Chelerythrine mPEG-PLGA nanogel composite preparation as well as preparation method, detection method and application of chelerythrine mPEG-PLGA nanogel composite preparation

Abstract

The invention relates to a chelerythrine mPEG-PLGA nanogel composite preparation as well as a preparation method, a detection method and application thereof, chelerythrine nanoparticles taking mPEG-PLGA as a carrier are successfully prepared by adopting a nanoprecipitation method, the chelerythrine nanoparticles are prepared into the temperature-sensitive nanogel composite preparation, and the targeting effect of chelerythrine on tumors is improved. The solid tumor inhibitor has relatively high encapsulation efficiency, drug loading capacity, biological safety and slow release effect, can effectively inhibit the growth of solid tumors and the tumor inhibition action mechanism thereof, provides a theoretical basis for further clinical application, and has application prospects in the aspect of treatment of melanoma; and a basis and a reference can be provided for constructing a safe, stable and intelligent hydrophobic anti-cancer drug carrier.

Description

technical field [0001] The invention belongs to the technical field of drug development and analysis, and in particular relates to a chelerythrine mPEG-PLGA nano gel composite preparation and its preparation, detection method and application. Background technique [0002] Chelerythrine (Chelerythrine, CHE) is an alkaloid active ingredient derived from medicinal plants such as Macleaya cordata (Willd.) R.Br. and Chelidonium majus Linn. . A large number of research data at home and abroad show that CHE can inhibit tumor growth, such as human colon cancer HCT-116, human liver cancer cells HepG 2, melanoma B16 cells, etc. However, CHE has poor water solubility, and it is difficult for free drugs to diffuse and penetrate in tumor sites, which limits its clinical development and utilization. [0003] Article ID: 2095-817X(2018)04-0041-004, "Research on the preparation of chelerythrine polybutylcyanoacrylate nanoparticles", Cao Xu, Zhao Lei, Sun Chen. It is disclosed that poly(n...

AI Technical Summary

Problems solved by technology

[0006] The technical problem existing in the application number CN201510254971.3 and the difference from the technology of the present application is that the magnetic nanoparticles in this patent have a good targeting effect, but due to the high surface effect of the magnetic nanoparticles, they are very Aggregation reactions may occur, limiting the range of applications of magnetic nanoparticles

At the same time, some magnetic nanoparticles also reach other tissues in the body, which may cause toxic side effects

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