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Method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli

A technology of Riemer's and Escherichia coli, applied in the biological field, can solve the problems of differentiation, high mortality rate of duck flocks, blocking the process of co-growth, etc.

Active Publication Date: 2015-12-23
山东绿州动物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Clinically, Riemerella anatipestifer and Escherichia coli are often co-infected. Both bacteria can infect ducks of different breeds, ages and sexes. Characteristic pathological changes, clinical diagnosis is difficult to distinguish between the two, and the mortality rate of ducks infected with the two diseases is high, and the elimination rate is high, causing great economic losses to the duck industry
[0004] Clinically, it is often found that there is a transfer of drug resistance between the two bacteria, but in the process of in vitro culture, Escherichia coli uses its competitive advantage to force the production of Riemerella anatipestifer to stop production in a short period of time, thereby blocking the two. co-growth process, making in-depth study impossible

Method used

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  • Method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli
  • Method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli

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Embodiment 1

[0041] The method for the mixed cultivation and re-isolation of Riemerella anatipestifer and Escherichia coli adopts the following steps:

[0042] (1) Bacterial recovery:

[0043] (1-1) Recovery of Riemerella anatipestifer:

[0044] Measure 1ml of normal saline, dissolve and mix an appropriate amount of freeze-dried and preserved R. anatipestifer strains in the normal saline to prepare a suspension of R. anatipestifer. solution was inoculated on tryptone soy agar medium containing 4% serum, and placed in CO 2Cultivate in an incubator at 37°C for 20-24h, then pick a single colony from the medium and inoculate it in a tryptone soybean broth medium containing 4% serum, on a shaker with a rotation speed of 150r / min and a temperature of 37°C Vibrating culture for 20-24h to obtain recovered Riemerella anatipestifer;

[0045] The Riemerella anatipestifer strain is provided by the Poultry Research Institute of Shandong Academy of Agricultural Sciences.

[0046] (1-2) Escherichia c...

Embodiment 2

[0067] The method for the mixed cultivation and re-isolation of Riemerella anatipestifer and Escherichia coli adopts the following steps:

[0068] (1) Bacterial recovery:

[0069] ① Recovery of Riemerella anatipestifer:

[0070] Measure 1ml of normal saline, dissolve and mix an appropriate amount of freeze-dried and preserved R. anatipestifer strains in the normal saline to prepare a suspension of R. anatipestifer. solution was inoculated on tryptone soy agar medium containing 4% serum, and placed in CO 2 Cultivate in an incubator at 37°C for 20-24h, then pick a single colony from the medium and inoculate it in a tryptone soybean broth medium containing 4% serum, on a shaker with a rotation speed of 150r / min and a temperature of 37°C Vibrating culture for 20-24h to obtain recovered Riemerella anatipestifer;

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Abstract

The invention discloses a method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli comprises the following steps of (1) performing strain recovery; (2) screening riemerella anatipestifer and escherichia coli with antibiotics sensitivity differences; (3) inoculating the riemerella anatipestifer and the escherichia coli into a culture medium for mixed culture at a certain time interval or different diluting concentration; (4) measuring the minimum inhibitory concentration of antibiotics in the second step on the screened riemerella anatipestifer and escherichia coli; (5) preparing an antibiotic plate culture medium; (6) separating mixed culture bacterium liquid. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli has the advantages that two kinds of bacteria are used outside duck poultry bodies and can jointly grow for more than 20 generations; conditions are provided for studying drug resistance transfer and relevant mechanisms of the two kinds of bacteria under the symbiosis condition; important significance is realized on disease prevention and control of duck breeding industry.

Description

(1) Technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for mixed culture and re-isolation of Riemerella anatipestifer and Escherichia coli. (2) Background technology [0002] Riemerella anatipestifer is the pathogenic bacterium that causes Riemerella anatipestifer. Riemerella anatipestifer causes acute or chronic septicemia in ducklings and is a major disease that seriously endangers the duck industry all over the world. Escherichia coli is the causative agent of the most economically costly bacterial disease in poultry farming. [0003] Clinically, Riemerella anatipestifer and Escherichia coli are often co-infected. Both bacteria can infect ducks of different breeds, ages and sexes. Characteristic pathological changes, clinical diagnosis is difficult to distinguish between the two, and the mortality rate of ducks infected with the two diseases is high, and the elimination rate is high, causing great economic losses to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/19C12R1/01
Inventor 林树乾冯敏燕傅剑李翠郝会元姜新社丁衍涛杨世发宋敏训黄中利赵增成李桂明
Owner 山东绿州动物药业有限公司
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