Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Optimized Sequence of Boluohui Dihydrotriphenanthridine Oxidase Gene and Its Application

A dihydrotriphenanthridine oxidase and gene sequence technology, which is applied in the field of genetic engineering and bioengineering, can solve problems such as the reporting of unreported P6H gene expression, and achieve the effects of improving enzyme catalytic efficiency, increasing content, and reducing production costs

Active Publication Date: 2020-10-09
MICOLTA BIORESOURCE INC LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no scholars have reported the expression of P6H gene in different species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Optimized Sequence of Boluohui Dihydrotriphenanthridine Oxidase Gene and Its Application
  • Optimized Sequence of Boluohui Dihydrotriphenanthridine Oxidase Gene and Its Application
  • Optimized Sequence of Boluohui Dihydrotriphenanthridine Oxidase Gene and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] In order to better illustrate the content of the invention, the present invention will be further verified by specific examples below. It is specially stated here that the embodiments are only for describing the present invention more directly, they are only a part of the present invention, and cannot constitute any limitation to the present invention.

[0032] as attached figure 1 The synthetic pathways of sanguinarine and chelerythrine in Boluohui shown, the dihydrobenzophenanthridine oxidase (DBOX) gene is involved in the biogenesis of sanguinarine (SAN) and chelerythrine (CHE) Synthetic steps, which can catalyze dihydrochelerythrine (DHCHE) to generate chelerythrine (CHE) and catalyze dihydrosanginarine (DHSAN) to generate sanguinarine (SAN). Dihydrotriphenylene oxidase (DBOX) belongs to the flavoprotein oxidase gene.

[0033] In the applicant's previous research, the flavin protein oxidase-related gene sequence of other species (poppy, poppy) that has been publis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an optimized sequence of a macleaya cordata dihydrobenzophenanthridine oxidase gene and application thereof. The invention comprises codon optimization on a dihydrobenzophenanthridine oxidase gene MC6408 to obtain an optimized sequence MC6408opt, a recombinant expression vector of the optimized sequence MC6408opt, yeast engineering bacteria containing the recombinant expression vector of the optimized sequence MC6408opt, and application of the optimized sequence MC6408opt, recombinant expression vector and yeast engineering bacteria in the preparation of pseudochelerythrine. The functional gene MC6408 of macleaya cordata participating in synthesis of pseudochelerythrine and chelerythrine is subjected to codon optimization according to a yeast preferred codon to obtain the optimized sequence; and the optimized sequence is integrated into saccharomyces cerevisiae for heterologous expression, so that microbial transformation of the pseudochelerythrine and chelerythrine is realized. Compared with an unoptimized functional gene, the optimized gene can greatly improve the enzymatic efficiency of the dihydrobenzophenanthridine oxidase, increase the content of the catalytic product pseudochelerythrine, and reduce the production cost of the pseudochelerythrine.

Description

technical field [0001] The invention relates to an optimized gene sequence of Boluohui dihydrotriphenylene oxidase and its application, belonging to the technical field of genetic engineering and bioengineering. [0002] technical background [0003] Medicinal plants are plants that have the curative effect of preventing and curing diseases in Chinese traditional medicine and are used as the source of medicines. They have a very important position in Chinese medicine. The active ingredients of many medicinal plants are their secondary metabolites, which have attracted widespread attention due to their unique curative effect and low toxicity and side effects. my country is rich in wild medicinal plant resources, but due to the destruction of the natural environment and the over-exploitation and abuse of medicinal plants in recent years, the medicinal plant resources in my country are on the verge of exhaustion. Wild resources can no longer meet people's needs, and there are p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/06C12N15/81C12N1/19C12P17/18C12R1/865
CPCC12N9/0032C12N15/81C12N2800/22C12P17/18C12Y105/03012
Inventor 黄鹏曾建国
Owner MICOLTA BIORESOURCE INC LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com