Molecular marker capable of influencing intramuscular fat content of Duroc boar and application

A technology for intramuscular fat and molecular markers, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems of limiting the application of molecular markers for complex traits and large confidence regions, and achieve the goal of increasing core competitiveness, improving economic benefits, and improving meat quality Effect

Active Publication Date: 2018-07-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the candidate gene method has the advantages of simple method and convenient operation, it can only target genes with known biological functions; while the confidence region of the candidate gene identified by the QTL mapping method is also large, which greatly limits the ability of complex trait molecules Application of markers in livestock genetics and breeding

Method used

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  • Molecular marker capable of influencing intramuscular fat content of Duroc boar and application
  • Molecular marker capable of influencing intramuscular fat content of Duroc boar and application
  • Molecular marker capable of influencing intramuscular fat content of Duroc boar and application

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Embodiment 1

[0035] 1. Experimental animals

[0036] The group of experimental pigs used in the present invention is 2296 purebred American Duroc of the Breeding Pig Branch of Guangdong Wens Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.

[0037] In this experiment, Chinese and American Duroc pigs were selected from this resource group. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0038] 2. Sample collection

[0039] The above-mentioned piglet docked tail and ear tissues were collected, soaked in 75% ethanol, and stored in a -20°C refrigerator for later use.

[0040] 3. 50K SNP typing in the whole pig genome

[0041] From the ear tissue or docked tail tissue collected from each individual of the 2296 Duroc species selected from the above resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method, and each piece was accu...

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Abstract

The invention belongs to the fields of molecular biological technology and molecular marker technology and relates to an SNP molecular marker related to intramuscular fat content of a Duroc boar. Thesite of the SNP marker is the 117427086th nucleotide site on the chromosome 7 of the International Pig Reference Genome 11.1 version, and the basic group of the site is G or A. By preferably selectingthe dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the intramuscular fat content of the boar can be increased, and the genetic improvementprogress of the boar can be accelerated, thereby effectively improving the economic benefit of boar breeding.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and relates to a SNP molecular marker related to intramuscular fat content of Duroc pigs and its application. Background technique [0002] With the improvement of people's living standards, the quality of pork has received more and more attention. Among the many factors affecting pork quality, Intramuscular Fat (IMF) is an important indicator, which affects the tenderness, water retention, shear force value, flavor and juiciness of meat. An appropriate amount of intramuscular fat can make the meat tender, delicious, juicy, and therefore, the research on the factors that affect the intramuscular fat content of pigs has always been the focus of breeders. [0003] In the past hundred years, most of the lean-meat breeding pigs represented by Duroc have focused on increasing lean meat percentage and reducing backfat thickness as important breeding goals. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨杰吴珍芳庄站伟丁荣荣郑恩琴蔡更元
Owner SOUTH CHINA AGRI UNIV
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