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Collagen-lysozyme antibacterial film and preparation method thereof

A technology of collagen and lysozyme, applied in biochemical equipment and methods, flexible coverings, packaging, etc., can solve problems such as complicated preparation methods, waste of time and energy, and limited storage period length, and achieve biophase Good capacity, saving time and energy, and extending the shelf life

Active Publication Date: 2017-09-19
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, collagen does not have antibacterial activity, and it does not have the effect of inhibiting or killing the microorganisms polluted on the food surface during processing, storage and handling, so it has limitations in the length of food shelf life
[0003] In addition, in the prior art, in the process of preparing the packaging film, the finished packaging film material is generally prepared first, and then the finished material is measured by the Oxford cup method to measure the antibacterial zone against Escherichia coli and Staphylococcus aureus to test the antibacterial properties of the finished material. Effect, if the antibacterial effect obtained by the test is not good, it is necessary to adjust the concentration of the antibacterial agent to prepare the packaging film again, and then test the antibacterial effect until the finished material of the packaging film with good antibacterial effect is obtained. This preparation method lacks scientific guidance. The preparation method Complicated and time-consuming, wasting a lot of time and energy

Method used

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  • Collagen-lysozyme antibacterial film and preparation method thereof
  • Collagen-lysozyme antibacterial film and preparation method thereof
  • Collagen-lysozyme antibacterial film and preparation method thereof

Examples

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preparation example Construction

[0032] The first aspect of the present invention provides a kind of preparation method of collagen-lysozyme antibacterial membrane, comprises the following steps:

[0033] Take the modified collagen solution, dissolve lysozyme of different quality in the modified collagen solution respectively, after stirring evenly, obtain collagen-lysozyme blends with different lysozyme concentrations;

[0034] The minimum inhibitory concentration test of collagen-lysozyme blend was carried out to obtain the minimum inhibitory concentration of lysozyme;

[0035] The minimum bactericidal concentration test is carried out with the collagen-lysozyme blend having a minimum inhibitory concentration and a concentration greater than the minimum inhibitory concentration, to obtain the minimum bactericidal concentration of lysozyme;

[0036] Collagen-lysozyme blend liquid with the minimum bactericidal concentration and concentration close to the minimum bactericidal concentration is vacuum degassed, ...

Embodiment 1

[0078] refer to figure 1 A schematic flow chart of the preparation method, a preparation method of collagen-lysozyme antibacterial film, comprising the following steps:

[0079] (1) Thermal modification of collagen: Weigh 5g of fish scale collagen and dissolve it in 95mL of distilled water, the thermal modification temperature is 20°C, and the thermal modification time is 30min to obtain a modified collagen solution;

[0080] (2) The minimum inhibitory concentration test of lysozyme: take a certain amount of lysozyme, dissolve it in the modified collagen solution respectively, and the stirring speed is 2000r / min, and the final concentrations of lysozyme are respectively 100, 50, 25, 12.5 , 6.25, 3.13, 1.56, 0.78, 0.39, 0.195 and 0.098mg / mL collagen-lysozyme blend;

[0081] Take a sterile 96-well cell culture plate, place collagen-lysozyme blends with lysozyme concentrations of 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78, 0.39, 0.195 and 0.098 mg / mL respectively in Tubes 1 to 1...

Embodiment 2

[0087] A preparation method of collagen-lysozyme antibacterial film, comprising the following steps:

[0088] (1) Thermal modification of collagen: Weigh 3g of fish scale collagen and dissolve it in 97mL of distilled water, the thermal modification temperature is 60°C, and the thermal modification time is 5min to obtain a modified collagen solution;

[0089] (2) with the step (2) of embodiment 1;

[0090] (3) with the step (3) of embodiment 1;

[0091] (4) Vacuum degassing: Cool the collagen-lysozyme blend solution with a lysozyme concentration of 25mg / mL to room temperature (23°C), degas for 3min in a vacuum of 0.09Mpa, repeat three times, and then let it stand for 1h;

[0092] (5) Casting film formation: the collagen-lysozyme blend solution was fixed to 100mL, that is, 100mL vacuum degassed collagen-lysozyme blend solution with a lysozyme concentration of 25mg / mL was cast onto a plexiglass plate , Dry at 60°C for 4h, pay attention to keep the plexiglass plate level. After...

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Abstract

The invention provides a preparation method of a collagen-lysozyme antibacterial film. The method comprises the following steps: taking modified collagen solutions, respectively dissolving lysozyme having different qualities into the modified collagen solutions, and uniformly stirring to obtain collagen-lysozyme blending solutions with different concentrations; testing minimal inhibitory concentrations of the collagen-lysozyme blending solutions with the different concentrations to obtain the minimal inhibitory concentration of the lysozyme; testing minimum bactericidal concentrations of the collagen-lysozyme blending solutions which have the minimum inhibitory concentrations and of which the concentrations are more than the minimum inhibitory concentrations to obtain the minimum bactericidal concentrations of the lysozyme; carrying out vacuum degassing on the collagen-lysozyme blending solutions which have the minimum bactericidal concentrations and of which the concentrations are close to the minimum bactericidal concentrations, casting into a film, and removing the film to obtain the collagen-lysozyme antibacterial film. After the minimum inhibitory concentrations and the minimum bactericidal concentrations are obtained by using the preparation method disclosed by the invention, the preparation of the antibacterial film is guided; therefore, the method is simple and feasible.

Description

technical field [0001] The invention relates to the field of food packaging materials, in particular to a collagen-lysozyme antibacterial film and a preparation method thereof. Background technique [0002] Edible packaging film has received widespread attention because it can reduce the loss of flavor substances and the consumption of nutrients in food, prevent microbial infection, and effectively prolong food storage and sales. Among the many edible packaging film carriers, collagen is favored because of its safety, non-toxicity, rich nutrition, good film-forming properties and gas barrier properties. However, collagen does not have antibacterial activity, and does not have the effect of inhibiting or killing the microorganisms polluted on the food surface during processing, storage and handling, so it has limitations in the length of food shelf life. [0003] In addition, in the prior art, in the process of preparing the packaging film, the finished packaging film materi...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L89/00C08K5/00C12Q1/18B65D65/46
CPCB65D65/463B65D65/466C08J5/18C08J2389/00C08K5/0058C08L2201/06C08L2203/16C12Q1/18C08L89/00
Inventor 王喆叶忱王怀雨
Owner SHENZHEN INST OF ADVANCED TECH
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