44 results about "Chelirubine" patented technology

The present invention is new total plumepoppy alkaloid salt and its preparation and application. The total plumepoppy alkaloid salts of organic acid and boric acid have the content of 50-90 % accounting in the organic acid salt and boric acid salt of sanguinarine and chelirubine. The total plumepoppy alkaloid salts of organic acid and boric acid have the antibacterial and antiphlogistic activity superior to that of total plumepoppy alkaloid sulfate and hydrochloride. The present invention also relates to the antibacterial and antiphlogistic application of medicines and daily use articles containing total plumepoppy alkaloid salts of organic acid and boric acid for human and animal.

A highly efficient method for catalyzing the synthesis of sanguinarine and chelerythrine, comprising: from the known protopine-6-hydroxylase gene, dihydrobenzophenanthridine oxidase gene and cytochrome P450 reductase gene, by means of heterologous expression and result comparison and analysis, screening out an optimal gene having high expression efficiency, then performing codon optimization on the selected optimal gene; constructing the optimal gene sequence on an expression vector, then transferring same into yeast engineering bacteria for transformation so as to obtain recombinant yeast engineering strains; finally, feeding the recombinant yeast engineering bacteria by using a leaf raw material liquid precursor of Macleaya cordata for fermentation, so as to obtain a product. The invention improves the enzyme catalytic efficiency of sanguinarine and chelerythrine in various aspects such as gene level and fermentation process; the leaf raw material liquid, which is not a traditional medicinal part of Macleaya cordata, is directly used for fermentation with the engineering bacteria, and protopine and allocryptine having high alkaloid content in leaves are converted into high-value sanguinarine and chelerythrine to achieve the comprehensive utilization of Macleaya cordata resources.

The invention discloses a 5-imino substituted derivative with insecticidal and antibacterial properties. The 5-imino substituted derivative is a multi-substituted 1H-1,2,4-triazole amidine compound. This application reports for the first time the application of 5-imino substituted dihydrosanguinarine or chelerythrine derivatives in the field of agrochemicals, obtaining 5-imino substituted dihydrosanginarine or chelerythrine derivatives The compound has the characteristics of high activity, convenient synthesis method, etc., and the derivative also has good insecticidal activity and antibacterial activity, which enriches the structure of bolognagurine analogues and their application in the field of agricultural chemicals.

The invention provides a composition for preventing and treating respiratory diseases of livestock and poultry and a preparation method thereof. The composition includes the following components in mass percentage: 50-90% of the extract of Boluohui leaves; 5%-30% of the extract of Coptidis Rhizoma; Dandelion extract 5% to 30%; Boluohui leaf extract: the mass percentage content of protopine is not less than 0.1%, the mass percentage content of allocryptine is not less than 0.1%, sanguinarine and chelidonine The sum of the mass percentages of kalethrine is not less than 0.2%. The present invention also provides a preparation method of the above composition. The pharmaceutical composition provided by the invention can be used to prevent and treat respiratory diseases of livestock and poultry, promote faster recovery of livestock and poultry, and effectively reduce the mortality rate of sick livestock and poultry.

A chelerythrine suspension agent and a preparation method and application thereof belong to the technical field of vegetable biological pesticides. In order to find an environmentally friendly, low-toxic and efficient control method for rice flax spot disease, the celandine original drug, dispersant Atlox49134%, wetting agent GenapolX0802%, glycerol 5%, magnesium aluminum silicate 0.5%, Xanthan gum 0.1%, Sag15220.2% and deionized water are compounded, and a suspending agent with fine particle size, high suspension rate and no dust is obtained by mixing, shearing and grinding. The suspending agent is used in the prevention and treatment of rice flax spot disease, showing good control effect, and has the advantages of high efficiency, low toxicity, no pollution, easy degradation and no environmental pollution, which is in line with the development of green food and sustainable agricultural development. need.

