Compound for inhibiting strawberry angular leaf spot from growth
A technology of Xanthomonas fragariae and compounds, applied in the field of compounds, can solve economic losses, increase social and economic costs, and affect the import and export trade of strawberries, etc.
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Embodiment 1
[0015] Measure the minimum inhibitory concentration MIC value.
[0016] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.
[0017] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.
[0018] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.
[0019] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially on...
Embodiment 2
[0026] Measure the minimum bactericidal concentration MBC value.
[0027] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.
[0028] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.
[0029] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.
[0030] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially ...
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