4 results about "Gleditsia triacanthos" patented technology

The invention belongs to the technical field of plant germplasm resource preservation, and provides a special protective agent for pollen integrated treatment of Chinese honey locust hybrid flowers, a kit and an integrated treatment method thereof. The protective agent is prepared from 8%-12% w / v of trehalose, 0.05%-0.08% v / v of Triton X-100 and a phosphate buffer solution with the pH value of 7.0. The integrated treatment method comprises the following steps: performing stamen morphology primary screening and separation on the Chinese honey locust flowers at low temperature; grinding in an ice bath by using a protective agent, filtering, centrifuging and enriching fertile pollen; quickly evaluating the activity of the split-flow part suspension by using an MTT method; carrying out trehalose-tert-butyl alcohol gradient dehydration and low-temperature vacuum drying on the residual part to obtain dried pollen, and storing for a long time; and treating with rehydration liquid before use. The extraction damage is reduced to the maximum extent; tert-butyl alcohol and trehalose synergistically dehydrate and dry, so that the pollen safely enters a vitrification state, and long-term stable preservation is realized; the repeatability is high, standardized operation is achieved, and a reliable tool is provided for gleditsia sinensis germplasm resource preservation and breeding.

The application discloses a Dai medicine composition for treating eye diseases and a preparation and application thereof. The Dai medicine composition for treating eye diseases is composed of chrysanthemum, carthamus tinctorius seed, periostracum cicadae, asarum, imperata cylindrica, Daemonorops draco, honey locust, and licorice. The Dai medicine composition for treating eye diseases is added with pharmaceutically acceptable adjuvants to be prepared into a preparation. The Dai medicine composition for treating eye diseases and the preparation thereof can be used for preparing a medicine for treating eye diseases.

The invention discloses a preparation method and a quality detection method of a honey-fried pagodatree pod standard decoction, and relates to the technical field of quality research of traditional Chinese medicinal materials. The method comprises the following steps: weighing a proper amount of honey sophora fruit decoction pieces, putting the decoction pieces into a marmite, decocting twice, adding 8 times of water for soaking for 30 minutes for the first time, boiling with strong fire, and then decocting with slow fire for 30 minutes; adding 6 times of water for the second time, boiling with strong fire, then decocting with slow fire for 20 minutes, filtering the liquid medicine with 200 meshes, cooling the filtrate to room temperature in a cold water bath, combining the two filtrates, carrying out vacuum concentration at the temperature of 50 DEG C, carrying out vacuum concentration at the absolute vacuum degree of 30-40mbar until the weight ratio of the medicinal material to the fluid extract is about 1: 1, collecting the concentrated solution, and carrying out freeze drying to obtain a sample. According to the method, key quality indexes such as the main content, the characteristic chromatogram and the extract of the honey-fried pagodatree pods after the decoction is prepared are measured, the transfer rate is investigated, the component transfer condition from decoction pieces to the decoction is measured through objective quality standards, and the quality degree and effectiveness of the decoction are ensured.

The invention provides a traditional Chinese medicine preparation for treating or improving cognitive impairment, and particularly relates to the technical field of traditional Chinese medicine pharmacy. The traditional Chinese medicine preparation is prepared from the following raw materials: rhizoma atractylodis, radix pseudostellariae, flatstem milkvetch seed, oviductus ranae, trogopterus dung, cynanchum glaucescens, amber, Japanese ardisia herb, Chinese honey locust, calculus bovis factitius, armeniaca mume, trichosanthes kirilowii maxim and maltose. In the traditional Chinese medicine preparation, oviductus ranae and calculus bovis factitius are used as monarch drugs, flatstem milkvetch seeds, Chinese honey locust, rhizoma atractylodis, trogopterus dung and amber are used as ministerial drugs, radix pseudostellariae, cynanchum glaucescens, Japanese ardisia herb, trichosanthes kirilowii maxim, armeniaca mume and maltose are used as adjuvant drugs, and the whole formula has the effects of tonifying kidney and filling marrow to treat root causes, eliminating phlegm and inducing resuscitation, promoting blood circulation to remove blood stasis, clearing heat and reducing phlegm to treat symptoms, tonifying spleen and replenishing qi, and soothing liver and regulating qi; all the components are used together, so that the effects of replenishing marrow, nourishing brain, inducing resuscitation, promoting intelligence and improving cognition are achieved. The traditional Chinese medicine preparation is expected to be further developed and applied as a new medicine for treating or improving cognitive impairment.