Polysaccharide extract of Chinese caterpillar fungi as well as preparation method and application of polysaccharide extract

A technology for extracting polysaccharides and polysaccharides from Cordyceps militaris is applied in the biological field to achieve the effects of strong purpose, precise components and good effects

Active Publication Date: 2015-04-08
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant literature report on the research on the blood lipid-lowering effect of Cordyceps militaris liquid submerged fermentation

Method used

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  • Polysaccharide extract of Chinese caterpillar fungi as well as preparation method and application of polysaccharide extract
  • Polysaccharide extract of Chinese caterpillar fungi as well as preparation method and application of polysaccharide extract
  • Polysaccharide extract of Chinese caterpillar fungi as well as preparation method and application of polysaccharide extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Cordyceps militaris (C.militaris) obtains the fermentation liquid through slant or plate culture, liquid shaking culture and liquid submerged fermentation culture, and the specific methods are as follows:

[0056] ①Slant or plate strain culture: put the activated mycelium block of the slant parent species of Cordyceps militaris into a new slant solid medium, culture at 24°C for 10 days, and set aside;

[0057] ②First-level liquid strain cultivation: Take 200mL liquid fermentation medium and put it into a 500mL conical flask, sterilize at 121°C for 20 minutes, insert 4 to 6 soybean-sized mycelia blocks of the mother species of Cordyceps militaris; let it stand at room temperature for 24 hours, and wait for After the wound of the bacterial block was healed, it was shaken and cultured at 24°C and 150 rpm in the dark for 6 days to obtain the first-grade seed liquid;

[0058] ③Cultivation of secondary liquid strains: 50L seed tanks are filled with 35L of liquid fermentat...

Embodiment 2

[0066] (1) Cordyceps militaris (C.militaris) obtains the fermentation liquid through slant or plate culture, liquid shaking culture and liquid submerged fermentation culture, and the specific methods are as follows:

[0067] ①Slant or plate strain culture: insert the mycelial block of the activated Cordyceps militaris slant mother species into a new slant solid medium, culture at 20°C for 15 days, and set aside;

[0068] ②First-level liquid strain cultivation: Take 200mL liquid fermentation medium and put it into a 500mL conical flask, sterilize at 121°C for 20 minutes, insert 4 to 6 soybean-sized mycelia blocks of the mother species of Cordyceps militaris; let it stand at room temperature for 24 hours, and wait for After the wounds of the bacteria block were healed, they were shaken and cultured at 24°C and at a speed of 150rpm for 6 days in the dark and at a constant temperature to obtain a first-class liquid strain;

[0069] ③Cultivation of secondary liquid strains: 50L see...

Embodiment 3

[0077] (1) Cordyceps militaris (C.militaris) obtains the fermentation liquid through slant or plate culture, liquid shaking culture and liquid submerged fermentation culture, and the specific methods are as follows:

[0078] ①Slant or plate strain culture: insert the mycelial block of the activated Cordyceps militaris slant mother species into a new slant solid medium, culture at 25°C for 8 days, and set aside;

[0079] ②First-level liquid strain cultivation: Take 200mL liquid fermentation medium and put it into a 500mL conical flask, sterilize at 121°C for 20 minutes, insert 4 to 6 soybean-sized mycelia blocks of the mother species of Cordyceps militaris; let it stand at room temperature for 24 hours, and wait for After the wounds of the bacteria block were healed, they were shaken and cultured at 24°C and at a speed of 150rpm for 6 days in the dark and at a constant temperature to obtain a first-class liquid strain;

[0080] ③Cultivation of secondary liquid strains: 50L seed...

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Abstract

The invention belongs to the field of biotechnology and in particular relates to a polysaccharide extract of Chinese caterpillar fungi as well as a preparation method and application of the polysaccharide extract. The preparation method of the polysaccharide extract of the Chinese caterpillar fungi comprises the following steps: after submerged fermentation culture of mycelium of the Chinese caterpillar fungi, removing the mycelium of a fermented product by virtue of centrifugal separation to obtain a fermentation broth; after removing small molecule substances of the fermentation broth by virtue of ultrafiltration, concentrating the fermentation broth; and carrying out treatments of protein removal, ethanol precipitation, decoloring by hydrogen peroxide, dialysis and vacuum freeze-drying on the concentrated fermentation broth to prepare the polysaccharide extract of the Chinese caterpillar fungi. The preparation method is simple in process and is capable of carrying out efficient mass production with high quality. The prepared polysaccharide extract of the Chinese caterpillar fungi is nonhazardous to a human body, has good physiological function of reducing blood fat and can be used for developing a medicament or health food with the capability of reducing blood fat.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polysaccharide extract of Cordyceps militaris and its preparation method and application. Background technique [0002] Fermentation products are obtained by liquid submerged fermentation of edible and medicinal fungal mycelia, and the intracellular polysaccharides in the mycelium and the exopolysaccharides in the fermentation broth can generally be extracted separately. [0003] The active substances produced by the culture of Cordyceps militaris mycelia are similar to their fruiting bodies in terms of active ingredients, pharmacology, toxicology, clinical and safety (Zeng Hongbin et al., 2011). It is generally believed that Cordyceps polysaccharides, cordycepin, and cordycepic acid are important functional components of Cordyceps militaris (Zhang Rentang et al., 2014). The liquid fermentation of Cordyceps militaris has become the main method to rapidly obtain its myc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/06A23L1/09A23L29/30
Inventor 张松张命龙
Owner SOUTH CHINA NORMAL UNIVERSITY
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