Compound for inhibiting strawberry angular leaf spot from growth

A technology of Xanthomonas fragariae and compounds, applied in the field of compounds, can solve economic losses, increase social and economic costs, and affect the import and export trade of strawberries, etc.

Inactive Publication Date: 2015-12-02
许自协
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, it will seriously affect the import and export trade of strawberries in my country, resulting in huge economic losses.
Once the disease occurs, measures such as blockade and quarantine to control its spread will affect the circulation of domestic strawberry seedlings and fruits, increasing the corresponding social and economic costs

Method used

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  • Compound for inhibiting strawberry angular leaf spot from growth
  • Compound for inhibiting strawberry angular leaf spot from growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Measure the minimum inhibitory concentration MIC value.

[0016] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0017] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0018] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0019] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially on...

Embodiment 2

[0026] Measure the minimum bactericidal concentration MBC value.

[0027] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0028] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0029] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0030] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially ...

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PUM

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Abstract

The invention provides a compound with antibacterial and bactericidal activities for strawberry angular leaf spot. The minimal inhibitory concentration (MIC) value for the strawberry angular leaf spot is 8 mu g / ml, and the minimum bactericidal concentration (MBC) value is 32 mu g / ml.

Description

technical field [0001] The invention relates to a new application of the compound, clarifies the relationship between the chemical structure of the medicine and the biological activity of bacteria, and more specifically, explores the bacteriostasis and bactericidal activity of the compound on X. fragariae. Background technique [0002] Xanthomonas fragariae Kennedyet King is an extremely serious bacterial disease in strawberry production, and its pathogen Xanthomonas fragariae Kennedyet King is a quarantine pest for imported plants in my country. In recent years, in order to improve the quality of domestic strawberries and meet the growing demand of the market, my country has successively introduced a large number of high-quality strawberry seedlings from abroad. With the increase of imported strawberry seedlings, the risk of X. fragariae being introduced into my country is increasing, and the threat to my country's growing strawberry industry is also increasing. [0003] S...

Claims

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Application Information

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IPC IPC(8): C07D307/93A01N43/12A01P1/00
CPCA01N43/12C07D307/93
Inventor 不公告发明人
Owner 许自协
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