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High polarity polystyrene adsorption resin preparation method and its uses in separating bocconine

A technology of Boluohui biology and adsorption resin, which is applied in the direction of chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of high cost, complicated operation, separation, etc., and achieve low production cost and high process efficiency. Simple, Environmentally Friendly Effects

Inactive Publication Date: 2008-05-28
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above two chromatographic methods are costly and complicated to operate, and are not suitable for industrial production
At present, there are reports in the literature that adsorption resins are used to extract the total alkaloids of boluohui, but the resins used are still all polystyrene or adsorption resins containing a small amount of weak polar groups, and the hydrophobic force of this type of resin is very strong. , has the same adsorption capacity for sanguinarine and chelerythrine, which have most of the same hydrophobic structure, so the two cannot be effectively separated
At present, there is no relevant report on the separation of sanguinarine and chelerythrine by resin method

Method used

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  • High polarity polystyrene adsorption resin preparation method and its uses in separating bocconine

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Effect test

Embodiment 1

[0024] The preparation method of the polystyrene type adsorption resin of high polar group content is realized through the following steps:

[0025] At room temperature, dissolve gelatin or polyvinyl alcohol in water to make a 1% aqueous solution, heat it to 40°C, add a reaction monomer consisting of 50% methyl acrylate, 10% styrene and 40% divinylbenzene, Add AIBN with 1% of the monomer amount as the initiator, add long-chain alkanes as the porogen, and its amount is 85% of the monomer amount, mix the above-mentioned raw materials to obtain the oil phase of the reaction, and adjust the volume of the water phase: oil phase Ratio = 3:1; Slowly raise the temperature to 70°C for more than 4 hours, then slowly raise the temperature to 90°C for more than 3 hours, after filtration and washing, the required adsorption resin is prepared.

Embodiment 2

[0027] Application of the above-mentioned adsorption resin in the separation of sanguinarine and chelerythrine contained in Boluohui

[0028] About 40% sanguinarine content, about 20% chelerythrine content of the commercially available Boluohui extract 13.5mg, dissolved in 30ml 6% ethanol aqueous solution, made into the upper column liquid, through the above-mentioned The resin column of resin (column diameter 15mm, 30ml wet resin is housed), adsorption speed is 0.5BV / h, after adsorption is finished, step-elution with 40% ethanol aqueous solution and 70% ethanol aqueous solution, flow rate is 1BV / h, The two parts of the eluate were distilled under reduced pressure and dried in vacuum to obtain solids. The contents of chelerythrine and sanguinarine were 96.2% and 92.3%, respectively, as detected by Waters 484 high-performance liquid chromatography.

Embodiment 3

[0030] About 40% of sanguinarine content, 13.1 mg of commercially available bolohui extract with about 20% of chelerythrine content are dissolved in 30ml of 6% ethanol aqueous solution to make upper column liquid, and the above-mentioned The resin column of resin (column diameter 15mm, 30ml wet resin is housed), adsorption speed is 1.5BV / h, after adsorption is completed, step-elution with 40% ethanol aqueous solution and 70% ethanol aqueous solution, flow rate is 1BV / h, The two parts of the eluate were distilled under reduced pressure and dried in vacuum respectively to obtain solids. The contents of chelerythrine and sanguinarine were 95.2% and 93.2% respectively after detection by Waters 484 high performance liquid chromatography.

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Abstract

The invention discloses a making method of polyphenylacetylene typed adsorption resin with high-polarity group and application to bocconine alkaloid, which is characterized by the following: synthesizing new-typed adsorption resin based on polyphenylacetylene as frame with high-polarity group content according to structural feature of sanguinarine and toddaline; utilizing the synergic effect of resin dipole action and hydrophobic action; separating sanguinarine and toddaline from resin.

Description

【Technical field】 [0001] The invention relates to the technical field of extraction and separation of active ingredients of traditional Chinese medicines, in particular to a preparation method of a highly polar polystyrene adsorption resin and the application of sanguinarine and chelerythrine contained in the separation of boluohui. 【Background technique】 [0002] Macleaya cordata is a plant of the family Poppyaceae, also known as trumpet pole and mountain horn, a perennial herb that is widely distributed in my country. It has a long history of being used in the folk for treating scabies, furunculosis, and killing insects and maggots. The main functions of Boluohui are detoxification, detumescence, insecticide, etc. It is mainly used in the treatment of hepatitis B, finger boils, abscess, acute tonsillitis, etc. in medicine. The main active ingredients are isoquinoline derivatives sanguinarine and chelerythrine, and also contain a small amount of proto-opiate, α-allocryptine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/14C08F2/18C08F4/04B01J20/26C07D455/03A61K36/66C08F212/08C08F212/36
Inventor 王春红施荣富皮国沛俞嘉梅
Owner NANKAI UNIV
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