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Pholiota adiposa protein extract and anti-tumor application thereof

A technology of protein extracts and anti-tumor products, applied in the direction of anti-tumor drugs, peptide/protein components, applications, etc.

Active Publication Date: 2015-03-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study of anti-tumor in vitro by extracting protein substances from the fruiting body of the umbrella is still blank

Method used

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  • Pholiota adiposa protein extract and anti-tumor application thereof
  • Pholiota adiposa protein extract and anti-tumor application thereof
  • Pholiota adiposa protein extract and anti-tumor application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1, umbrella protein extract

[0033] 1. Preparation of the fruiting body of the yellow umbrella

[0034] Inoculate the Pholiota adipose HS5CGMCC No.6063 slant bacteria into the primary medium for activation, and cultivate at a constant temperature of 25°C. Cultivate in a constant temperature culture room until the hyphae are overgrown, inoculate the secondary species into a cultivation bag with a cultivation medium at 25°C, and then scratch the bacteria after the cultivation bag is full and move it into the greenhouse. Mushroom conditions keep the humidity above 90% and the temperature at 18-22°C. Collect the first tide fruiting body to obtain the fruiting body of Pholiota adipose HS5 CGMCCNo.6063.

[0035] Wherein, the culture medium used in this experiment is as follows:

[0036] Primary seed medium: 200g potato, 20g glucose, 20g agar powder, 3gKH 2 PO 4 , 10mg Vitamin B 1 , 5g peptone, 1.5g MgSO 4 , 1000mL distilled water, autoclav...

Embodiment 2

[0043] Embodiment 2, the experiment that the protein extract of the umbrella chrysanthemum inhibits tumor cell proliferation

[0044] 1.1 Tested cell lines

[0045] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).

[0046] 1.2 Experimental method

[0047] 1.2.1 Cell lines and cell culture

[0048] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two...

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Abstract

The invention discloses a pholiota adiposa protein extract and anti-tumor application thereof. The pholiota adiposa protein extract is prepared according to the method comprising the following steps of 1) extracting by water after crushing pholiota adiposa sporocarp, collecting a water-soluble matter to obtain a pholiota adiposa water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the pholiota adiposa water-soluble extract, collecting sediments, and obtaining the pholiota adiposa protein extract after dialyzing the sediments by deionized water. IC50 values are 30 mug / mL, 20 mug / mL, and 20 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the pholiota adiposa protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.

Description

technical field [0001] The present invention relates to the protein extract of chrysanthemum umbrella and its anti-tumor application. Background technique [0002] Yellow umbrella, also known as Pholiota adipose, willow mushroom, yellow mushroom, yellow willow mushroom, etc., belongs to Basidiomycota, Phytomycetes, Agaricaceae, Strophariaceae, and Stropharia. The yellow umbrella is rich in nutrients and has a significant effect in improving immune function. It is currently a food and medicinal fungus with development potential. [0003] At present, the research work on the yellow umbrella mainly includes the optimization of the cultivation conditions of the yellow umbrella, the nutritional value of the yellow umbrella and so on. Ou Xiuyuan et al. used plate culture and liquid culture methods to study the effects of different carbon sources and their concentrations on the mycelia growth and exopolysaccharide production of the yellow mushroom, and determined the carbon source...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61K38/00A23L1/28A23L1/305A61P35/00
Inventor 赵爽王守现刘宇许峰耿小丽王兰青
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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