Method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of plant pathogenic fungus inhibitory activity

A technology of lotus leaf total alkaloids and plant pathogenic fungi, which can be applied in the field of bioengineering and can solve problems such as environmental and human and animal hazards

Inactive Publication Date: 2016-04-27
HUNAN AGRICULTURAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] The object of the present invention is to provide a method and application of the total alkaloids of lotus leaves for the inhibition of phytopathogenic fungi. far-reaching and devastating problems

Method used

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  • Method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of plant pathogenic fungus inhibitory activity
  • Method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of plant pathogenic fungus inhibitory activity
  • Method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of plant pathogenic fungus inhibitory activity

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[0021] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0023] Such as figure 1 As shown, the lotus leaf total alkaloids of the embodiment of the present invention comprises the following steps to the inhibitory activity method of phytopathogenic fungi:

[0024] S101: Preparation of spore suspension: activate the slant of a test tube in which the preserved strains are inserted into potato solid medium, take a slant of a test tube grown for 4 days, pour 50 mL of 0.9% sterile saline into the test tube several times, D...

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Abstract

The invention discloses a method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of the plant pathogenic fungus inhibitory activity. The method includes: preparing spore suspension; preparing the lotus leaf total alkaloids; measuring the inhibitory effect of the lotus leaf total alkaloids; measuring the minimum bactericidal concentration of the lotus leaf total alkaloids; measuring the minimum inhibitory concentration of the lotus leaf total alkaloids. The method has the advantages that the fact that the lotus leaf total alkaloids have evident plant pathogenic fungus inhibitory activity is determined for the first time, the lotus leaf total alkaloids have the highest inhibitory activity on cucumber wilt fusarium, and the minimum bactericidal concentration and minimum inhibitory concentration of the lotus leaf total alkaloids are 160 microgram / mL; due to the fact that the lotus leaf resources in China are rich, extraction of the active alkaloids from the lotus leaves is low in cost, nontoxic and harmless; the lotus leaf total alkaloids are used for preventing and treating plant diseases, can be used as potential biological pesticide, and is promising in development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for inhibiting activity of lotus leaf total alkaloids on plant pathogenic fungi and its application. Background technique [0002] The lotus leaf is the leaf of the perennial aquatic herbaceous plant lotus (Nelumbonucifera Gaertn) of the family Nymphaeaceae, also known as lotus leaf and lotus leaf. my country is rich in lotus leaf resources, the most widely distributed in the three river basins of the Yangtze River, the Yellow River and the Pearl River. According to traditional medicine, the lotus leaf tastes bitter and flat, and belongs to the liver, spleen, and stomach meridian. According to the "Compendium of Materia Medica" it is recorded that "taking lotus leaves makes people thin and weak", "grows vitality, helps the spleen and stomach, astringent essence and turbidity, dissipates congestion, reduces swelling and pain, and causes acne". In Marc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18A01N65/08A01P3/00
CPCA01N65/08C12Q1/18
Inventor 肖桂青卢向阳魏琦超
Owner HUNAN AGRICULTURAL UNIV
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