A Strain of Bacillus Veles and Its Application
A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of poor control efficiency, limited range of control, insufficient colonization ability of antagonistic bacteria, etc., and achieve production and transportation Low cost, good control effect and convenient processing
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Embodiment 1
[0021] Example 1 Isolation, Purification and Identification of Bacillus Velez
[0022] In October 2017, in Fangcun Town, Daiyue District, Tai'an City, Shandong Province, the soil of greenhouses with better facilities to solve the obstacles of continuous sitting was collected, and bacterial strains were isolated from it. In a 250 mL sterilized Erlenmeyer flask filled with 90 mL of sterile water and a small amount of glass beads, place the Erlenmeyer flask on a shaker at 37 °C and 180 rpm for 30 min to fully suspend it, then treat it in a water bath at 80 °C for 10 min, and use Bacterial water to dilute the bacterial suspension to 10 -4 、10 -5 、10 -6 isogradient. Draw 100 μL of each gradient solution and evenly spread it on the NA medium plate, repeat each gradient 3 times, place in a constant temperature incubator at 28°C for 1-2 days, pick different single colonies and purify by streaking on the plate. A number of purified strains were screened with Tomato neck rot and roo...
Embodiment 2
[0027] Example 2 Antagonistic effect of Bacillus veleisi on target pathogenic bacteria
[0028] Tomato neck rot root rot fungus (Fusarium oxysporum tomato neck rot root rot specialized type, Fusarium oxysporum f. sp. radicis-lycopersici ), tomato solanacearum (Raueria solanacearum, Ralstonia solanacearum ), Fusarium wilt of tomato (Fusarium oxysporum tomato-specific type, Fusarium oxysporum f. sp. Lycopersici Snyder et Hans ), tomato brown root rot (Acanthosporium tomato, Pyrenochaeta Lycopersici Schneider et Ger lach), Strawberry wilt ( Fusarium oxysporum f.sp. fragriae ), corn stalk rot (Fusarium graminearum, F. graminearum ) to activate the spare.
[0029] On the ultra-clean workbench, pre-activated vigorous growth of tomato neck rot root rot, tomato brown root rot, tomato Fusarium wilt, strawberry Fusarium wilt, and corn stem base with a diameter of 7 mm were respectively inoculated in the center of the PDA solid medium plate. Bacillus rot bacteria c...
Embodiment 3
[0035] Example 3 Pot control effect on tomato neck rot and root rot
[0036] Pick the single colony of Bacillus Velez CGMCC No.19215 described in Example 1 that has been activated, inoculate it in a 500 mL Erlenmeyer flask containing about 300 mL of sterilized LB liquid medium, and shake it at 37°C and 150 rpm Bed cultured for 24 h, the concentration of bacteria obtained was 10 7 ~10 9 cfu / mL of Bacillus veleisi suspension.
[0037]Soil was taken from the plots where tomato neck rot and root rot occurred seriously, and placed in plastic flower pots with a diameter of 15 cm, and the cultured tomato neck and root rot bacteria were artificially inoculated. Transplant tomato seedlings, 1 plant / pot, and treat 30 mL of tomato roots in each pot of the treatment group with a concentration of 10 7 ~10 9 cfu / mL of Bacillus Velez CGMCC No.19215 bacteria suspension, the control group replaced the bacteria solution with water. Each treatment was replicated 4 times, and each replicat...
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