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SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof

A technology of mouse inbred strains and primer pairs, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that cannot be fully adapted to the genetic monitoring work of various strains of experimental animals, and achieve Promote the standardization process, reduce the cost of testing, and promote the effect of standardization and process

Inactive Publication Date: 2015-10-14
SOUTH CHINA UNIV OF TECH +1
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AI Technical Summary

Problems solved by technology

These methods have their own advantages, and they are still of vital value in the breeding and daily monitoring and management of experimental animals. However, with the rapid development of experimental animal science and molecular biology, traditional detection methods including national standards The current biochemical marker gene detection method, skin transplantation method and immunological detection method can no longer fully adapt to the genetic monitoring work of various strains of experimental animals

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  • SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof
  • SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof
  • SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof

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Embodiment 1

[0032] 1. Experimental animal samples

[0033] Mouse inbred strains: 129, C3H, BALB, C57BL, CBA, DBA, FVB, ICR.

[0034] 2. Sources of animal tissues: Guangdong Laboratory Animal Monitoring Institute, Jinan University and Southern Medical University, etc.

[0035] 3. Screening of target SNP

[0036] Take advantage of https: / / www.ncbi.nlm.nih.gov / snp / ? The term=mouse database combined with relevant literature reports screened out 7 SNP sites. See Table 1 for details.

[0037] Table 1 SNP markers and their genotypes in each mouse inbred strain

[0038] SNP IDs

Chr

SEQ ID

Location

129

C3H

BALB / cJ

C57BL / 6

C57BL / 10

CBA / C

CBA / J

DBA / 1

FVB

ICR

SNP1

7

NO:15

91

G

G

G

T

T

G

G

G

G

G

SNP2

11

NO:16

196

A

C

C

A

C

A

A

A

A

C

SNP3

15

NO:17

112

A

A

G

G

G

A

A

A

...

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Abstract

The invention discloses SNP (single-nucleotide polymorphism) markers and primer pairs for mouse inbred line identification, and application thereof. 7 screened SNP sites, including SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7, are utilized to design 7 primer pairs of which the sequences are disclosed as SEQ ID NO:1-SEQ ID NO:14; and each primer pair can amplify genome sequences disclosed as SEQ ID NO:15-SEQ ID NO:21 of the mouse inbred line under the same conditions. The sequences amplified by each primer pair contain one specific SNP marker. The application of the SNP marker and primer pairs can achieve the goals of quickly performing primary screening and lowering the detection cost, thereby promoting the inbred line mouse genetic quality monitoring standardization progress.

Description

technical field [0001] The invention relates to the use of the advantages of whole genome biological information analysis technology, and the use of PCR amplification and sanger sequencing to verify SNP markers, and the application of mass spectrometry and SNP markers to detect and identify mouse inbred strains in production units, especially for use in mice SNP markers, primer pairs and their applications for inbred identification. Background technique [0002] At present, the domestic biomedical industry is developing rapidly. As an important supporting condition for the biomedical industry and an important material for life science research, experimental animals are directly related to the reliability and authenticity of experimental results. The genetic quality of experimental animals is one of the important factors for the quality of experimental animals. Only the use of experimental animals with stable genetic characteristics can ensure the comparability of biomedical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 杜红丽关宇佳李舸张钰张燕宇
Owner SOUTH CHINA UNIV OF TECH
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