Application of chelerythrine in inhibition of colistin drug resistance gene transfer

A technology of chelerythrine and drug-resistant genes, applied in the field of biomedicine, can solve problems such as limiting the application of colistin, achieve wide application value, and inhibit the spread of the effect

Pending Publication Date: 2022-07-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

IncI2 and IncX4 plasmids are still the most prevalent plasmid types carrying the mcr-1 gene, and the prevalence of the mcr-1 gene severely limits the clinical application of colistin and poultry farming

Method used

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  • Application of chelerythrine in inhibition of colistin drug resistance gene transfer

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] The preparation method of the culture medium involved in the following examples is as follows:

[0030] BHI broth medium: add 18.5 g of LB broth medium (Beijing Land Bridge Technology Co., Ltd.) to an appropriate amount of distilled water, then dilute to 500 mL with distilled water, sterilize by autoclaving at 121°C for 20 min, and cool.

[0031] BHI agar medium: add 25 g of BHI agar medium (Beijing Luqiao Technology Co., Ltd.) to an appropriate amount of distilled water, then dilute to 500 mL with distilled water, sterilize by autoclaving at 121 °C for 20 min, pour the plate, and cool.

Embodiment 1

[0032] Example 1. Identification of Escherichia coli ZJ807, Escherichia coli ZJ28 and Escherichia coli J53

[0033] Escherichia coli ZJ807 was described in the following documents: Prevalence, risk factors, outcomes, andmolecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study), and the genome sequence was uploaded to NCBI, BioSample Accession Number: SAMN05437814.

[0034] Escherichia coli ZJ28 was described in the following documents: Prevalence, risk factors, outcomes, andmolecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study, and the genome sequence was uploaded to NCBI, BioSample Accession Number: SAMN05437813 .

[0035] Escherichia coli J53 is described in: Genome sequence of Escherichia coli J53, a reference strain for genetic studies. GenBank accession number AICK00000000.

[0036] It was identified t...

Embodiment 2

[0037] Example 2. Application of chelerythrine in inhibiting the transfer of mcr-1 positive plasmids

[0038] Using Escherichia coli ZJ28 or Escherichia coli ZJ807 as the donor strain and Escherichia coli J53 as the recipient strain, the effect of chelerythrine on the transfer of mcr-1 positive plasmids between Escherichia coli was evaluated by conjugation transfer method.

[0039] 1. Preparation of polymyxin E stock solution, chelerythrine stock solution and sodium azide stock solution

[0040] (1) get polymyxin E, dilute with deionized water, obtain the polymyxin E solution with a concentration of 2560 μg / mL; then use the polymyxin E solution with a sterile filter membrane (Pall company in the United States, the pore size is 0.22 μm) to obtain a stock solution of polymyxin E.

[0041] (2) Take chelerythrine and dilute it with deionized water to obtain a chelerythrine solution with a concentration of 1000 μg / mL; then filter the chelerythrine solution with a sterile filter to...

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Abstract

The invention discloses an application of chelerythrine in inhibition of colistin drug resistance gene transfer. A conjugational transfer test proves that chelerythrine can obviously inhibit the transfer of plasmids carrying a colistin drug-resistant gene mcr-1 between escherichia coli. Therefore, the chelerythrine can inhibit propagation of colistin drug-resistant plasmids, provides a new thought and source for control of colistin drug resistance, and has wide application value in the fields of medicine, food, veterinary public health and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the application of chelerythrine in inhibiting the transfer of colistin resistance genes. Background technique [0002] Colistin resistance has attracted much attention in recent years. The main mechanism of colistin resistance is mutation of the binary regulatory system on the chromosome, and the resistance does not spread easily. In 2015, Chinese researchers first reported the plasmid-mediated transferable colistin resistance gene mcr-1 in pig-derived Escherichia coli, revealing the reasons for the increase of colistin resistance in animal-derived bacteria in recent years. At present, it is found that mcr-1 gene mainly exists on IncI2, IncX4 and IncHI2 plasmids of Escherichia coli, and it has been widely spread in more than 60 countries around the world. The IncI2 and IncX4 plasmids are still the most popular plasmids carrying the mcr-1 gene, and the prevalence of the mcr-1 gene se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/70C12N15/09C12N1/20C12R1/19
CPCC12N15/70C12N15/09C12N1/20
Inventor 汪洋吴聪明王雪杨宋黄威张慕琛邹之宇杨思源沈应博刘德俊沈建忠
Owner CHINA AGRI UNIV
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