10 results about "Chelirubine" patented technology

The invention provides application of a macleaya cordata extract as a plant source algaecide in cyanobacterial bloom treatment, and relates to the technical field of plant source algaecide product development and environmental protection. The total alkali content of the macleaya cordata extract is 50-80%, and the main effective components of the macleaya cordata extract comprise 40-60% of sanguinarine and 10-20% of chelerythrine. The blue-green algae comprise microcystis aeruginosa, anabaena, nematonema, synechocystis, oscillatoria and nostoc. As a plant source in China, the macleaya cordata has great development and utilization values, has the advantages of wide antibacterial spectrum, no drug resistance, no residue, good safety, wide distribution and the like, and has great development prospects.

The invention discloses a botanical alkaloid and trichoderma synergistic preparation and application thereof, and belongs to the technical field of biological control. The invention provides a preparation containing trichoderma haworthia and chelerythrine in order to solve the technical problems of long period, low prevention and treatment efficiency and easiness in causing harm to environment and human health in a tobacco mosaic disease prevention and treatment method in the prior art. According to the preparation provided by the invention, under the condition that the dilution multiple is 50, the control effect on the tobacco mosaic virus is improved through multi-mechanism synergism, the provided preparation can be applied to tobacco planting production, the control efficiency of the tobacco mosaic disease can be effectively improved, and the economic benefit of tobacco planting is improved.

The invention discloses a method for separating and purifying Sichuan pepper alkaloid based on macroporous adsorption resin and determining the content of the Sichuan pepper alkaloid. The method comprises the steps of extracting, separating and purifying the Sichuan pepper alkaloid, determining the content and formulating the quality standard of the Sichuan pepper alkaloid. NKA-9 type macroporous adsorption resin is adopted to effectively separate and enrich pepper alkaloid, and resin pretreatment conditions, an extraction process and elution conditions (the ethanol concentration is 40%-95%, the solid-liquid ratio is 1: 10-1: 30, the extraction temperature is 45-75 DEG C, and the pH is 2.5-5.5) are optimized, so that the purity and the recovery rate of alkaloid are remarkably improved. According to the present invention, the quality standard of the Sichuan pepper alkaloid is established by using the HPLC method, and the content determination methods of sinomenine, ethyoxyl chelerythrine, chelerythrine and rutaecarpin are set, such that the stability, the repeatability and the high recovery rate of the Sichuan pepper alkaloid are ensured. Precision, stability and sample recovery rate experiments prove that the method disclosed by the invention has good stability and repeatability and is suitable for industrial extraction and application of the pepper alkaloid.

The application discloses an AS1411 aptamer functionalized liposome which comprises lipids, indocyanine green, chelidonium red base and AS1411 aptamer. The application also discloses a preparation method and application of the AS1411 aptamer functionalized liposome. The AS1411 aptamer functionalized liposome provided by the application has excellent fluorescence imaging and chemical-photothermal treatment effects, and is expected to become an ideal diagnosis and treatment nano system and has the potential for future clinical application in cancer treatment.

The application discloses application of chelerythrine combined with polymyxin E in inhibition of polymyxin E drug-resistant bacteria, and the polymyxin E drug-resistant bacteria are mcr positive bacteria. Experiments prove that chelerythrine can inhibit (such as mcr-1 positive escherichia coli ZJ807, mcr-8 positive klebsiella pneumoniae KP91 and mcr-9 positive salmonella SLM183) in cooperation with polymyxin E, and can reduce the use amount of polymyxin E and reverse the drug resistance to polymyxin E. Chelerythrine can restore the inhibitory effect of polymyxin E on the polymyxin E drug-resistant bacteria. The application has important application value.

The invention discloses application of chelerythrine in preparation of drugs or antibiotic synergists for treating bacterial infectious diseases, bacteria are carbapenem antibiotic resistant bacteria, and antibiotics are carbapenem antibiotics. The invention discloses that chelerythrine can restore the sensitivity of carbapenem drug-resistant bacteria for the first time, can reduce the minimum inhibitory concentration of meropenem to bacteria for producing metal beta-lactamase by 16 times, and provides a new strategy for solving the drug resistance of bacteria.

