60 results about "LENTINULA EDODES MYCELIUM" patented technology

A method for preparing a feed by taking edible fungi mycelia as raw materials comprises the following specific steps: culturing ganoderma mycelium, pleurotus ostreatus mycelium and champignon mycelium according to a method for preparing cultivated species, taking the three mycelia as main raw materials for preparing a complete feed, and combining the main raw materials with maize meal, pulse flour and fish meal to form the complete feed. Though the implementation of the method, all mycelia are mainly produced from crop straws and various leftovers, so that the production cost of the mycelia are extremely low. The ganoderma mycelium, pleurotus ostreatus mycelium and champignon mycelium which contain abounding proteins and various amino acids are taken as the raw materials, and can be prepared into the low-cost feed which can be directly absorbed by animals, and is comprehensive in nutrition and high in palatability through improvement and conversion, Meanwhile, the feed contains various effective components of ganoderma, pleurotus ostreatus and champignon and thus can enhance animal immunity when fed to animals. Moreover, meat products of the animals not only have functions of medicated food but also can serve as a novel health-care food to maintain human health.

The invention relates to a composition containing mushroom mycelium polysaccharides and applications thereof. The composition comprises following components in percentage by weight: 60% to 80% of mushroom mycelium polysaccharides, 10% to 20% of black rice polypeptide, and 10% to 20% of bird cherry anthocyanin. The composition containing mushroom mycelium polysaccharides can be used to produce anti-fatigue drugs, anti-fatigue health food and feed.

The invention discloses a making method of a mushroom health milk beverage with a function of replenishing calcium, relating to a making method of beverages, and particularly relating to a making method of a mushroom health milk beverage with a function of replenishing calcium. The making method comprises the following steps: firstly, preparing a mushroom mycelium culture solution, then filtering to obtain a wet mushroom mycelium, airing the wet mushroom mycelium in the sun, adding the aired mushroom mycelium in milk, uniformly mixing, enabling the mixed solution to pass a colloid mill, then homogenizing to obtain a mushroom mycelium culture solution, weighing a certain amount of mushroom mycelium culture solution, adding olive oil dissolved with monoglyceride in the mushroom mycelium culture solution, blending, homogenizing, filling, sealing, and pasteurizing to obtain the mushroom health milk beverage. The health beverage disclosed by the invention contains a large quantity of vitamins D, is capable of promoting the absorption of calcium, and can be used for effectively preventing and treating rickets of children and osteoporosis of the adults.

The invention provides a mushroom mycelium solid medium and a method for culturing mushroom mycelium by using the same. The mushroom mycelium solid medium is prepared from the following raw materials proportionally: 35-42% of bagasse, 3.5-7% of rice bran, 3.5-7% of corn starch, 0.4-0.6% of gypsum, 0.4-0.6% of sugar, 0.4-0.6% of salt and 45-55% of water. The method for culturing the mushroom mycelium by using the mushroom mycelium solid medium comprises the following steps of: weighing the raw materials in proportion, and mixing the raw materials to prepare into the mushroom mycelium solid medium; bagging and sterilizing the prepared mushroom mycelium solid medium; inoculating the mushroom mycelium on the solid medium; and placing the solid medium in a spawn-running room for carrying out spawn running. The solid medium provided by the invention is used for culturing the mushroom mycelium, so that the production technology is simple and the production cost is low; and the contents of lentinan and various types of active substances in the cultured mushroom mycelium are high, so that the mushroom mycelium solid medium and the method are especially suitable for industrialized production and application.

The invention discloses a radial flow chromatography separating method of mushroom mycelia polysaccharides, comprising the following steps of: crushing mushroom mycelia; degreasing and extracting biological active polysaccharide with water at room temperature; removing protein; dialyzing to remove salt; purifying the radial flow chromatography; and freeze drying, wherein eluent used in the purification of the radial flow chromatography is one of a Tris-hydrochloric acid buffer solution, a phosphate buffer solution and a barbital-hydrochloric acid buffer solution; and anion exchange filler is agarose gel with an aglycone group of diethyl aminoethyl. The separating method does not influence a natural structure and the separating effect of the mushroom mycelia polysaccharides; the obtained mushroom mycelia polysaccharides has high content, retains the bioactivity and can be used as a pharmaceutical raw material (such as injection and the like); and the method has short process, low energy consumption and low cost, thereby ensuring the stable and quick separation of polysaccharides and being convenient for indusial production.

