8 results about "LENTINULA EDODES MYCELIUM" patented technology

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The invention discloses a lentinus edodes MAPK family gene Le-10004182, and the sequence of the lentinus edodes MAPK family gene Le-10004182 is shown as SEQ ID NO. 1. An overexpression vector or an interference vector of the gene Le-10004182 is constructed through a cDNA (complementary deoxyribonucleic acid) sequence of the gene Le-10004182, and the overexpression vector or the interference vector is transferred into mushroom mycelia through an agrobacterium tumefaciens-mediated genetic transformation method, so that the biomass of the mushroom mycelia is regulated and controlled. The lentinus edodes MAPK family gene Le-10004182 and the regulation and control of the lentinus edodes MAPK family gene Le-10004182 on the growth of lentinus edodes mycelia provide a new solution thought for further analysis of the growth and development of the lentinus edodes mycelia and variety improvement.

The invention discloses bionic shark fin flavor essence, a probiotic microcapsule, a preparation process of the bionic shark fin flavor essence and the probiotic microcapsule and an instant seasoning. The bionic shark fin flavor essence is prepared from the following components in percentage by weight: 0.5%-1.5% of L-alanine, 0.3%-1.0% of glycine, 0.05%-0.4% of disodium 5 '-inosinate, 0.05%-0.4% of disodium 5'-guanylate and 0.1%-0.8% of sodium succinate. 1%-5% of yeast extract, 2%-8% of mushroom mycelium fermentation liquor, 0.05%-0.3% of ginger oleoresin and 0.03%-0.25% of garlic oleoresin; 20%-55% of propylene glycol and 5%-15% of sorbitol; 0.2%-3% of probiotic microcapsules subjected to embedding treatment; and the balance of deionized water. According to the bionic shark fin flavor essence provided by the invention, through an elaborately designed plant base and fermentation source formula, the dependence of the traditional shark fin flavor on seafood extracts is completely avoided, the seafood allergen risk is completely eradicated, and the consumption requirements of special crowds are met.

The present invention relates to a composition with good permeability and whitening effect and a preparation method thereof, belonging to the technical field of skin care products. The composition of the present invention comprises the following components: by weight percentage, 0.5-3% niacinamide, 0.1-0.5% ascorbyl glucoside, 0.1-1% tetrahydromethylpyrimidine carboxylic acid, 0.1-10% shiitake mushroom mycelium extract solution, 0.05-5% raspberry extract solution, 0.05-5% wheat germ extract solution, 0.01-0.1% decapeptide-4 solution, 0.001-1% borneol solution, 0.05-1.2% preservative, 0.05-2% thickener and the balance deionized water. The active ingredients of the composition prepared by the present invention have good permeability, excellent whitening effect, and have very good market application prospects.

The invention relates to the technical field of cosmetic raw material preparation, in particular to a preparation method of mushroom mycelium fermentation liquor with a skin barrier repairing function, and solves the problems of unstable metabolites and poor batch repeatability caused by unknown mushroom strain sources and random culture parameters in the prior art. The preparation method of the shiitake mushroom mycelium fermentation liquor with the skin barrier repairing function comprises the steps that shiitake mushroom strains are inoculated into a slant culture medium and cultured for 7-10 days at the temperature of 20-25 DEG C, the lawn is obtained, and the slant culture medium is prepared from 18-24 g / L of potato glucose water, 15-20 g / L of agar powder and the balance water. By optimizing a culture medium formula and key process parameters, stable components, high activity and good batch consistency of the fermentation liquor are realized, and the problem of quality fluctuation caused by strain disorder and extensive process is effectively solved.

This invention belongs to the field of health food and discloses a preparation process and application of a compound peptide that enhances immunity. The peptide is composed of the following components: 5-15 parts yak bone peptide, 3-10 parts marine collagen oligopeptides, 2-8 parts soybean protein peptide, 1-5 parts cordyceps peptide, 1-5 parts wolfberry peptide, 0.5-3 parts sea cucumber intestinal ovum peptide, 0.3-2 parts yeast β-glucan, 0.01-0.1 parts selenomethionine, 0.2-1.5 parts shiitake mycelium polysaccharide, 0.1-1 parts elderberry extract, 0.1-1 parts echinacea extract, 0.05-0.5 parts zinc-lactalbumin complex, and 0.03-0.3 parts ferrous lactate. This invention proposes a preparation process and application of a compound peptide that enhances immunity. By using specific component ratios and manufacturing processes, it not only has excellent immune activation capabilities but also comprehensively regulates the body's immune function. It can be used to prepare foods or health products that enhance immunity.

Provided is a hypha composite material including: a fiber oriented in a first direction; and a mycelium, in which the first direction is a longitudinal direction of the fiber, and the fiber and the mycelium are integrated with cultured and grown hyphae. An aspect of the hypha composite material is a laminate in which hypha composite sheets are laminated. It is preferable that the mycelium is a mycelium of Lentinula edodes. In the laminate, at least a first hypha composite sheet and a second hypha composite sheet are laminated, and an orientation direction of a fiber of the first hypha composite sheet intersects an orientation direction of a fiber of the second hypha composite sheet.

The present application relates to a kind of recombinant expression vectors and the method for improving lentinula edodes heat resistance and trichoderma resistance, the recombinant expression vector carries the gene of purpose as shown in SEQ ID NO.2, the method is into the lentinula edodes strain carrying LeV-HKB virus of the recombinant expression vector, the recombinant expression vector carrying the gene of purpose as shown in SEQ ID NO.2 can promote the growth of lentinula edodes mycelium and improve the heat resistance and trichoderma resistance of strain after thermal stress, using the method can simultaneously improve the heat resistance of lentinula edodes and the resistance to trichoderma, it is beneficial to reduce the economic loss caused by high temperature thermal stress or deep green trichoderma infection in lentinula edodes planting.