The present invention discloses a 6-(N-substituted)carbamoylmethyldihydrobenzophenanthridine alkaloid and its preparation method and application, relating to the technical field of pharmaceutical compound synthesis. Such compounds are prepared by the following method: using chelerythrine or sanguinarine as raw materials, first undergoing a nucleophilic substitution reaction with ethyl acetate to obtain product A, i.e., 6-ethoxycarbonylmethyldihydrochelerythrine or 6-ethoxycarbonylmethyldihydrosanguinarine; hydrolyzing the ester group of product A under alkaline conditions and acidifying to obtain the corresponding product B, i.e., α-(dihydrochelerythrine 6-base) substituted acetic acid or α-(dihydrosanguinarine 6-base) substituted acetic acid; dissolving product B in a solvent, and condensing with different substituted primary amines under the action of a condensing agent to obtain 6-(N-substituted)carbamoylmethyldihydrochelerythrine or 6-(N-substituted)carbamoylmethyldihydrosanguinarine, that is, This invention uses chelerythrine or sanguinarine as raw materials and introduces a (N-substituted) carbamoylmethyl group at position 6 for structural modification. This series of derivatives, with high yield and ease of preparation, significantly inhibits LPS-induced release of the inflammatory mediator NO and expression of the inflammatory cytokines TNF-α and IL-6 in RAW264.7 cells.

The invention discloses an AS1411 aptamer functionalized liposome. The AS1411 aptamer functionalized liposome comprises a lipid, indocyanine green, chelerythrine and an AS1411 aptamer. The invention also discloses a preparation method and an application of the AS1411 aptamer functionalized liposome. The AS1411 aptamer functionalized liposome loaded with ICG and chelerythrine provided by the invention has excellent fluorescence imaging and chemical-photo-thermal treatment effects, is expected to become an ideal diagnosis and treatment nano system, and has the potential of being clinically applied to cancer treatment in the future.

This invention relates to the field of bactericide technology, specifically to a celandine bactericide and its preparation method. The bactericide is composed of the following parts by weight: antibacterial active ingredient: 0.1-0.5 parts; functional microbial agent: 2-20 parts; carrier: 70-90 parts; wherein the antibacterial active ingredient is composed of celandine and N-aryl-3,4-dihydroisoquinoline derivative in a mass ratio of 1:(0.2-5). This application introduces iodine and bromohalogen atoms at specific positions of N-aryl-3,4-dihydroisoquinoline, enabling it to produce a significant synergistic antibacterial effect with celandine. Beneficial bacteria such as Bacillus amyloliquefaciens and Pseudomonas fluorescens can resist the effects of sub-inhibitory drug concentrations by secreting cyclic lipopeptides and upregulating efflux pumps. The technology of encapsulating the antibacterial active ingredient into a sustained-release unit and combining it with a carrier that adsorbs live bacteria significantly improves the retention rate of live bacteria, achieving a long-term balance between stable drug release and beneficial bacterial activity.

The invention discloses application of chelerythrine and derivatives thereof in prevention and treatment of tobacco mosaic diseases, and belongs to the technical field of plant disease prevention and treatment. The invention provides application of chelerythrine and derivatives thereof in prevention and treatment of tobacco mosaic diseases in order to solve the problem of lack of effective prevention and treatment measures for plants infected with tobacco mosaic viruses in the prior art, and respectively limits the application concentration of chelerythrine and derivatives thereof in prevention and treatment of tobacco mosaic diseases. And the chelerythrine and the derivative dihydrochelerythrine thereof are used as effective components to prepare a compound pesticide preparation. The compound pesticide preparation provided by the invention is relatively low in biotoxicity, relatively high in safety and free of phytotoxicity; the influence on the growth state of the treated tobacco plants is small, the treated tobacco plants have no obvious inhibition or poor growth phenomenon, and the method can be applied to agricultural biological prevention and treatment of tobacco mosaic diseases.