Pleurotus djamor protein extract and applications thereof for resisting tumor
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A technology of protein extract and oyster mushroom, applied in the direction of antineoplastic drugs, peptide/protein components, applications, etc.
Inactive Publication Date: 2013-09-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Extraction of protein from Pleurotus eryngii and research on its activity is still blank
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Embodiment 1
[0031] The preparation of embodiment 1, red oyster mushroom protein extract
[0032] 1. Preparation of red oyster mushroom fruiting bodies
[0033] Inoculate Pleurotus djamor (Pleurotus djamor) (agricultural) CFCC89574 slant bacteria into the first-level seed medium for activation, and culture at a constant temperature of 25°C. Cultivate in a constant temperature culture room at ℃ until the hyphae are overgrown, inoculate the secondary species into a cultivation bag with a cultivation medium at 25°C for germination, and then scratch the fungus after the strain is overgrown in the cultivation bag and move it into the greenhouse. The fruiting conditions are to keep the humidity above 90% and the temperature at 17-28°C. Collect the fruiting body of the first tide to obtain the fruiting body of Pleurotus djamor (agriculture) CFCC89574.
[0034] Wherein, the culture medium used in this experiment is as follows:
[0042] Embodiment 2, red oyster mushroom protein extract inhibits tumor cell proliferation experiment
[0043] 1.1 Tested cell lines
[0044] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).
[0045] 1.2 Experimental method
[0046] 1.2.1 Cell lines and cell culture
[0047] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%, and the volume ...
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Abstract
The invention discloses a pleurotus djamor protein extract and applications thereof for resisting tumor. The pleurotus djamor protein extract is prepared by adopting the method comprising the following steps of: 1) grinding pleurotus djamor fruiting body, then leaching with water, and collecting water-soluble matters to obtain water-soluble pleurotus djamor extract; and 2) carrying out 80% saturation of ammonium sulfate precipitation to the water-soluble pleurotus djamor extract, collecting the precipitate, and dialyzing the precipitate with deionized water to obtain the pleurotus djamor protein extract. After the pleurotus djamor protein extract is used for treating human liver cancer HepG2 cell, human breast cancer MCF-7 cell and human lung adenocarcinoma cell A-549 for 72 hours, the IC50 (50% inhibiting concentration) values are respectively 10mu g / mL, 10mu g / mL and 14mu g / mL, and the inhabitation ratio can be increased along with the prolonging of time and increase of concentration; and the change of cell apoptosis morphology can be observed under a light microscope.
Description
technical field [0001] The invention relates to the protein extract of Pleurotus ostreatus and its anti-tumor application. Background technique [0002] Red Pleurotus (Pleurotus djamor), also known as Red Pleurotus, Pleurotus genus, belongs to Basidiomycota, Phytomycetes, Agaricaceae, Pleurotaceae, and Pleurotus. The mycelia of Pleurotus ostreatus grows fast and has a high yield. It is a rare mushroom that is both edible and ornamental. The research on Pleurotus ostreatus is mostly found in the breeding of Pleurotus ostreatus species and the exploration of effective component extraction methods. He Xinsheng et al. used nitrosoguanidine (NTG) as a mutagen in the research on the chemical mutation breeding of Pleurotus ostreatus. A large number of new strains were found in the treatment of Pleurotus eryngii. At the same time, the effects of carbon source, nitrogen source, carbon-nitrogen ratio, temperature, pH value, etc. Development, agronomic traits, cultivation methods, cu...
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