Biological chips sample application platform

Abstract

The invention discloses a biochip spotting platform which relates to the preparation of a microarray biochip. The biochip spotting platform mainly utilizes a precision linear motor which can carry out the high-speed motion for constituting a three-dimensional distribution mechanical hand, and simultaneously comprises a spotting head with 1 to 48 spotting needles, a chip fixed platform, a sample box platform and a spotting needle cleaning and drying device are arranged on a working platform with the water bath cooling function, an electric control box and the distribution mechanical hand are operated under the control of software of a computer, thereby rapidly completing the spotting and finally forming the complete biochip. The spotting platform is characterized by compact structure, simple and convenient operation, high speed, efficiency and precision, less cross-infection and broad applicability, etc., thereby being very applicable to the scale production of the biochips and also being applicable to scientific research and applications.

Description

technical field [0001] The invention relates to the preparation of a microarray biochip, more precisely, it relates to a rapid spotting platform of a microarray biochip for multi-indicator and multi-person parallel detection. Background technique [0002] Several existing biochip preparation methods can be basically divided into two categories: one is in-situ synthesis (i.e. directly synthesized oligonucleotide probes on the surface of the support in situ); the other is pre-synthesized Direct spotting, the trace DNA solution is directly placed in the form of a microarray and solidified on the chip carrier. Because the in situ synthesis method is very complicated, most of the DNA microarray chips use the synthetic spotting method. The preparation of the protein chip, due to the limitation of the method, is prepared by the method of first synthesis and then spotting. The synthetic spotting method is to accurately and quickly spot different probe samples quantitatively on pos...

AI Technical Summary

Problems solved by technology

[0003] The existing biochip spotting instruments are mainly divided into two types: one is a small spotting instrument for individuals, laboratories and hospitals, which is flexible in operation and can meet various individualized spotting requirements, and the price is low. But the sampling efficiency is low, the accuracy and work stability are poor, suitable for scientific research and other simple applications

The other is a large workstation provided to biochip manufacturers or professional research institutions. These devices mostly use sophisticated electronic control platforms to form manipulators, which have significantly improved motion accuracy, spotting efficiency, and work stability, but their prices are high. , so this type of equipment is suitable for in-depth research and production by biochip manufacturers or professional research institutions with certain strength, and most of this type of equipment is imported from abroad. Due to its high price and high cost of consumables, it is not suitable for domestic promotion.

In addition, most of the current biochip spotting equipment is designed to spot the probes on rigid carriers such as glass slides, and rarely on soft carriers such as membranes, and the substrate for spotting samples is relatively single.

[0004] However, with the development of biochip technology, the demand for biochips has increased, and the requirements for biochip spotters have also been further improved, developing in the direction of higher precision, faster speed, higher efficiency, and wider applicability. Existing biochip spotters can no longer meet the needs of this rapid and large-scale production

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