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Flammulina velutipes protein extract and applications thereof for resisting tumor

A protein extract, Flammulina velutipes technology, applied in anti-tumor drugs, peptide/protein components, applications, etc., can solve problems such as blanks

Inactive Publication Date: 2013-09-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, it is still blank to isolate protein substances from Flammulina velutipes and study its inhibition of tumors in vitro

Method used

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  • Flammulina velutipes protein extract and applications thereof for resisting tumor
  • Flammulina velutipes protein extract and applications thereof for resisting tumor
  • Flammulina velutipes protein extract and applications thereof for resisting tumor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the preparation of Flammulina velutipes protein extract

[0033] 1. Prepare the fruiting bodies of Flammulina velutipes

[0034] Inoculate Flammulina velutipes hybrid 19CFCC89540 slant bacteria into the first-level medium for activation, culture at a constant temperature at 25°C, and inoculate it into the second-level medium after the mycelium is full of the test tube, in a constant-temperature culture room at 25°C Cultivate until the hyphae are overgrown, inoculate the secondary species into the cultivation bag with the cultivation medium for germination at 25°C, scratch the fungus after the strain is full of the cultivation bag, and move it into the greenhouse. The fruiting conditions are maintained The humidity is above 90%, and the temperature is 10-15°C. Collect the fruiting body of the first tide, and obtain the fruiting body of Flammulina velutipes hybrid 19CFCC89540.

[0035] Wherein, the culture medium used in this experiment is as follows:

[0...

Embodiment 2

[0043] Embodiment 2, Flammulina velutipes protein extract inhibits tumor cell proliferation experiment

[0044] 1.1 Tested cell lines

[0045] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).

[0046] 1.2 Experimental method

[0047] 1.2.1 Cell lines and cell culture

[0048] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%, and the volume...

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Abstract

The invention discloses a flammulina velutipes protein extract and applications thereof for resisting tumor. The flammulina velutipes protein extract is prepared by adopting the method comprising the following steps of: 1) grinding flammulina velutipes fruiting body, then leaching with water, and collecting water-soluble matters to obtain water-soluble flammulina velutipes extract; and 2) carrying out 80% saturation of ammonium sulfate precipitation to the water-soluble flammulina velutipes extract, collecting the precipitate, and dialyzing the precipitate with deionized water to obtain the flammulina velutipes protein extract. After the flammulina velutipes protein extract is used for treating human liver cancer HepG2 cell, human breast cancer MCF-7 cell and human lung adenocarcinoma cell A-549 for 72 hours, the IC50 (50% inhibiting concentration) values are respectively 8mu g / mL, 8mu g / mL and 8mu g / mL, and the inhabitation ratio can be increased along with the prolonging of time and increase of concentration; and the change of cell apoptosis morphology can be observed under a light microscope.

Description

technical field [0001] The invention relates to a protein extract of Flammulina velutipes and its anti-tumor application. Background technique [0002] Flammulina velutipes (Flammulina velutipes), also known as Pleurotus velutipes, Structural bacteria, Blue barella, Trichomonas velutipes, belongs to Basidiomycota, Phytomycetes, Agaricaceae, White mushroom family, Flammulina genus. Flammulina velutipes has a smooth and tender cap, slender and crisp stipe, beautiful shape, and delicious taste. It is a famous edible and medicinal fungus and ornamental fungus in the world. It has high nutritional value and medicinal value, and has broad development prospects. Flammulina velutipes is known as "enhancing wisdom mushroom" because it is rich in protein and various minerals and vitamins. [0003] Sun Yanyan summarized the various links of her cultivation technology (including cultivation season, variety selection, compost preparation, cultivation management, harvesting and postharve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61K36/07C07K2/00C07K1/30C07K1/36C07K1/34A61P35/00A23L1/28A23L1/305
Inventor 赵爽刘宇耿小丽许峰王守现陈杰杨娟娟王兰青尹昭坤
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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