Oudemansiella radicata protein extract and anti-tumor application thereof
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A technology of protein extract and rhizome mushroom, applied in the direction of antineoplastic drugs, peptide/protein components, applications, etc.
Active Publication Date: 2013-09-11
北京济全生物科技有限公司
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[0003] At present, the research on Rhizoma Rhizome mainly focuses on fermentation, cultivation and nutritional analysis. There are few reports on the pharmacological activity of Rhizoma Rhizome, and it is still blank to extract protein substances from fruiting bodies of Rhizome Rhizoma in vitro.
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Embodiment 1
[0032] Embodiment 1, the preparation of root mushroom protein extract
[0033] 1. Preparation of the fruiting body of Rhizome Mushroom
[0034] Inoculate the slant strain of Oudemansiella radicata CFCC89567 into the primary medium for activation, culture at a constant temperature of 25°C, and inoculate it into the secondary medium after the mycelium is full of the test tube, and cultivate at a constant temperature of 25°C Cultivate in the room until the hyphae are overgrown, inoculate the second-grade species into the cultivation bag with the cultivation medium for germination at 25°C, scratch the fungus after the strain is full of the cultivation bag, and move it into the greenhouse. Keep the humidity above 90% and the temperature at 17-28°C, collect the first tide fruiting body, and obtain the fruiting body of Oudemansiella radicata CFCC89567.
[0035] Wherein, the culture medium used in this experiment is as follows:
[0043] Example 2, Rhizome Rhizome Protein Extract Inhibits Tumor Cell Proliferation Experiment
[0044] 1.1 Tested cell lines
[0045] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).
[0046] 1.2 Experimental method
[0047] 1.2.1 Cell lines and cell culture
[0048] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%, and the volume percent...
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Abstract
The invention discloses an oudemansiella radicata protein extract and anti-tumor application thereof. The oudemansiella radicata protein extract is prepared according to the method comprising the following steps of: 1) extracting by water after crushing oudemansiella radicata sporocarp, collecting a water-soluble matter to obtain an oudemansiella radicata water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the oudemansiella radicata water-soluble extract, collecting sediments, and obtaining the oudemansiella radicata protein extract after dialyzing the sediments by deionized water. IC50 values are 5 mug / mL, 7 mug / mL, and 8 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the oudemansiella radicata protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.
Description
technical field [0001] The invention relates to the protein extract of rhizome mushroom and its anti-tumor application. Background technique [0002] Oudemansiella radicata, also known as Oudemansiella radicata, belongs to Basidiomycota, Phytomycetes, Agaricaceae, Oudemansiellaceae, Aureus. The mushroom tastes delicious, the stalk is crisp and delicious, rich in polysaccharides, fatty acids, proteins, amino acids, mineral elements, etc., and has high edible and medicinal value. Much research. Lin Yong conducted a nutritional evaluation of the protein in the long-rooted mushroom, and the results showed that the chemical score, amino acid score, biological value, and nutritional index of the fruiting body of the long-rooted mushroom were higher than those of the reference Dacup umbrella and tiger milk mushroom, indicating that the long-rooted mushroom Rhizome mushroom is a kind of mushroom with development prospect. Huang Wen explored the new technology of long-root mushroo...
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