The present invention provides a macleaya cordata protopine-6-hydroxylase gene optimization sequence and an application thereof, and specifically discloses an optimization sequence MC11229opt obtainedby codon optimization of a protopine-6-hydroxylase gene MC11229, a recombinant expression vector containing the optimization sequence MC11229opt, an engineered yeast of the recombinant expression vector containing the optimization sequence MC11229opt, and applications thereof in preparing dihydrosanguinarine / sanguinarine. An optimization sequence obtained by codon optimization of a functional gene MC11229 of macleaya cordata participating in synthesizing the sanguinarine and chelerythrine according to yeast preferred codons is integrated into saccharomyces cerevisiae for heterologous expression to realize microbial transformation of the sanguinarine and chelerythrine, and compared with non-optimization functional genes, the optimization sequence can greatly improve enzyme catalytic efficiency, improves content of catalytic products of the dihydrosanguinarine and sanguinarine, and reduces production costs of the dihydrosanguinarine and sanguinarine.

The invention discloses flavoprotein oxidase genes of macleaya cordata in synthesis of sanguinarine and chelerythrine and application of the flavoprotein oxidase genes. Three flavoprotein oxidase genes joining in synthesis of sanguinarine and chelerythrine are found in a macleaya cordata genome for the first time, including genes Mc20113, Mc6407 and Mc6408. Steps of reaction are respectively verified by using a brewer's yeast system through upstream precursor feeding, and synthesis of sanguinarine and chelerythrine and midbodies can be achieved. The conversion efficiency of same functional genes of macleaya cordata and opium poppy is further compared by using a brewer's yeast heterologous expression system, and the result shows that the conversion rate of the gene of macleaya cordata is remarkably higher than that of opium poppy. The invention further discloses a molecular mechanism of synthesis of sanguinarine in macleaya cordata, and a theoretic basis and molecular assistant breeding targets are provided for breeding of macleaya cordata with high contents of sanguinarine and chelerythrine; and meanwhile, precious experience is provided for in-vitro artificial synthesis of sanguinarine and chelerythrine.

The invention discloses a making method of polyphenylacetylene typed adsorption resin with high-polarity group and application to bocconine alkaloid, which is characterized by the following: synthesizing new-typed adsorption resin based on polyphenylacetylene as frame with high-polarity group content according to structural feature of sanguinarine and toddaline; utilizing the synergic effect of resin dipole action and hydrophobic action; separating sanguinarine and toddaline from resin.

The invention discloses the application of an antibacterial traditional Chinese medicine component composition in killing biofilms. The invention adopts the in vitro model of biofilms to study and finds that the mixture of chelerythrine, sanguinarine and berberine is effective against Candida albicans and In vitro intervention of Streptococcus mutans dual-species biofilm, the mixture can significantly inhibit and clear the dual-species biofilm of Candida albicans and Streptococcus mutans.

The invention provides an optimized sequence of a macleaya cordata dihydrobenzophenanthridine oxidase gene and application thereof. The invention comprises codon optimization on a dihydrobenzophenanthridine oxidase gene MC6408 to obtain an optimized sequence MC6408opt, a recombinant expression vector of the optimized sequence MC6408opt, yeast engineering bacteria containing the recombinant expression vector of the optimized sequence MC6408opt, and application of the optimized sequence MC6408opt, recombinant expression vector and yeast engineering bacteria in the preparation of pseudochelerythrine. The functional gene MC6408 of macleaya cordata participating in synthesis of pseudochelerythrine and chelerythrine is subjected to codon optimization according to a yeast preferred codon to obtain the optimized sequence; and the optimized sequence is integrated into saccharomyces cerevisiae for heterologous expression, so that microbial transformation of the pseudochelerythrine and chelerythrine is realized. Compared with an unoptimized functional gene, the optimized gene can greatly improve the enzymatic efficiency of the dihydrobenzophenanthridine oxidase, increase the content of the catalytic product pseudochelerythrine, and reduce the production cost of the pseudochelerythrine.