Method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli
A technology of Riemer's and Escherichia coli, applied in the biological field, can solve the problems of differentiation, high mortality rate of duck flocks, blocking the process of co-growth, etc.
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Embodiment 1
[0041] The method for the mixed cultivation and re-isolation of Riemerella anatipestifer and Escherichia coli adopts the following steps:
[0042] (1) Bacterial recovery:
[0043] (1-1) Recovery of Riemerella anatipestifer:
[0044] Measure 1ml of normal saline, dissolve and mix an appropriate amount of freeze-dried and preserved R. anatipestifer strains in the normal saline to prepare a suspension of R. anatipestifer. solution was inoculated on tryptone soy agar medium containing 4% serum, and placed in CO 2Cultivate in an incubator at 37°C for 20-24h, then pick a single colony from the medium and inoculate it in a tryptone soybean broth medium containing 4% serum, on a shaker with a rotation speed of 150r / min and a temperature of 37°C Vibrating culture for 20-24h to obtain recovered Riemerella anatipestifer;
[0045] The Riemerella anatipestifer strain is provided by the Poultry Research Institute of Shandong Academy of Agricultural Sciences.
[0046] (1-2) Escherichia c...
Embodiment 2
[0067] The method for the mixed cultivation and re-isolation of Riemerella anatipestifer and Escherichia coli adopts the following steps:
[0068] (1) Bacterial recovery:
[0069] ① Recovery of Riemerella anatipestifer:
[0070] Measure 1ml of normal saline, dissolve and mix an appropriate amount of freeze-dried and preserved R. anatipestifer strains in the normal saline to prepare a suspension of R. anatipestifer. solution was inoculated on tryptone soy agar medium containing 4% serum, and placed in CO 2 Cultivate in an incubator at 37°C for 20-24h, then pick a single colony from the medium and inoculate it in a tryptone soybean broth medium containing 4% serum, on a shaker with a rotation speed of 150r / min and a temperature of 37°C Vibrating culture for 20-24h to obtain recovered Riemerella anatipestifer;
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