Method for preparing pleurotus citrinopileatus liquid strain through pleurotus citrinopileatus culture medium based on eupatorium adenophorum

Abstract

The invention discloses a pleurotus citrinopileatus culture medium based on eupatorium adenophorum. The pleurotus citrinopileatus culture medium is prepared from components in parts by weight as follows: ageratina adenophora, wood vinegar, seaweed polysaccharides, soluble swelling starch, peptone, nanoscale tourmaline powder, bacterial nanocellulose and the like. A pleurotus citrinopileatus liquid strain is prepared for the culture medium in combination with the ageratina adenophora, the soluble swelling starch and the like; the sources of materials are wide, and the price cost is low; besides, the ageratina adenophora is subjected to super-fine grinding and then mixed with the seaweed polysaccharides and the wood vinegar, the mixture is boiled and extracted with soft fire, and the ingredients of the culture medium are enriched; meanwhile, the stability of the liquid culture medium is promoted by means of the property of the seaweed polysaccharides, and rich nutrition and good space are provided for stock culture and expanding culture of pleurotus citrinopileatus; good nutrients and living environments are provided for mycelium pellets of the pleurotus citrinopileatus liquid strain due to addition of the nanocellulose, the distribution stability of mycelium pellets can be improved, and loss caused by mechanical pulverization of the mycelium pellets before the liquid strain is used is avoided.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a culture medium for ulm yellow mushrooms based on Eupatorium bauhinia and a preparation method for liquid strains of ulm yellow mushrooms. Background technique [0002] In the production process of yellow elm mushrooms, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and quick inoculation, and easy implementation of industrialization and large-scale production. The advantages of modernization and standardized production. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains...

AI Technical Summary

Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a serious impact on the overall quality of the strains.

Publication No. CN105309194A "New Technology for the Production of Edible Mushroom Microparticle Liquid Strain" provides a kind of edible fungus microparticle liquid strain with a mycelium ball particle diameter of 0.5mm, which increases the ventilation rate of the edible fungus liquid strain culture solution, so that the ventilation rate It is suitable at 1:0.6—0.9V / V·min. At the same time, a stirrer is installed in the aerated fermenter to make the eddy current of the culture liquid move and prolong the technical operation method of the air bubble. The method for producing the microparticle liquid strain is from the production process There is no improvement in the aspect of liquid culture medium, and the implementation is technically difficult and demanding; Publication No. CN105130516A "Method for Making Molecular-level Biological Medium" mentions that the nutrients of molecular-level biological medium can be absorbed quickly and comprehensively. Promotes the growth of liquid strains, but does not mention the effect of molecular-grade biological media on the diameter of liquid strain mycelium balls