Application of chelerythrine to preparation of medicine for inhibiting blastomyces albicans and staphylococcus aureus bi-species biofilm

A technology of Candida albicans and chelerythrine, which is applied in the directions of antibacterial drugs, antifungal agents, pharmaceutical formulations, etc., can solve the problems that have not yet been seen.

Inactive Publication Date: 2020-02-21
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chelidonium has a certain inhibitory effect on bacteria and fungi, but there is no report on the application of chelerythrine to the growth of dual-species biofilms of Candida albicans and Staphylococcus aureus

Method used

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  • Application of chelerythrine to preparation of medicine for inhibiting blastomyces albicans and staphylococcus aureus bi-species biofilm
  • Application of chelerythrine to preparation of medicine for inhibiting blastomyces albicans and staphylococcus aureus bi-species biofilm
  • Application of chelerythrine to preparation of medicine for inhibiting blastomyces albicans and staphylococcus aureus bi-species biofilm

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with the examples. The examples are only used to explain the content of the present invention, not to limit the present invention.

[0022] 1. Materials

[0023] 1.1 Experimental strains

[0024] Candida albicans SC5314 was purchased from the American Type Culture Collection;

[0025] Staphylococcus aureus ATCC25923 was purchased from the American Type Culture Collection.

[0026] 1.2 Reagents

[0027] Dimethyl sulfoxide DMSO was purchased from Shanghai Sigma-Aldrich Trading Co., Ltd.;

[0028] Normal saline was purchased from Shanghai Sigma-Aldrich Trading Co., Ltd.;

[0029] Methanol was purchased from Shanghai Sigma-Aldrich Trading Co., Ltd.;

[0030] Yeast extract powder was purchased from Shanghai Sigma-Aldrich Trading Co., Ltd.;

[0031] Glucose was purchased from Shanghai Sigma-Aldrich Trading Co., Ltd.;

[0032] Peptone was purchased from Shanghai Sigma-Aldrich Trading Co., Lt...

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Abstract

The invention discloses application of chelerythrine to preparation of a medicine for inhibiting blastomyces albicans and staphylococcus aureus bi-species biofilm. By adopting a blastomyces albicans and staphylococcus aureus bi-species biofilm in vitro model, an in vitro intervention effect of the chelerythrine on the bi-species biofilm is researched; through observation of a crystal violet method, the phenomenon that the chelerythrine can effectively intervene formation of the blastomyces albicans and staphylococcus aureus biofilm is found; and through observation of field emission scanning electron microscope, the phenomenon that the chelerythrine has obvious inhibition and clearing effects on the blastomyces albicans and staphylococcus aureus in the biofilm is found; and therefore, thechelerythrine has definite effects on inhibition of the blastomyces albicans and staphylococcus aureus bi-species biofilm, and can be applied to preparing the medicine for inhibiting or clearing the blastomyces albicans and staphylococcus aureus bi-species biofilm; and therefore, the application of the chelerythrine has an effective meaning.

Description

technical field [0001] The invention belongs to the technical field of medicine and food safety, and relates to the application of chelerythrine in the preparation of a drug for inhibiting Candida albicans and Staphylococcus aureus dual-species biofilm. Background technique [0002] Candida albicans, also known as Candida albicans, is a common oral flora and an important conditional pathogenic fungus in the oral cavity. Under certain conditions, it can cause a variety of oral mucosal diseases. It is one of the most common pathogenic bacteria causing deep fungal infection clinically. Staphylococcus aureus can infect humans and various animals, causing local infection, abscess, cellulitis, and severe sepsis or even death. [0003] In recent years, with the advancement of medical technology, medical implants such as catheters, artificial valves and joint replacements are more likely to be used for clinical treatment. Pathogens adhere to the surface of medical implants to form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61P31/10A61P31/04
CPCA61K31/4741A61P31/04A61P31/10
Inventor 钱卫东张家宁王文静刘淼孙照欢杨敏付玉婷李鑫辰
Owner SHAANXI UNIV OF SCI & TECH
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