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Method for establishing lily gene transforming receptor system

A technology of gene transformation and lily, which is applied in the field of establishment of lily gene transformation receptor system, can solve the problem that the high-frequency regeneration system of lily cannot be well established, achieve strong flexibility and wide applicability, and the method is simple and easy Effect

Inactive Publication Date: 2005-01-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on lily tissue culture, but the high-frequency regeneration system of lily based on gene transformation has not been well established.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The experimental variety is Oriental lily, and its bulbs are used as explants.

[0015] Peel the bulb from the outside to the inside, rinse it with clean water, soak it in washing powder for 30 minutes, and then sterilize it on the aseptic operating table: soak it in 75% alcohol for 2 minutes, rinse it three times with sterile water, and use 20% concentration of commercially available Antifoam Soak for 15-20min, rinse with sterile water for 3 times, cut it into 5mm size and use it as explant for later use.

[0016] Bulbs were inoculated on basic media such as MS, WS, B5 and Nitsch medium, and the growth was observed after 1 month. Determine the basal medium as enriched element balanced medium MS.

[0017] Inoculate the bulbs on MS+BA 0.5-2.0mg / l+NAA 0.5-2.0mg / l+2,4-D 0.1-1.0mg / l (pH5.8) solid differentiation medium and subculture once every 2 weeks After 45 days, the differentiation rate was counted. According to the results, the optimal differentiation medium was de...

Embodiment 2

[0022] The experimental variety was Lily musk, and its filaments were used as explants.

[0023] Cut off the whole flower bud of the lily musk, rinse it with clean water, soak it in washing powder for 30 minutes, and put it on a sterile operating table for disinfection. The method is as follows: put the material into a sterile container, disinfect it with 75% alcohol for 30 seconds, then treat it with 20% commercially available sodium hypochlorite solution for 10 minutes, rinse it with sterile water for 6-8 times, and finally dry it with sterile absorbent paper water, peel off the flower buds, separate the filaments and cut them into 5-8mm segments as explants for subsequent use.

[0024] The filaments were inoculated on basic media such as MS, WS, B5 and Nitsch medium, and the growth was observed after 1 month. Determine the basal medium as enriched element balanced medium MS.

[0025] Inoculate the explants on MS+BA (or KT) 0.25-2.0mg / l+NAA (or IAA or 2,4-D) 0.25-2.0mg / l (...

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PUM

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Abstract

A method for creating the gene transfer receptor system of lily includes disinfecting the surface of lily bulb or filament, cutting the become segments, culturing in differentiating culture medium to create a high-frequency regeneration system, antibiotic sensitivity test to determine the screening concentrations of cephamycin, hygromycin and kanamycin, inducing the rooting adventitious embryo, and transplanting.

Description

technical field [0001] The invention relates to a method for establishing a lily gene transformation receptor system, which is used in the field of plant gene transformation receptor biotechnology. Background technique [0002] Lily is a world-famous flower with a wide range of applications. The improvement of its quality has been dependent on traditional genetic breeding methods for a long time. The rapid development of plant molecular biology has provided a new way to improve plant quality and traits. It has become feasible to use transgenic means to improve lily traits and quality, and a good receptor system is a key technical link in lily gene transformation. The simplicity of the transformation method and the frequency of transformation and regeneration directly determine the establishment of a plant receptor system with high efficiency, stability and strong regeneration ability is a prerequisite for the realization of gene transformation. There have been many reports...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04C12N15/82
Inventor 唐东芹杨学军钱虹妹黄丹枫
Owner SHANGHAI JIAO TONG UNIV
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