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Preparing method for cow mammitis E.coli culture fluid and its use

A technology for dairy cow mastitis and Escherichia coli, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of expensive acceptance, inability to truly reflect antibiotic sensitivity, etc., and achieve the effect of cheap equipment

Inactive Publication Date: 2006-11-08
兆丰华生物科技(南京)有限公司
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AI Technical Summary

Problems solved by technology

These methods not only need at least 3-5 days to get the results, but also have the necessary equipment, reagents and technical conditions for bacteriological identification, and the results of drug susceptibility tests with single colonies sometimes cannot truly reflect the sensitivity of the bacterial "population" to antibiotics sex
As for the automated drug sensitivity instrument and Etest method, it is difficult to be accepted by domestic veterinary clinics and dairy farms due to their high price

Method used

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  • Preparing method for cow mammitis E.coli culture fluid and its use
  • Preparing method for cow mammitis E.coli culture fluid and its use
  • Preparing method for cow mammitis E.coli culture fluid and its use

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Embodiment Construction

[0017] 1. Preparation of MQQ culture medium:

[0018] Weigh 10g of peptone, 1.5g of No. 3 bile salt, 10g of lactose, 5g of sodium chloride, and 5g of sodium citrate with a balance, add the above ingredients into 1000ml of water, and stir until completely dissolved. Then adjust the pH value to 7.2 with 10mol sodium hydroxide solution. Then add 0.5ml of 1% crystal violet aqueous solution and 4ml of 0.4% phenol red aqueous solution to the solution, stir and mix thoroughly, then sterilize the container at 121°C for 15 minutes, and store at 4°C for later use.

[0019] It is also possible to add 15 grams of agar powder per liter of liquid culture solution to form an MQQ agar plate.

[0020] 2. Rapid identification of Escherichia coli:

[0021] According to the clinical examination or the results of the California Mastitis Test (CMT), it is preliminarily judged that the cows have clinical or latent mastitis. The first three handfuls of milk are squeezed out by conventional milking ...

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Abstract

The preparation process of cow mammitis E. coli culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, No. 3 bile salt 1.5 g, lactose 10 g, sodium chloride 5 g and sodium citrate 5 g in water of 1000ml; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 1% concentration crystal violet water solution in 0.5 ml and 0.4% concentration phenol red water solution in 4 ml; sterilizing at 121 deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis E. coli and antibiotic sensitivity test.

Description

technical field [0001] The invention belongs to the technical field of animal epidemic diagnosis and prevention. technical background [0002] Dairy cow mastitis has a high incidence and great harm, and is one of the most important diseases affecting the development of the dairy industry in the world. According to the International Milk Federation, about 50% of cows suffer from mastitis, of which about 2% suffer from clinical mastitis and 48% suffer from recessive mastitis. The incidence rate of clinical mastitis in my country is 33.41% (9.7%-55.6%), and the detection rate of recessive mastitis is 50-80%. Clinical mastitis has obvious clinical symptoms such as decreased milk production, breast swelling, and milk deterioration, while subclinical mastitis has no visible changes in the appearance of breasts and milk, but often causes the quality of raw milk to decline, threatening people's health. healthy. It is estimated that the annual economic loss caused by mastitis in m...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/10
Inventor 孙怀昌钱科仇华磊张鑫宇
Owner 兆丰华生物科技(南京)有限公司
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