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Bacillus velezensis and application thereof

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of poor control efficiency, insufficient colonization ability of antagonistic bacteria, and limited control range, etc., to achieve production and transportation Low cost, convenient processing, good control effect

Active Publication Date: 2020-05-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of insufficient colonization ability of antagonistic bacteria, poor control efficiency, and limited control range in the existing bio-control technology, the present invention provides a strain of Bacillus Velez with a bio-control and growth-promoting effect and its application

Method used

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  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation, Purification and Identification of Bacillus Velez

[0022] In October 2017, in Fangcun Town, Daiyue District, Tai'an City, Shandong Province, the soil of greenhouses with better facilities to solve the obstacles of continuous sitting was collected, and bacterial strains were isolated from it. In a 250 mL sterilized Erlenmeyer flask filled with 90 mL of sterile water and a small amount of glass beads, place the Erlenmeyer flask on a shaker at 37 °C and 180 rpm for 30 min to fully suspend it, then treat it in a water bath at 80 °C for 10 min, and use Bacterial water to dilute the bacterial suspension to 10 -4 、10 -5 、10 -6 isogradient. Draw 100 μL of each gradient solution and evenly spread it on the NA medium plate, repeat each gradient 3 times, place in a constant temperature incubator at 28°C for 1-2 days, pick different single colonies and purify by streaking on the plate. A number of purified strains were screened with Tomato neck rot and roo...

Embodiment 2

[0027] Example 2 Antagonistic effect of Bacillus veleisi on target pathogenic bacteria

[0028] Tomato neck rot root rot fungus (Fusarium oxysporum tomato neck rot root rot specialized type, Fusarium oxysporum f.sp. radicis-lycopersici ), tomato solanacearum (Raueria solanacearum, Ralstonia solanacearum ), Fusarium wilt of tomato (Fusarium oxysporum tomato-specific type, Fusarium oxysporum f. sp. Lycopersici Snyder et al. Hans ), tomato brown root rot (Acanthosporium tomato, Pyrenochaeta lycopersici Schneider et Gerlach), Strawberry wilt ( Fusarium oxysporum f.sp. fragriae ), corn stalk rot (Fusarium graminearum, F. graminearum ) to activate the spare.

[0029] On the ultra-clean workbench, pre-activated vigorous growth of tomato neck rot root rot, tomato brown root rot, tomato Fusarium wilt, strawberry Fusarium wilt, and corn stem base with a diameter of 7 mm were respectively inoculated in the center of the PDA solid medium plate. Bacillus rot bacteria ...

Embodiment 3

[0035] Example 3 Pot control effect on tomato neck rot and root rot

[0036] Pick the single colony of Bacillus Velez CGMCC No.19215 described in Example 1 that has been activated, inoculate it in a 500 mL Erlenmeyer flask containing about 300 mL of sterilized LB liquid medium, and shake it at 37°C and 150 rpm Bed cultured for 24 h, the concentration of bacteria obtained was 10 7 ~10 9 cfu / mL of Bacillus veleisi suspension.

[0037]Soil was taken from the plots where tomato neck rot and root rot occurred seriously, and placed in plastic flower pots with a diameter of 15 cm, and the cultured tomato neck and root rot bacteria were artificially inoculated. Transplant tomato seedlings, 1 plant / pot, and treat 30 mL of tomato roots in each pot of the treatment group with a concentration of 10 7 ~10 9 cfu / mL of Bacillus Velez CGMCC No.19215 bacteria suspension, the control group replaced the bacteria solution with water. Each treatment was replicated 4 times, and each replicat...

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Abstract

The invention provides Bacillus velezensis with the preservation number of CGMCC No.19215. The Bacillus velezensis and fermentation liquor and metabolites thereof can be used for inhibiting phytopathogen and promoting plant growth. The Bacillus velezensis has the biocontrol and growth-promoting effects, can effectively reduce soil-borne diseases of crops such as tomatoes, strawberries and corn, and reduces the usage amount of chemical agents. A preparation prepared from the Bacillus velezensis is stable in storage performance and good in control effect, can be in direct contact with crop rootsystems, has no adverse effect on plant growth and development, and is convenient to process and low in production and transportation cost.

Description

technical field [0001] The invention belongs to the field of biopesticides, and in particular relates to a functional microorganism, in particular to a strain of Bacillus Velez antagonizing various pathogenic fungi and bacteria. Background technique [0002] Bacillus Velez ( Bacillus velezensis ) is a new species of Bacillus, which can produce a variety of substances with antibacterial effects, such as lipopeptides, antibacterial proteins, cyclic dipeptides, etc., which can promote plant growth and resist pathogenic microorganisms in agricultural production. With broad-spectrum antibacterial activity, it is a microorganism with great potential for the development of biopharmaceuticals. [0003] Tomato soil-borne diseases are becoming more and more serious, and have become an important factor restricting tomato production. In recent years, tomato neck rot, tomato brown root rot, and tomato bacterial wilt are the most common damages on facility tomatoes. Tomato neck rot and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 王红艳仇月姜莉莉王开运闫浩浩
Owner SHANDONG AGRICULTURAL UNIVERSITY
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