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Application of L-arginine in improvement of antibiotics sensitivity of bacteria

A technology of antibiotics and arginine, applied in the field of medicine, achieves good application prospects, overcomes bacterial resistance, high safety and operability

Active Publication Date: 2018-05-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on whether L-arginine can improve the ability of antibiotics to clear pathogenic bacteria.

Method used

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  • Application of L-arginine in improvement of antibiotics sensitivity of bacteria
  • Application of L-arginine in improvement of antibiotics sensitivity of bacteria
  • Application of L-arginine in improvement of antibiotics sensitivity of bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 L-arginine improves the sensitivity of Edwardsiella tarda to kanamycin

[0038] 1. Determination of drug resistance of Edwardsiella tarda EIB202

[0039] Edwardsiella tarda is a Gram-negative short bacillus, which was first reported by Hoshina in 1962 and is related to reddisease of Japanese eels. From the first report to the present, the fungus has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, carp, etc., causing huge losses to aquaculture. Edwardsiella tarda is also a common pathogenic bacteria in humans and fish, which directly poses a threat to human health.

[0040] Firstly, the minimum inhibitory concentration of Edwardsiella tarda to various antibiotics was determined. The results showed that the minimum inhibitory concentration of Edwardsiella tarda EIB202 to kanamycin was 12.5 μg / mL, the minimum inhibitory concentration to tetracycline was 125 μg / mL, and the minimum inhibitory concentrati...

Embodiment 2

[0055] Example 2 L-arginine improves the sensitivity of Edwardsiella tarda to other antibiotics

[0056] In order to know whether the addition of L-arginine can improve the sensitivity of Edwardsiella lentus to antibiotics other than kanamycin, the bacterial samples were prepared according to the method in step 2 of Example 1. The experiment was divided into 3 groups: 2 control groups (control group 1: no addition of antibiotics and L-arginine; control group 2: addition of antibiotics only) and 1 experimental group (addition of an antibiotic and L-arginine) . The added antibiotics and their concentration were: 12.5 μg / mL ampicillin, 2 μg / mL baroxacin and 50 μg / mL cefazolin sodium. After 6 hours of action, the number of viable bacteria was counted to calculate the survival rate.

[0057] The result is as Figure 5 As shown, after adding L-arginine, the sensitivity of Edwardsiella tarda to ampicillin was increased by 5.13 times (survival rate dropped from 60.03% of only addin...

Embodiment 3

[0059] Example 3 L-arginine improves the sensitivity of various bacteria to kanamycin

[0060] 1. Preparation of bacteria solution

[0061] Pick Escherichia coli K12, Pseudomonas aeruginosa, Streptococcus B, and Staphylococcus aureus (MASA) and other bacterial monoclonals into 100mL LB liquid medium, at 37°C or 30°C, 200rpm Under culture for 16h to reach saturation. Collect 20 mL of each bacterial liquid, centrifuge at 8000 rpm for 5 min, remove the supernatant and wash the cells with an equal volume of 0.85% normal saline, and finally suspend the cells with 1× M9 basic liquid medium (containing 10 mM acetate) , adjust the OD value of the bacterial solution to 0.5, and then aliquot 5 mL into test tubes for later use.

[0062] 2. L-arginine increases the sensitivity of various bacteria to kanamycin

[0063] The above-mentioned prepared bacterial solution was divided into 3 groups according to the type of bacteria: 2 control groups (control group 1: no addition of kanamycin a...

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Abstract

The invention belongs to the technical field of medicine and particularly discloses application of L-arginine in improvement of antibiotics sensitivity of bacteria. According to the application, through improving a proton motive force of the bacteria by the L-arginine, the quantity of antibiotics entering interior of the bacteria can be increased, and finally, the bacteria die, so that the L-arginine can be used for improving the sensitivity of the bacteria to the antibiotics, and thus, the problem of bacterial drug resistance is solved. Through jointly using the L-arginine and the antibiotics, the bactericidal action of the antibiotics can be remarkably improved, and the effect is better and the safety and operability are higher compared with that in the prior art that only the antibiotics serve as an antibacterial drug.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of L-arginine in improving the susceptibility of bacteria to antibiotics. Background technique [0002] Pathogenic bacteria seriously endanger human health and the sustainable development of the breeding industry, and effective measures need to be taken to prevent and control them. Since Fleming (Fleming) discovered penicillin in 1929, a large number of studies on various antibiotics have opened the era of human antibiotics, which play an important role in the treatment of infectious diseases, making the use of antibiotics increasingly widespread. Although the use of antibiotics can effectively prevent and control diseases, the overuse and misuse of antibiotics can lead to bacterial resistance. Resistant strains develop resistance to otherwise effective antibiotics, making infections difficult to control. Therefore, it is very important to adopt new...

Claims

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Application Information

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IPC IPC(8): A61K31/198A61K31/7036A61K31/43A61K31/4709A61K31/546A61P31/04
CPCA61K31/198A61K31/43A61K31/4709A61K31/546A61K31/7036A61K2300/00Y02A50/30
Inventor 李惠彭博彭宣宪叶锦州
Owner SUN YAT SEN UNIV
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