The invention discloses a composition for treating diabetes and having the function of lowering blood sugar, and relates to a preparation method for the composition. The composition is prepared from the following components in percentage by weight: 25-40% of coprinus comatus mycelium, 8-15% of pleurotus citrinopileatus mycelium, 8-15% of grifola frondosa mycelium, 4-10% of Chinese caterpillar fungus mycelium, 3-10% of tricholoma matsutake mycelium, 3-10% of cypress mycelium, 3-10% of glossy ganoderma mycelium, 1-5% of fragrant mushroom mycelium, 1-5% of black fungus mycelium, 1-5% of needle mushroom mycelium, 3-10% of pholiota nameko mycelium, 1-5% of versicolor mushroom mycelium, and 1-4% of gastrodia tubers. The product disclosed by the invention has the effects of improving the immunity of human bodies, safety, health and lowering the blood sugar, and can be used for treating the diabetes.

The inhibitor of Hepatitis B virus and HIV activity according to the present invention comprises a Lentinus edodes mycelium extract obtained by the steps of inoculating Lentinus edodes fungus in a solid culture medium comprising bagasse, then disentangling the solid culture medium containing the proliferated mycelium, adding water and at least one enzyme selected from a group consisting of cellulase, protease and glucosidase to the disentangled solid culture medium with keeping the solid culture medium at 30 to 50° C., grinding and milling the solid culture medium in the presence of the enzyme to obtain a Lentinus edodes mycelium extract, and then heating the extract to a temperature of not higher than 95° C. to inactivate the enzyme and sterilize the extract.

The invention discloses a production method for extracting mushroom mycelium of lentinan. The mushroom mycelium is obtained by the following seven steps: weighing materials; pretreating base materials; mixing; bagging and sterilizing; inoculating and cultivating; carrying out spawn running management; removing a bag and harvesting. Compared with the prior art, the production method disclosed by the invention has the advantages that through fermentation pretreatment on the base materials, coarse lignocellulose with a macromolecular structure is transformed into fine lignocellulose with a micromolecular structure; effective utilization of hyphae during cultivation of the mycelium is facilitated; the biotransformation utilization rate of the mycelium at the cultivation stage is increased, and reaches about 70%; the cultivation time of the mycelium is shortened; the production efficiency is improved; the production cost is reduced; comprehensive utilization of waste in industrial and agricultural production is achieved; the environment is protected.

The invention publishes a producing method of a kind of filar nutrient wine of mushroom. The process is; (1) use dry sweet potato corn powdr as material; (2) wash the material, then peel it off, cut it into block and insolate it; (3) marinate treated material with water and then insert corn powder and mix round then evenly; (4) load the material in the bag; (5) sterilize in high-pressure sterilizing boiler; (6) transplant the mushroom; (7) transfer the material after having been transplanted to cultivate room without light; (8) insert the foregoing production and fragrant instilled wine in hermetic container; (9) filtrate by using gauze; (10) examine the quality and pack. In short, it has a host of advantages such as siple producing method, short producing time, small investment and low cost. Moreover, it provides an effective way to produce crude farm produce.

The invention discloses a method for producing shiitake mushroom mycelia by using ginkgo leaves. The shiitake mushroom mycelia are obtained through the seven steps of cleaning, material weighing, pretreatment of base materials, mixing, bagging sterilization, inoculated culture, spawn running management and bagging removal harvest. Compared with the prior art, the method for producing the shiitake mushroom mycelia by using the ginkgo leaves has the advantages that the ginkgo leaves are used as the main base materials, the cultured shiitake mushroom mycelia have a medical effect while the fallen leaves are treated, through fermentation pretreatment of the base materials, the culture time of the mycelia is shortened, production efficiency is improved, production cost is reduced, waste of industrial and agricultural production is comprehensively utilized, and the environment is protected.

The invention relates to a preparation method and application of a lentinus edodes mycelium bio-based composite material, and belongs to the technical field of bio-based materials. The preparation method comprises the following steps: preparing a biological culture medium; inoculating lentinus edodes strains into a biological culture medium, and carrying out primary mycelium culture; crushing a product obtained by the primary mycelium culture until no white mycelium appears, adding water and wheat flour, uniformly mixing, putting the mixture into a mold, and carrying out secondary mycelium culture to obtain a semi-finished product; baking, shaping and sterilizing the semi-finished product, and then taking out and airing the semi-finished product to obtain the lentinus edodes mycelium bio-based composite material. The preparation method of the lentinus edodes mycelium bio-based composite material is good in forming effect and excellent in mechanical property.

