Pleurotus citrinopileatus protein extractive and application thereof in tumor resisting
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A technology of protein extract and Ulmus chrysanthemum, applied in the direction of anti-tumor drugs, peptide/protein components, applications, etc., can solve the problems of tumor suppression and immune activity research that are still blank
Active Publication Date: 2013-09-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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However, the study on the isolation and purification of protein from the fruiting body
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Embodiment 1
[0032] Embodiment 1, the preparation of the protein extract of yellow mushroom
[0033] 1. Preparation of the fruiting body of Ulmus chrysanthemum
[0034] Inoculate the slant strain of Pleurotus citrinopileatus CFCC89573 into the primary medium for activation, culture at a constant temperature of 25°C, and inoculate it into the secondary medium after the mycelium is full of the test tube, and cultivate at a constant temperature of 25°C Cultivate in the room until the hyphae are overgrown, inoculate the second-grade species into the cultivation bag with the cultivation medium for germination at 25°C, scratch the fungus after the strain is full of the cultivation bag, and move it into the greenhouse. Keep the humidity above 90% and the temperature at 16°C, collect the fruiting body of the first tide, and obtain the fruiting body of Pleurotus citrinopileatus CFCC89573.
[0035] Wherein, the culture medium used in this experiment is as follows:
[0036]Primary seed medium: 200g...
Embodiment 2
[0043] Embodiment 2, the experiment that the protein extract of Ulmus chrysanthemum inhibits tumor cell proliferation
[0044] 1.1 Tested cell lines
[0045] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).
[0046] 1.2 Experimental method
[0047] 1.2.1 Cell lines and cell culture
[0048] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1%,...
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Abstract
The invention discloses a Pleurotus citrinopileatus protein extractive and an application thereof in tumor resisting and particularly relates to the application of the Pleurotus citrinopileatus protein extractive in preparation of anti-tumor products or anti-tumor cell products. The Pleurotus citrinopileatus protein extractive is prepared by a method including the following steps of: (1) carrying out lixiviating by utilizing water after crushing Pleurotus citrinopileatus sporocarp, collecting water-soluble matters, so as to obtain Pleurotus citrinopileatus water-soluble extractives; and (2) carrying out ammonium sulfate precipitation with the saturability of 80% on the Pleurotus citrinopileatus water-soluble extractives, collecting precipitates, and obtaining the Pleurotus citrinopileatus protein extractive after carrying out deionized water dialysis on the precipitates. After the Pleurotus citrinopileatus protein extractive is used for processing human liver cancer HepG cells, human breast cancer MCF-7 cells and human lung cancer A-549 cells for 72 hours, IC50 (half maximal inhibitory concentration) values respectively are 1.0 microgrammes per milliliter, 0.45 microgrammes per milliliter and 1.15 microgrammes per milliliter, and inhibition ratios are increased along the prolonging of the time and the increasing of the concentrations.
Description
technical field [0001] The present invention relates to the protein extract of elm yellow mushroom and its anti-tumor application. Background technique [0002] At present, according to the statistics of the World Health Organization (WHO), 10 million people in the world suffer from cancer every year, and about 60,000 people die from cancer, accounting for 12% of the global death toll. There are 1.8 million new cancer patients and 1.4 million deaths every year in my country. On average, 1.3 people die of cancer every 3 minutes, and the incidence of cancer is on the rise. Last year, the number of new cancer cases was about 1.39 million, accounting for the world's cancer 17% of the total, basically close to 1 / 5. Among them, lung cancer is one of the most common and deadly malignant tumors in the world, and its incidence rate growth rate ranks first among all malignant tumors. Liver cancer has a relatively high incidence rate in the adult population in my country, and is a comm...
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