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Pycnporus cinnabarnus protein extract and anti-tumor application thereof

A technology of protein extract and Vermilion dense porosity, applied in the direction of antineoplastic drugs, peptide/protein components, applications, etc.

Active Publication Date: 2015-05-27
北京济全生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on extracting protein substances from Pycnoporus cinnabarirus to directly kill tumor cells is still blank

Method used

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  • Pycnporus cinnabarnus protein extract and anti-tumor application thereof
  • Pycnporus cinnabarnus protein extract and anti-tumor application thereof
  • Pycnporus cinnabarnus protein extract and anti-tumor application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of Mypopora vermilion protein extract

[0032] 1. Prepare the fruiting body of Mycoporus vermilion

[0033] Inoculate Pycnoporus cinnabarirus (Pycnoporus cinnabarirus) CFCC89594 slant strains into the primary medium for activation, and cultivate at a constant temperature of 25°C. Cultivate in the culture room until the hyphae are overgrown, inoculate the secondary species into the cultivation bag with the cultivation medium at 25°C for fungal growth, and then scratch the fungus after the fungus is full of the cultivation bag and move it into the greenhouse for fruiting Conditions keep the humidity above 90% and the temperature at 24-28°C, collect the first tidal fruiting bodies to obtain the fruiting bodies of Pycnoporus cinnabarirus CFCC89594.

[0034] Wherein, the culture medium used in this experiment is as follows:

[0035] Primary seed medium: 200g potato, 20g glucose, 20g agar powder, 3g KH 2 PO 4 , 10mg Vitamin B 1 , 5g peptone,...

Embodiment 2

[0042] Example 2, Experiment of inhibiting tumor cell proliferation by the protein extract of Mypopora vermilion

[0043] 1.1 Tested cell lines

[0044] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).

[0045] 1.2 Experimental method

[0046] 1.2.1 Cell lines and cell culture

[0047] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%, and th...

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Abstract

The invention discloses a pycnporus cinnabarnus protein extract and anti-tumor application thereof. The pycnporus cinnabarnus protein extract is prepared according to the following steps of: 1) extracting by water after crushing pycnporus cinnabarnus sporocarp, and collecting water-soluble substances to obtain a pycnporus cinnabarnus water-soluble extract; 2) carrying out ammonium sulfate precipitation on the pycnporus cinnabarnus water-soluble extract of which the saturation level is 80%, collecting sediments, thereby obtaining the pycnporus cinnabarnus protein extract after dialyzing the sediment by deionized water. The IC50 values are 12 mu g / ml, 6 mu g / ml and 4 mu g / ml respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a human lung adenocarcinoma A-549 cell are processed by the pycnporus cinnabarnus protein extract; the inhibition ratio is increased along with time expansion and increase of the concentration; apoptosis morphology change of the cells can be observed under a light microscope.

Description

technical field [0001] The invention relates to a protein extract of Mypopora vermilion and its anti-tumor application. Background technique [0002] Pycnoporus cinnabarirus is also known as Rhodotraceae, Cinnabari, Cinnabar, and Red Tracy. In terms of classification, it belongs to the order of Phyllomycetes, Polyporaceae, and Trametes. Its fruiting bodies have the effects of clearing away heat, detoxification, and anti-inflammation. At present, the research on Microporia vermilion mainly focuses on the optimization of fermentation process and the research on the function of active ingredients. In order to obtain exopolysaccharides from Rhodotrametes, Cao Youqin studied the process conditions for producing exopolysaccharides in submerged culture of Rhodotramets, and optimized the fermentation medium formula. Rong Weiyan explored the two-state fermentation method and polysaccharide extraction process of Rhodotramets. The test results show that the two-state fermentation has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61K38/00A23L1/28A23L1/305A61P35/00
Inventor 赵爽王守现刘宇王兰青许峰耿小丽
Owner 北京济全生物科技有限公司
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