The invention relates to microorganism fermentation, in particular to a shiitake culture and a method for simply preparing a shiitake mycelium with same. The method comprises the following process steps: inoculating a shiitake culture Lentinus edodes CGMCC NO. 16991 with a sterilized fermentation culture medium, and carrying out deep gas introduction fermentation so as to prepare a deep culture fermentation liquor; inoculating the deep culture fermentation liquor with a mycelium generation culture liquid, and carrying out tissue culture and bottle culture, so as to obtain the shiitake mycelium. The method is capable of solving problems that in the prior art a fermentation method is high in shiitake mycelium production investment, low in yield, high in technical difficulty, high in equipment investment, and the like, and has the advantages of being stable in product quality and process, low in production cost, and the like.

Disclosed are compositions and methods for their use that include a combination of Leontopodium alpinum extract, Halidrys siliquosa extract, vegetable amino acids, niacinamide, hexylresorcinol, Pinus pinaster extract, Betula alba extract, Albizia julibrissin bark extract, hydrolyzed Candida saitoana extract, Lentinus edodes mycelium extract, and / or Opuntia tuna fruit extract.

The invention relates to hericium mycelium biscuits and a making method thereof. The hericium mycelium biscuits are made from hericium mycelium blocks, matsutake fermentation liquid, black fungus fermentation liquid, morchella fermentation liquid, lentinula edodes fermentation liquid, fructooligosaccharide, dietary fiber, vegetable oil, soybean powder and sodium bicarbonate. The making method comprises the steps of powder mixing, stamping molding through models, baking and matsutake mycelium spraying. The biscuits contain hericium mycelia, matsutake mycelia, black fungus mycelia, morchella mycelia and lentinula edodes mycelia and have the effects of strengthening the spleen and the stomach, tonifying qi and blood, promoting intelligence and soothing the nerves, maintaining beauty and keeping young, tonifying the kidney, strengthening yang, reinforcing the brain, refreshing the spirit, resisting cancers, enhancing the immunity of the organism and the like.

The invention belongs to the technical field of biological culture and particularly relates to method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture. The method comprises the steps of taking lignin as an exogenous additive, and preparing an improved potato dextrose broth medium containing a certain concentration of lignin; inoculating a lentinus edodes mycelium, culturing the lentinus edodes mycelium for a period of time and then collecting the lentinus edodes mycelium; and extracting and obtaining phenolic acid substances from the collected lentinus edodes mycelium. According to the method provided by the invention, growth of the lentinus edodes mycelium and biotransformation of phenolic compounds can be promoted through adding an appropriate concentration of lignin.

The invention discloses a cultivation method capable of improving the content of polysaccharides in shiitake mushroom hyphae. The cultivation method comprises the following steps: treating a base material, preparing a matrix material, bagging, sterilizing at high temperature, inoculating and culturing the hyphae. A biological growth regulator is added in any one or any two of steps of preparing the matrix material, inoculating and culturing the hyphae; the biological growth regulator is formed by combining any one or two of oleic acid, alpha-naphthylacetic acid and 3-indolebutyric acid. By adopting the the cultivation method capable of improving the content of the polysaccharides in the shiitake mushroom hyphae, disclosed by the invention, the value of the shiitake mushroom hyphae can be improved; meanwhile, waste ginkgo leaf residues obtained by manufacturing enterprises of ginkgo leaf extract are sufficiently utilized, so that the production cost of mushroom growers is reduced and the income is improved; the cultivation method further has the characteristics of simplicity in operation technology, convenience for operation and capability of sufficiently utilizing resources.

The invention relates to a preparation method of a feed additive and especially relates to a preparation method of a feed additive for preventing and treating a soft-shelled turtle soft-shell disease. The preparation method comprises the following steps of preparing a mushroom mycelium culture solution, carrying out centrifugation to obtain mushroom mycelia, spreading the mushroom mycelia on a shallow tray, drying them in the sun, uniformly mixing the dried mushroom mycelia, crab shell powder, eggshell powder and shell powder according to a ratio of 1: 3: 3: 3, and carrying out crushing, granulation and packaging. The feed additive for preventing and treating the soft-shelled turtle soft-shell disease contains a large amount of calcium and vitamin D. Through use of the feed additive, calcium supplement is realized and the soft-shell disease can be effectively prevented and treated. In feeding, soft-shelled turtle complete feed is added with 2% of the feed additive.

