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Quality control method of Bopu total alkali

A quality control method and sanguinarine technology are applied in the field of quality control of Bopu total alkaloids, which can solve the problems such as no quality control method of Bopu total alkaloids, and achieve the effect of preventing production.

Inactive Publication Date: 2021-07-30
MICOLTA BIORESOURCE INC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned comparative documents provide quality control methods for ginger processed products and Jianghoupu formula granules, but there is no quality control method for Bopu total alkaloids in the prior art, so it is imperative to find a quality method for Bopu total alkaloids

Method used

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  • Quality control method of Bopu total alkali
  • Quality control method of Bopu total alkali
  • Quality control method of Bopu total alkali

Examples

Experimental program
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Effect test

preparation example Construction

[0037] S1, the preparation of test solution

[0038]Take Bopu total alkali powder, accurately weigh it, place it in a stoppered Erlenmeyer flask, accurately add 50ml of methanol-1.0% hydrochloric acid aqueous solution in equal proportions, weigh it, and ultrasonically treat it for 30min under the conditions of power 250W and 33KHz, take it out, Let it cool, weigh it, make up the lost weight with methanol-1.0% hydrochloric acid water, shake well, filter, and take the filtrate to obtain the test solution.

[0039] S2, preparation of reference substance solution

[0040] Take at least one of the original opium, allocryptine, sanguinarine and chelerythrine reference substances, add an equal proportion of methanol-1.0% aqueous hydrochloric acid solution after accurate weighing, and obtain the reference substance solution;

[0041] S3, respectively inject the need testing solution prepared in step S1 and the reference solution prepared in step S2 into a high performance liquid chro...

Embodiment 1

[0062] (1) Preparation of test solution

[0063] Get the Bopu total alkali powder 17.66mg that batch number is 200501, accurately weigh, put in the Erlenmeyer flask with stopper, accurately add methanol-1.0% hydrochloric acid water (50:50) 50ml, weigh, ultrasonic (power 250W, 33KHz ) for 30 minutes, take it out, let it cool, weigh it, make up the lost weight with methanol-1.0% hydrochloric acid water (50:50), shake well, filter, and take the filtrate to obtain the final product.

[0064] (2) Preparation of reference substance solution

[0065] Take 9.51 mg of the original opium reference substance, 4.93 mg of allocryptine, 2.11 mg of sanguinarine hydrochloride, and 1.23 mg of chelerythrine, accurately weighed, add methanol-1.0% hydrochloric acid (50:50) 100ml to dissolve, and prepare Make a solution containing 0.095 mg of pro-opiate, 0.049 mg of allocryptine, 0.021 mg of sanguinarine hydrochloride, and 0.012 mg of chelerythrine per 1 ml.

[0066] (3) Agilent TC-C18 (250×4.6m...

Embodiment 2

[0074] (1) Preparation of test solution

[0075] Get the Bopu total alkali powder 18.91mg that batch number is 200502, accurately weigh, put in the Erlenmeyer flask with stopper, accurately add methanol-1.0% hydrochloric acid water (50:50) 50ml, weigh, ultrasonic (power 250W, 33KHz ) for 30 minutes, take it out, let it cool, weigh it, make up the lost weight with methanol-1.0% hydrochloric acid water (50:50), shake well, filter, and take the filtrate to obtain the final product.

[0076] (2) Preparation of reference substance solution

[0077] Take 9.51mg of the original opioid reference substance, 4.93mg of allocryptine, and 1.23mg of chelerythrine, accurately weighed, add methanol-1.0% hydrochloric acid (50:50) 100ml to dissolve, and make each 1ml containing the original opioid 0.095mg, allocryptine 0.049mg, chelerythrine 0.012mg solution.

[0078] (3) Agilent TC-C18 (250×4.6mm, 5 μm) chromatographic column; acetonitrile is used as mobile phase A, 0.1% phosphoric acid is u...

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Abstract

The invention discloses a quality control method of Bopu total alkali, which adopts a liquid chromatograph, and uses a method of reference substance accompanying control or combination of reference substance accompanying control and relative retention time to qualitatively identify that the Bopu total alkali contains four substances, i.e., protopine, allocryptopine, sanguinarine and chelerythrine. According to the method, the chromatographic characteristic peaks of the liquid chromatogram of a sample are identified through utilizing the characteristic chromatogram of a liquid phase by utilizing reference substance accompanying control and relative retention time of a reference peak, and the result identifies all four characteristic peaks of the Bopu total alkali which are basically free of other impurity peaks. By adopting thequality control method, other medicinal parts can be effectively prevented from being fed, and other chemical medicines can be effectively prevented from being added into the raw material medicine, so that the product quality is controlled.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines, and in particular relates to a quality control method of Bopu total alkaloids. Background technique [0002] Boluohui is a perennial erect herbaceous plant of the genus Poppy Keboluohui. The main chemical components of Boluohui are alkaloids, the highest content in the fruit, and the main alkaloids are sanguinarine, chelerythrine, protopine, and allocryptopine . Early studies mainly extracted and separated benzophenanthridine alkaloids from Boluohui fruit, which were used as an appetizer for livestock and poultry to promote growth. It mainly contained sanguinarine and chelerythrine, and basically did not contain protopine and other hidden substances. alkali. In the process of production and research, in order to comprehensively utilize the resources of Boluohui, the waste filtrate and residue produced by extracting benzophenanthridine total alkaloid sal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686
Inventor 陈燕乐曾建国杨广民胡佑凡
Owner MICOLTA BIORESOURCE INC LTD
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