Pleurotus abalones protein extract and applications thereof for resisting tumor
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A technology of protein extract and abalone mushroom, applied in the direction of anti-tumor drugs, peptide/protein components, applications, etc., can solve the problem of tumor cell killing and so on
Inactive Publication Date: 2013-09-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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At present, the research on the separation and purification of protein from the fruiting body of abalone mushroom and its killing of tumor cells is still blank
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Embodiment 1
[0032] Embodiment 1, the preparation of oyster mushroom protein extract
[0033] 1. Prepare the fruiting body of abalone mushroom
[0034] Inoculate Pleurotus abalonus (Pleurotus abalonus) CFCC89572 slant bacteria into the primary medium for activation, and cultivate at a constant temperature of 25°C. Cultivate until the hyphae are overgrown, inoculate the secondary species into the cultivation bag with the cultivation medium for germination at 25°C, scratch the fungus after the strain is full of the cultivation bag, and move it into the greenhouse. The fruiting conditions are maintained The humidity is above 90%, and the temperature is 15-30°C. The first tide fruiting body is collected to obtain the fruiting body of Pleurotus abalonus CFCC89572.
[0035] Wherein, the culture medium used in this experiment is as follows:
[0043] Embodiment 2, the experiment of protein extract of oyster mushroom suppresses tumor cell proliferation
[0044] 1.1 Tested cell lines
[0045] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).
[0046] 1.2 Experimental method
[0047] 1.2.1 Cell lines and cell culture
[0048] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%, and the...
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Abstract
The invention discloses a pleurotus abalones protein extract and applications thereof for resisting tumor. The pleurotus abalones protein extract is prepared by adopting the method comprising the following steps of: 1) grinding pleurotus abalones fruiting body, then leaching with water, and collecting water-soluble matters to obtain water-soluble pleurotus abalones extract; and 2) carrying out 80% saturation of ammonium sulfate precipitation to the water-soluble pleurotus abalones extract, collecting the precipitate, and dialyzing the precipitate with deionized water to obtain the pleurotus abalones protein extract. After the pleurotus abalones protein extract is used for treating human liver cancer HepG2 cell, human breast cancer MCF-7 cell and human lung adenocarcinoma A-549 cell for 72 hours, the IC50 (50% inhibiting concentration) values are respectively 6mu g / mL, 6mu g / mL and 12mu g / mL, and the inhabitation ratio can be increased along with the prolonging of time and increase of concentration; and the change of cell apoptosis morphology can be observed under a light microscope.
Description
technical field [0001] The invention relates to abalone mushroom protein extract and its anti-tumor application. Background technique [0002] Abalone mushroom (Pleurotus abalonus), belongs to Basidiomycotina, Laminomycetes, Agaricaceae, Pleurotaceae, and Pleurotus genus. Abalone mushroom is also known as Taiwan oyster mushroom. , is a kind of edible fungus with high temperature fruiting. Because fresh mushrooms are resistant to storage and are suitable for canning, they are famous edible fungi in subtropical regions. [0003] At present, the research on abalone mushrooms is mainly focused on the optimization of the cultivation conditions and cultivation methods of abalone mushrooms. Different formulations of compost will affect the mycelium growth rate, fruiting body traits, nutritional components and yield performance of abalone mushrooms. There are few data on the research on the activity of the fruiting body extract of abalone mushroom. Wang Changrong found that the f...
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