Liquid deep layer fermenting process for producing extracellular polysaccharide using medicinal fungus long root mushroom

Abstract

The deep fermentation process uses the spawn of Oudemansiella radicata from Central-China Agricultural Univ. and includes the following steps: slant spawn culture, shake flask seed culture, 7L fermenting tank culture, and polysaccharide extraction and determination. The present invention selects corn powder and soybean cake powder as main fermentation material and has low production cost. It is found that palmitic acid is key nutritious additive factor capable of raising the yield of extracellular polysaccharide of Oudemansiella radicata up to 30-40 %. The fermentation process is controlled through on-line control of variation in dissolved oxygen concentration. The present invention can realize large scale fermentation with simple technological operation, and is suitable for industrial production.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, and is a technique for preparing known or new organisms or their metabolites by cultivating and fermenting organisms. In particular, it relates to a liquid submerged fermentation process for producing exopolysaccharides by using the medicinal fungus Rhizome pilosula. Background technique [0002] Long-rooted mushroom (Oudemansiella radicata), also known as Oudemansiella radicata, long-rooted small Oudemansiella, big hair grass fungus, long-rooted money fungus, etc. Belongs to Basidiomycotina, Phytomycetes, Agaricales, Oleumaceae, and Ouderia genus. Long root mushroom is the top grade edible, with tender inner quality, crisp and delicious handle, rich in protein, amino acid, fat, carbohydrates, vitamins and trace elements, with high edible value, and is a rare edible and medicinal fungus. In 1977, the Japanese scholar Shigebe Oto and others confirmed that the polysaccharides of rhizome m...

AI Technical Summary

Problems solved by technology

[0003] Long root mushroom is a new type of medicinal fungus introduced and cultivated in China in recent years. It is currently in the cultivation test and promotion stage. Due to the shortcomings of solid cultivation, such as long cycle, low yield, and climate impact, its large-scale development and use are limited, and liquid cultivation is used. Submerged fermentation culture technology is currently a new production method for in-depth development of active polysaccharide resources of medicinal fungi, and has successfully achieved large-scale production in rare medicinal fungi such as Ganoderma lucidum, Lentinus edodes, Grifola frondosa, and Agaricus blazei. The research on liquid submerged fermentation technology started relatively late, especially in the engineering research on the production of active polysaccharides by fermenting rhizome mushrooms on the reactor, and there are no relevant reports yet.