Method for cultivating pleurotus citrinopileatus by using persimmon sawdust
A technology of pleurotus chinensis and sawdust, which is applied in the field of utilization of agricultural and forestry residues and the cultivation of edible and medicinal fungi, can solve the problems of waste of resources, environment, pollution, waste of resources, etc., to increase the speed of germination, improve the efficiency of breeding, and promote The effect of growth
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[0033] The Pleurotus aureus species used in the technical solution of the present invention is quoted from the Institute of Edible Fungi of the Shanghai Academy of Agricultural Sciences.
[0034] Pleurotus citrinopileatus Sing. belongs to Basidiomycota, Agaricomycetes, Agaricales, Trichiolomataceae, and Pleurotus. bacteria.
[0035] The technical scheme content and application effect of the present invention are described in detail below in conjunction with embodiment:
[0036] A. Screening Pleurotus aureus plants with the advantage of decomposing persimmon sawdust:
[0037]A-1. Ultraviolet mutagenesis of Pleurotus aureus species:
[0038] 1) Mycelium preparation: The medium formula is 20% peeled potato, 2% bran, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O1g / L, pH 6.0, agar 0.3%. The culture condition was 160r / min, and the temperature was 25°C in a constant temperature shaker for 7 days.
[0039] 2) Protoplast preparation:
[0040] 2-1) Place the cultured mycelium in a centrifuge ...
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