The invention provides a production technology of an oral health lentinan liquid. The technology is characterized in that the technology comprises the following steps: 1, carrying out rotary oscillation culturing of shiitake liquid strains in a culturing medium by using a triangular flask in a fermentation tank, cooling, disinfecting at 121DEG C for 80min, cooling, inoculating 3% of the shiitake liquid strains, and carrying out ventilated culturing at 25DEG C for 6d to obtain a shiitake mycelium stock solution, wherein the formula of the culturing medium comprises 3% of sucrose, 2% of corn flour, 0.3% of ammonium hydrogen carbonate, 0.1% of magnesium sulfate, 5% of a potato juice, and the balance purified water; 2, heating the shiitake mycelium stock solution to 75DEG C, maintaining for 35min to inactivate enzymes in the stock solution, cooling, adding zinc acetate, stirring, adjusting the pH value to 6.3 by a sodium hydroxide solution, cooling for 12h, and filtering to obtain a lentinan stock solution; and 3, adding a proper amount of honey and white sugar into the lentinan stock solution, carrying out heating filtration, carrying out fine filtration, canning, and disinfecting to obtain the oral liquid; and the technology successfully developed by a designer can be widely applied to production technologies of oral health lentinan liquids to generate significant economic and social benefits.

The invention discloses a moisturizing, tightening and repairing composition, a cosmetic and a preparation method, and relates to the field of cosmetics. The composition is prepared from plant extracts such as a CICHORIUM INTYBUS root extract, caesalpinia spinosa (CAESALPINIASPINOSA) gum, a Lentinus edodes (LENTINUS EDODES) mycelium extract, a Spanish sage herb (SAVIAHISANICA) seed extract, a lactobacillus fermentation product, a chlorella vulgaris VULGARIS extract and the like, and is prepared from the plant extracts such as the CICHORIUM INTYBUS root extract, the caesalpinia spinosa gum, the Lentinus edodes EDODES mycelium extract, the Spanish sage herb seed extract, the lactobacillus fermentation product, the chlorella vulgaris VULGARIS extract and the like. The refreshing oil ester is matched with the vegetable oil ester to effectively moisten the skin and degrade the stickiness of the formula, a refreshing and non-greasy system is achieved, multiple plant extracts are added, the dry skin is repaired, the problem of dry and itching skin in winter is solved, the skin elasticity is improved, meanwhile, the skin is astringed and relaxed, wrinkles are reduced, and diversified effects are achieved.

The invention provides a composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof. The composition comprises extractives of the following six cereals: corn, barley, Job's tears, millet, buckwheat and wheat, comprises extractives of the following 25 Chinese herbal medicines: medicated leaven, fructus hordei germinatus, radix et rhizoma rhei, cablin potchouli herb, radix glycyrrhizae, folium perillae, rhizoma atractylodis macrocephalae, rhizoma chuanxiong, radix scutellariae, chinese lizardtail rhiaomeor herb, fructus crataegi, radix angelicae sinensis, radix ginseng rubra, fructus lycii, rhizoma coptidis, radix astragali, fructus schisandrae Chinensis, concha haliotidis, herba artemisiae annuae, poria, cortex magnoliae officinalis, fructus corni, radix aucklandiae, akebia stem and fructus forsythia, comprises the following four algae extractives: laminaria japonica, scagassum, codium fragile and seaweed, and comprises the following three mushroom mycelia: tricholoma gambosum mycelium, lentinus edodes mycelium and cordyceps sinensis mycelium. The composition is applicable as a forage additive. The composition has the advantages of promoting growth of mammalia, poultry, fish, arthropods and most of animals, enhancing resistance and the like, and especially has special efficacy for preventing white spot syndrome of crustacea.

