Collagen-tea polyphenols antibacterial film and preparation method thereof

A tea polyphenol antibacterial film, collagen technology, applied in biochemical equipment and methods, flexible coverings, packaging and other directions, can solve the problems of limited shelf life, waste of time and energy, long time consumption, etc. Extended shelf life, time and effort saving effect

Inactive Publication Date: 2017-09-19
SHENZHEN INST OF ADVANCED TECH
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, collagen does not have antibacterial activity, and it does not have the effect of inhibiting or killing the microorganisms polluted on the food surface during processing, storage and handling, so it has limitations in the length of food shelf life
[0003] In addition, in the prior art, in the process of preparing the packaging film, the finished packaging film material is generally prepared first, and then the finished material is measured by the Oxford cup method to measure the antibacterial zone against Escherichia coli or Staphylococcus aureus to test the antibacterial properties of the finished material. Effect, if the antibacterial result obtained by the test is not good, it is necessary to adjust the concentration of the antibacterial agent to prepare the packaging film again, and then test the antibacterial effect until the finished material of the packaging film with good antibacterial effect is obtained. This preparation method lacks scientific guidance. The preparation method Complicated and time-consuming, wasting a lot of time and effort

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen-tea polyphenols antibacterial film and preparation method thereof
  • Collagen-tea polyphenols antibacterial film and preparation method thereof
  • Collagen-tea polyphenols antibacterial film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The first aspect of the present invention provides a kind of preparation method of collagen-tea polyphenol antibacterial membrane, comprises the following steps:

[0033] Take the modified collagen solution, dissolve tea polyphenols of different qualities in the modified collagen solution respectively, and stir evenly to obtain collagen-tea polyphenol blends with different concentrations of tea polyphenols;

[0034] The minimum inhibitory concentration test was carried out on the collagen-tea polyphenol blend to obtain the minimum inhibitory concentration of tea polyphenols;

[0035] The minimum bactericidal concentration test was carried out with the collagen-tea polyphenol blend solution having a minimum inhibitory concentration and a concentration greater than the minimum inhibitory concentration, to obtain the minimum bactericidal concentration of tea polyphenols;

[0036] Collagen-tea polyphenol blend liquid with the minimum bactericidal concentration and concentra...

Embodiment 1

[0078] refer to figure 1 A schematic flow chart of the preparation method, a preparation method of collagen-tea polyphenol antibacterial film, comprising the following steps:

[0079] (1) Thermal modification of collagen: Weigh 5g of fish scale collagen and dissolve it in 95mL of distilled water, the thermal modification temperature is 20°C, and the thermal modification time is 30min to obtain a modified collagen solution;

[0080] (2) The minimum inhibitory concentration test of tea polyphenols: take a certain amount of tea polyphenols, dissolve them in the modified collagen solution respectively, the stirring speed is 2000r / min, and obtain the final concentrations of tea polyphenols as 10, 5, Collagen-tea polyphenol blends at 2.5, 1.25, 0.625, 0.313, 0.156, 0.078, 0.039, 0.02 and 0.01 mg / mL;

[0081] Take a sterile 96-well cell culture plate, and add collagen-tea polyphenol blends with tea polyphenol concentrations of 10, 5, 2.5, 1.25, 0.625, 0.313, 0.156, 0.078, 0.039, 0.0...

Embodiment 2

[0087] A preparation method of collagen-tea polyphenol antibacterial film, comprising the following steps:

[0088] (1) Thermal modification of collagen: Weigh 3g of fish scale collagen and dissolve it in 97mL of distilled water, the thermal modification temperature is 60°C, and the thermal modification time is 5min to obtain a modified collagen solution;

[0089] (2) with the step (2) of embodiment 1;

[0090] (3) with the step (3) of embodiment 1;

[0091] (4) Vacuum degassing: Cool the collagen-tea polyphenol blend solution with a tea polyphenol concentration of 0.156mg / mL to room temperature (23°C), degas for 3min in a vacuum of 0.09Mpa, repeat three times, and then statically Set 1h;

[0092] (5) Casting film formation: the collagen-tea polyphenol blend solution was fixed to 100mL, that is, the collagen-tea polyphenol blend solution with a tea polyphenol concentration of 0.156mg / mL after 100mL vacuum degassing was cast to On the plexiglass plate, dry at 60°C for 4 hour...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a preparation method of a collagen-tea polyphenols antibacterial film. The method comprises the following steps: taking modified collagen solutions, respectively dissolving tea polyphenols having different qualities into the modified collagen solutions, and uniformly stirring to obtain collagen-tea polyphenols blending solutions with different concentrations; testing minimal inhibitory concentrations of the collagen-tea polyphenols blending solutions to obtain the minimal inhibitory concentrations of the tea polyphenols; testing minimum bactericidal concentrations of the collagen-tea polyphenols blending solutions which have the minimum inhibitory concentrations and of which the concentrations are more than the minimum inhibitory concentrations to obtain the minimum bactericidal concentrations of the tea polyphenols; carrying out vacuum degassing on the collagen-tea polyphenols blending solutions which have the minimum bactericidal concentrations and of which the concentrations are close to the minimum bactericidal concentrations, casting into a film, and removing the film to obtain the collagen-tea polyphenols antibacterial film. After the minimum inhibitory concentrations and the minimum bactericidal concentrations are obtained by using the preparation method disclosed by the invention, the preparation of the antibacterial film is guided; therefore, the method is simple and feasible.

Description

technical field [0001] The invention relates to the field of food packaging materials, in particular to a collagen-tea polyphenol antibacterial film and a preparation method thereof. Background technique [0002] Edible packaging film has received widespread attention because it can reduce the loss of flavor substances and the consumption of nutrients in food, prevent microbial infection, and effectively prolong food storage and sales. Among the many edible packaging film carriers, collagen is favored because of its safety, non-toxicity, rich nutrition, good film-forming properties and gas barrier properties. However, collagen does not have antibacterial activity, and does not have the effect of inhibiting or killing the microorganisms polluted on the food surface during processing, storage and handling, so it has limitations in the length of food shelf life. [0003] In addition, in the prior art, in the process of preparing the packaging film, the finished packaging film ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00C08K5/1545C12Q1/18B65D65/46
CPCB65D65/463B65D65/466C08J5/18C08J2389/00C08K5/1545C08L2201/06C08L2203/16C12Q1/18C08L89/00
Inventor 王喆叶忱王怀雨
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap