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Method for preparing chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals

A technology of chelerythrine and aquatic animals, which is applied in the direction of extracellular fluid diseases, blood diseases, antibacterial drugs, etc., can solve the problems of increasing the difficulty of disease prevention, endangering human health, and difficult degradation of chemical drugs, etc. Achieve the effect of rapid treatment of bacterial hemorrhagic disease, less resistance to drugs, and significant control effect

Inactive Publication Date: 2011-05-18
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used prevention and treatment drugs are some oral drugs such as norfloxacin, anthranoxacin, gentamycin sulfate and other chemical synthetic drugs and antibiotics. Due to long-term use, drug resistance occurs, and some chemical drugs are not easy to degrade. , pollute the water environment; these medical raw materials remain and accumulate in aquatic animals, endangering human health, and indirectly lead to drug resistance of human pathogens, making it more difficult for people to prevent and control diseases

Method used

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  • Method for preparing chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals
  • Method for preparing chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals
  • Method for preparing chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals

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Embodiment 1

[0117] Embodiment 1: Preparation method of chelerythrine bacterial disease prevention and treatment agent for aquatic animals

[0118] 1. Extraction and separation of chelerythrine extract (purity 80%)

[0119] 1. Extraction

[0120] Dry and pulverize the Boluohui seeds, use 8-10 times of methanol, extract under reflux at 70°C for 3 times, each time for 2 hours, combine the extracts, filter, concentrate under reduced pressure to obtain extracts, extract with petroleum ether, and extract Concentrate under reduced pressure to obtain extract and separate by silica gel column chromatography.

[0121] 2. Separation

[0122] For large-scale production, the above Boluohui extract is separated by 100-200 mesh silica gel, stainless steel chromatography column, petroleum ether: ethyl acetate: methanol gradient elution. Thin-layer chromatography (TLC) method was used for detection and analysis, and similar fractions were merged, and the obtained six streams were grouped: A, B, C, D,...

Embodiment 2

[0138] The preparation method of embodiment 2 control agent

[0139] The chelerythrine aquatic animal bacterial disease prevention agent is composed of chelerythrine extract (80% purity) and other auxiliary materials. The formulation of the preparation is in the following mass percentages: 20 parts of chelerythrine, 80 parts of carrier starch constitutes 100 parts.

[0140]The preparation method is as follows: weighing 20 g of chelerythrine extract (purity 80%), adding 80 g of starch carrier, and fully mixing and stirring to obtain 100 g of the preparation containing 16% chelerythrine.

Embodiment 3

[0141] The preparation method of embodiment 3 control agent

[0142] The chelerythrine bacterial disease prevention agent for aquatic animals is composed of chelerythrine extract (80% purity) and other auxiliary materials. The formulation of the preparation is in the following mass percentages: 15 parts of chelerythrine, 85 parts of carrier starch constitutes 100 parts.

[0143] The preparation method is as follows: weigh 15 g of chelerythrine extract (purity: 80%), add 85 g of starch carrier, and fully mix and stir to obtain 100 g of the preparation containing 12% chelerythrine.

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Abstract

The invention discloses a method for preparing a chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals. The medicament consists of chelerythrine and starch serving as an auxiliary material. The method for preparing the prevention and treatment agent comprises the following steps of: weighing chelerythrine extract (the purity is 80 percent) and starch serving as the auxiliary material, adding the chelerythrine extract and the starch into a mixer, and mixing the chelerythrine extract and the starch uniformly to obtain a preparation containing 5 to 20 percent chelerythrine. When the bacterial disease prevention and treatment agent is added into feed for feeding, the dosage is 200 to 250 mg / kg (according to the quantity of the chelerythrine for each kilogram of weight); the prevention and treatment agent added in the feed has low toxicity, does not affect the quality of aquatic products, has no residue or pollution, is not easy to generate medicament resistance, does not affect the feed and growth of the aquatic animals, has low dosage, and is widely applied in prevention and treatment of bacterial hemorrhage diseases of mandarin fish, weever, carp, grass carp, eel, porgy, flounder and the like.

Description

technical field [0001] The invention belongs to the new application of chelerythrine, in particular to the preparation of chelerythrine for killing pathogenic bacteria in aquatic animals, such as Flavobacterium columnar, Vibrio anguillarum, Aeromonas punctatus subsp. Aeromonas, Vibrio alginolyticus, Vibrio harveyi, Aeromonas salmonicida and other pathogenic bacteria, especially the application of fish bacterial hemorrhagic disease caused by Aeromonas hydrophila and the Pharmaceutical preparations of clarithrine. Background technique [0002] Bacterial disease is a common disease that seriously harms aquatic animals, especially the bacterial hemorrhagic disease caused by Aeromonas hydrophila, which is the most serious harm to fishery production. At present, the commonly used prevention and treatment drugs are some oral drugs such as norfloxacin, anthranoxacin, gentamycin sulfate and other chemical synthetic drugs and antibiotics. Due to long-term use, drug resistance occurs,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61P31/04A61P7/04
Inventor 王庆吴淑勤石存斌王高学刘春曾伟伟李宁求
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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