples

[0035] serial number Sample serial number herbs sampling location 1 RS1-6 ginseng Fusong County, Jilin Province 2 BB1-6 Bamboo ginseng Hubei Enshi 3 R1-3 Bamboo ginseng Japan 4 ZZ1-6 Ginseng Gansu 5 XZ1-6 Ginseng Nyingchi, Tibet 6 JZ1-5 Ginger notoginseng Myanmar 7 XY1-2 Panax ginseng Mount Emei, Sichuan 8 PB1-13 Panax notoginseng Yunnan

[0036] PCR amplification

[0037] The primer...

Embodiment 2

[0050] Embodiment 2: identification method of Pingbian Sanqi decoction pieces

[0051] Compared with Example 1, the only difference is that the Pingbian notoginseng decoction pieces are used as samples, DNA is extracted and identified, and the above two SNP sites can also be specifically detected. During the sequence comparison, it was found that the 88th base from the 5' end of the sequence of SEQ ID NO.1 of Pingbian Sanqi Decoction Pieces is A, and the 117th base is C.

Embodiment 3

[0052] Embodiment 3: Identification of Pingbian Panax notoginseng powder

[0053] Compared with Example 1, the only difference is that the Pingbian notoginseng powder is used as a sample, DNA is extracted and identified, and the above two SNP sites can also be specifically detected as a result. During the sequence comparison, it was found that the 88th base from the 5' end of the sequence of SEQ ID NO.1 of Pingbian Sanqi Decoction Pieces is A, and the 117th base is C.

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Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for rapidly identifying panax stipuleanatus and counterfeit species thereof. The nucleotide sequence of the SNP marker is shown as SEQ ID No.1; an 88th site from a 5 end of the sequence is A and/or a 117th site is C. The invention further relates to a kit for rapidly identifying the panax stipuleanatus and the counterfeit species thereof, and the kit comprises a primer which is used for amplifying to obtain the sequence shown as SEQ ID No.1. The invention further relates to a method for rapidly identifying the panax stipuleanatus and the counterfeit species thereof; the method comprises the following steps: taking a genome DNA (Deoxyribonucleic Acid) of a sample to be identified as a template and amplifying the sequence shown as SEQ ID No.1; detecting an 88th site basic group and a 117th site basic group from the 5 end in an obtained amplified fragment, so as to judge that the sample to be identified is the panax stipuleanatus or the counterfeit species thereof. By adopting the method provided by the invention, the panax stipuleanatus and the counterfeit species with similar morphological characteristics can be accurately distinguished; the method has wide applicability and can be used for detecting any sample of the panax stipuleanatus capable of being used for extracting the DNAs, and rapid and accurate identification of the panax stipuleanatus can be realized.

Description

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Claims

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Application Information

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Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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