The invention discloses lentinus edodes mycelium fermented tea. According to the lentinus edodes mycelium fermented tea, tea leaves are used as raw materials, and the lentinus edodes mycelium fermented tea is prepared through the following steps of inoculating original species or culturing species of lentinus edodes to the tea leaves, performing sterilization, performing culturing, performing inactivating and performing drying. The invention further discloses a processing method of the lentinus edodes mycelium fermented tea. Under the premise that no culturing auxiliary materials are added, the tea leaves are used as culture mediums for growth of lentinus edodes mycelium. In the culturing process of the lentinus edodes mycelium, a large quantity of amino acid substances and polysaccharide substances can be synthesized, so that the fresh and brisk degree of the taste of products is increased, and the abundance of inclusions is improved. Tea polyphenol substances are naturally oxidized, so that the bitterness is reduced, and the quality of the tea leaves as the culture mediums is improved. The lentinus edodes species are medical and edible dual-purpose mushrooms, so that the safety is high. Compared with current naturally-inoculated tea leaves, the produced tea leaves have the advantages that the mushroom species of the produced tea leaves are stable and safe in source, and the product consistency of the mycelium fermented tea products is good. A large amount of the lentinus edodes mycelium exists in the tea leaves in the fermentation process, so that the lentinus edodes mycelium fermented tea also has the efficacies of regulating the function of the stomach, invigorating the spleen, supplementing qi, and tonifying the kidney.

The invention discloses a laccase preparation method, which comprises the steps of: (1) inoculating a solid spawn of an inclined medium inoculated with Lentinus tigrinus with a preservation number of CGMCC No.8301 into a liquid seed medium to prepare a seed liquid; (2) inoculating the seed liquid into a fermentation medium, which is in a 500mL triangular flask with a liquid loading volume of 100-250mL, and conducting culture at 25-35DEG C in a shaking table of 150-350rpm for 7-20 days so as to obtain Lentinus tigrinus mycelium fermentation broth; and (3) centrifuging the mycelium fermentation broth at 10000*g for 10min, and taking the supernatant i.e. a laccase crude enzyme fluid. The method provided by the invention utilizes the edible fungus Lentinus tigrinus to conduct liquid submerged fermentation for high yielding of food grade laccase. According to the invention, the laccase in the Lentinus tigrinus mycelium fermentation broth has high enzyme activity, and the enzyme activity of laccase in the fermentation broth is up to 462.59U / mL.

The invention discloses nutritional healthcare shiitake unpolished rice which is prepared from, by weight, husked unpolished rice, micrometer granular green bean powder, micrometer granular red bean powder, micrometer granular black bean powder, wheat gluten, fish skin collagen, modified dietary fiber, egg white powder, shiitake mycelium culture liquid, tremella polysaccharide, malic acid, olive oil, isomalto-oligosaccharide, edible alkali, gelatin, konjac glucomannan, cellulose, pectinase, fungus amylase, glucoamylase and a proper amount of water. Main materials like the husked unpolished rice and micrometer granular bean plant powder and nutritional substances like shiitake mycelium culture liquid and tremella polysaccharide are adopted to jointly prepare the shiitake unpolished rice, so that the shiitake unpolished rice is rich in nutrition, high in stability, good in flavor and rich in nutritional and effective ingredients like various amino acids, shiitake polysaccharide, tremella polysaccharide and gamma aminobutyric acid and has healthcare efficacy.

The invention relates to a biological composition capable of reducing hypertension, hyperlipidemia and hyperglycemia, and also relates to a preparation method of the composition. The biological composition is composed of the components by the weight percentage: 10-14% of shiitake mushroom mycelia, 10-14% of black fungus mycelia, 10-14% of coprinus comatus mycelia, 8-11% of ganoderma lucidum mycelia, 8-11% of tricholoma matsutake mycelia, 8-11% of grifola frondosa mycelia, 2-5% of cypress mycelia, 2-5% of pleurotus eryngii mycelia, 2-5% of flammulina velutipes mycelia, 1-4% of volvaria volvacea mycelia, 1-4% of hericium erinaceus mycelia, and 2% of gastrodia elata. The product has the function of reducing hypertension, hyperlipidemia and hyperglycemia, and can be used in patients with hypertension, hyperlipidemia and hyperglycemia.

