Chinese ephedra medicinal material, and content determination method of three alkaloids in preparation thereof

Abstract

The invention relates to a Chinese ephedra medicinal material, and a content determination method of three alkaloids in a preparation thereof. The method has the characteristics that: a common reversed-phase column and a non-buffer salt which is a mobile phase of acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 1-1.5:4-4.2:94.5-95.0 are adopted; at 207+ / -2nm, contents of ephedrine hydrochloride, pseudoephedrine hydrochloride, and ephedrine methyl hydrochloride in the Chinese ephedra medicinal material and the preparation thereof are simultaneously determined. A sample is only processed through water-containing methanol ultrasonic extraction and alumina impurity removing, such that a near-colorless transparent solution is obtained. The method is simple, fast, reproducible, and accurate. According to the quantitative chromatogram of the three alkaloids, a baseline is stable, peak separation is good, and the peak appearing is completed within 20min. Compared with a traditional method with a buffer-salt solution, a specific chromatographic column, and unconventional organic phase ratio and flow rate, with the method provided by the invention, instrument and chromatographic column service lives are prolonged, cost is reduced, and detection conditions are communized. The method is suitable for popularization and application of basic units.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and relates to a method for detecting the quality of ephedra medicinal materials and preparations thereof, in particular to a content determination method of three alkaloids in ephedra medicinal materials and preparations thereof. Background technique [0002] Ephedra is the dry herbaceous stem of Ephedra sinica Stapf, Ephedra intermedia Schrenk et C.A. Mey. or Ephedra equisetina Bge. It is a commonly used traditional Chinese medicine in a cough compound preparation. It has the effects of sweating and dispelling cold, clearing the lungs and relieving asthma, diuresis and swelling. For anemofrigid cold, chest tightness, dyspnea and cough, feng shui edema. Honey ephedra nourishes the lungs and relieves cough. Preparations refer to Maxing cough tablets and Jin Maxing cough tablets, both of which are compound preparations for cough relief. The prescription, preparati...

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Problems solved by technology

Moreover, the mobile phases of these medicinal materials and preparations are all phosphate buffered salts that are not recommended under (3) of an appendix VI D of the Chinese Pharmacopoeia version in 2010, and the organic phase ratio is mostly lower than 5%. The service life of the column has brought harm, and it also increases the operation steps for the flushing of the chromatographic column after the measurement is completed.

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