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Method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis

A technology of capillary electrophoresis and root mushroom, which is applied in the field of chemistry, can solve the problems of few research and application reports on the identification of oospore root mushroom fruiting phenotypes, and achieve the effects of being friendly to people and the environment, high in feasibility, and reducing pollution

Pending Publication Date: 2022-02-11
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the research and application of capillary electrophoresis to identify the fruiting phenotype of Rhizoma oospore

Method used

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  • Method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis
  • Method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis
  • Method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis

Examples

Experimental program
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Embodiment 1

[0031] A method for identifying the fruiting phenotype of Rhizoma oospore based on high-efficiency capillary electrophoresis, comprising the steps of:

[0032] (1) Sample solution treatment: inoculate in PDA liquid medium after activation of oospore rhizome mushroom (place of origin Fujian, fruiting body shape nearly columnar, fruiting body dark brown, with fluff, gills, bacterial flesh all white), 22°C, 160r / min shaker culture 7-9d. After filtering the double-layer gauze, wash the mycelium with double-distilled water for 2-3 times, dry and pulverize the obtained bacteria powder, add ultra-pure water at a ratio of 1:15, and ultrasonicate for 30 minutes. Add 15 times of ultrapure water to ultrasonic extraction for 30 minutes, and combine the two supernatants. Use a 0.22μm pore size water filter membrane before use.

[0033] (2) Electrophoresis conditions: the effective detection length of uncoated quartz capillary is 48cm; the detection wavelength is 225nm; the separation vol...

Embodiment 2

[0035] The difference from Example 1 is: Liaoning, the place of production of the oospore rhizome mushroom, the fruiting body shape is nearly columnar, the fruiting body is dark brown, and has fluff, and the gills and bacterial flesh are all white; the fingerprints are as follows: figure 1 Shown in H2.

Embodiment 3

[0037] The difference from Example 1 is: Liaoning, the place of production of oospore root mushroom, the fruiting body shape is nearly spherical, the fruiting body is light brown, without fluff, and it is nearly smooth, and the gills and bacterial flesh are all white; the fingerprints are as follows: figure 1 Shown in H3.

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Abstract

The invention provides a method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis, the method comprises the following steps: carrying out capillary electrophoresis on a sample solution under the electrophoresis conditions that the effective detection length of an uncoated quartz capillary is 48-60cm; the detection wavelength is 190-225 nm; the separation voltage ranges from 25-30 kV; the temperature is 20-25 DEG C; the pressure is 0.5-0.8 psi, the sample introduction is performed for 3-5 seconds, and a running buffer solution is a borax buffer solution with the concentration of 25-50 mmol / L and a sodium citrate buffer solution with the concentration of 20-25 mmol / L; the operation time is 30-35 minutes, and obtaining the fingerprint spectrum. According to the method for identifying the fruiting phenotype of the Ooospora radicata based on efficient capillary electrophoresis, a W-shaped triple difference peak serves as a key indication peak for identifying the fruiting phenotype of different sporocarp of the Ooospora radicata, and a new method is provided for screening and identifying fruiting strains after wild domestication and cross breeding of the Ooospora radicata in the later period.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a method for distinguishing the fruiting phenotype of oospore root mushroom based on high-efficiency capillary electrophoresis. Background technique [0002] In the prior art, liquid chromatography is commonly used in the determination of edible fungus fingerprints, but this method requires frequent maintenance of chromatographic columns, high cost, and the use of organic solvents throughout the process. Solvent volatilization during the determination process will affect The health of the operator and the organic waste liquid after the measurement also need special treatment, but the discharge process will also cause pollution. The capillary electrophoresis method for identifying and measuring the fruiting phenotypes of oospore rhizomes provided by the invention does not require organic solvents during the operation of the capillaries, and the buffer is an inorganic salt solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 龚娜刘国丽马晓颖陈珣杨光马跃肖军肇莹王岩赵宸
Owner LIAONING ACAD OF AGRI SCI
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