The invention discloses a rana chensinensis food for tonifying kidney, reinforcing premodial qi, resisting cancer and beautifying faces and a preparation method thereof. The rana chensinensis food is prepared from rana chensinensis, glossy ganoderma, ginseng, hairy antler, prepared rehmannia root, Chinese wolfberry, common macrocarpium fruit, cistanche, cinnamomvine, semen sesami nigrum and mushroom mycelia. The preparation method comprises the following steps of: drying the rana chensinensis in a weight ratio, and grinding into rana chensinensis powder for later use; separating the glossy ganoderma from water, steaming over slow fire for 40 minutes, taking the steamed glossy ganoderma out, airing or drying, and grinding into powder for later use; putting the dried semen sesami nigrum and the Chinese wolfberry into the mixture of the hairy antler, the ginseng, the mushroom mycelia, the cinnamomvine, the common macrocarpium fruit, the prepared rehmannia root and the cistanche, and grinding into powder; and blending the three groups of powder, and stirring uniformly. The rana chensinensis food has the effects of tonifying kidney, reinforcing premodial qi, resisting cancer and beautifying faces, and is comprehensive in nutrition, easy to absorb and obvious in effect; and compared with the conventional single rana chensinensis which is eaten directly, the rana chensinensis food has the advantages that nutritional ingredients are comprehensive and easy to absorb, and a health-care effect is obvious.

The invention relates to a lentinus edodes cabbage cultivation method. Peat, coco coir, straws, ceramsite and rice hulls form a primary substrate according to the ratio 10:2:2:1:1; lentinus edodes strains are introduced into the primary substrate, so that the lentinus edodes strains ferment and grow in the primary substrate; therefore, a fermented substrate enriched in lentinus edodes mycelium is obtained; the fermented substrate and perlite form a cultivation substrate according to the ratio 4:1; organic soilless culture of cabbages is carried out by utilization of the cultivation substrate; and the growing cabbages are lentinus edodes cabbages. By means of the lentinus edodes cabbage cultivation method disclosed by the invention, soilless culture of the cabbages is carried out by utilization of the fermented substrate enriched in lentinus edodes mycelium; therefore, the cabbages can sufficiently absorb and enrich the component of lentinus edodes in the substrate in the growth process; the growing cabbages contain multiple nutritional ingredients, particularly ergosterol and lentinan; and thus, the cabbages are good and no harm to human health.

The invention relates to a mushroom planting method, in particular to a soilless cultivation method for lentinula edodes. The method includes the steps that a cultivation hole is dug in sponge, by weight, 500-600 parts of water, 1-1.5 parts of mannan, 1-1.5 parts of chitosan oligosaccharide, 0.02-0.03 part of glycine, 1-2 parts of vitamin B1, 0.05-0.06 part of sodium glutamate, 0.2-0.23 part of monopotassium phosphate and 0.1-0.15 part of magnesium sulfate heptahydrate are weighed, mixed and blended, organic liquid fertilizer is obtained, the organic liquid fertilizer is sprayed on the sponge, lentinula edodes mycelium is planted in the cultivation hole in an inoculation mode, after inoculation is conducted for 15-20 days, lentinula edodes gets into fungus barrel color conversion and physiological ripe stages, in this period, the sponge is transferred under a yellow fluorescent lamp, the light intensity is 30-35 Lx, 2-3 days later, the light intensity of the yellow fluorescent lamp is adjusted to 50-60 Lx, and gauze on the cultivation hole is removed. The sponge and the organic liquid fertilizer are combined to cultivate the lentinula edodes, the cultivation method is simple and easy to implement, the cultivation cost is low, infectious microbes and pests cannot be generated easily in the cultivation period, other fertilizer and chemical deinsectization antibacterial agents do not need to be applied in the lentinula edodes cultivation period, and the obtained lentinula edodes is green, organic and free of pollution.

The invention discloses a culture medium for continuously preparing lentinus edodes fermentation liquid seeds and a preparation method. The culture medium contains arginine salt or arginine and soybean lecithin. The culture medium comprises the following components including, by weight (W / W), 20 to 30 percent of potatoes, 1.5 to 2.5 percent of glucose, 0.2 to 1.5 percent of the arginine salt or arginine, 0.05 to 0.35 percent of monopotassium phosphate, 0.05 to 0.20 percent of magnesium sulfate, 0.3 to 1.0 percent of the soybean lecithin, 0.0001 to 0.0005 percent of biotin, 0.005 to 0.02 percent of vitamin B1 and the balance of water, wherein the sum of the percentages is 100%. According to the culture medium, the arginine salt or arginine is added into the formula to promote growth of lentinus edodes mycelia, and the soybean lecithin is selectively added into the formula to increase oxygen supply to immobilized mycelia. Meanwhile, the fermentation culture time is shortened, the seed production period is shortened, the inoculation pollution risk is reduced, and the operation is simple and convenient.