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Excrement metabolite for detecting curative effect of active pulmonary tuberculosis and detection system thereof

A technology of active pulmonary tuberculosis and detection system, applied in the field of biomarkers, can solve the problems of low sensitivity and insufficient resolution, and achieve the effects of good repeatability, small injection volume and no sample

Inactive Publication Date: 2020-12-18
广东省结核病控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest disadvantage of NMR technology is low sensitivity, insufficient resolution, and high-abundance analytes often mask low-abundance analytes

Method used

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  • Excrement metabolite for detecting curative effect of active pulmonary tuberculosis and detection system thereof
  • Excrement metabolite for detecting curative effect of active pulmonary tuberculosis and detection system thereof
  • Excrement metabolite for detecting curative effect of active pulmonary tuberculosis and detection system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A detection system for detecting the curative effect of active pulmonary tuberculosis, comprising parameter acquisition equipment and a data processing device with the following data processing functions:

[0041] The results of the test patient's stool detected by LC-MS will be analyzed, and the conclusion will be output according to the following standard: if the relative expression of one or more of the 14 differential metabolites in the test patient's stool is less than the average value of the T6 group and If the relative expression levels are all ≥ the average value of the LTBI group, it indicates that the patient to be tested has been cured or is a candidate for cure; the above 14 differential metabolites include 11,14-trans-Eicosadienoic acid 11,14-trans-eicosadienoic acid , Kynurenic acid, Ethylrhoeagenine, Sulfanilamide, L-Glutamic acid L-glutamic acid, 4-Acetamidoantipyrine, L-Alanine L-alanine, Oxindole 2 - Indolinone, 2-Mercaptobenzothiazole 2-Mercaptobenzo...

Embodiment 2

[0048] Example 2 A method for judging active pulmonary tuberculosis based on feces LC-MS technology

[0049] 1. Inclusion and grouping of research objects

[0050] The included population is from Shenzhen Chronic Hospital, aged 18-60 years, with regular work, rest and diet, and no alcoholism or smoking. The specific groups are as follows:

[0051] (1) Healthy group infected with tuberculosis (LTBI) (29 people)

[0052] People who are positive in PPD skin test and positive in interferon-γ release test, but without any symptoms and signs of tuberculosis and related mycobacterial diseases, and can not be diagnosed with pulmonary tuberculosis, including those who have close contact with patients who excrete bacteria, and those who have been in close contact with tuberculosis prevention and control clinicians. High-risk groups such as laboratory physicians.

[0053] (2) The group of patients who were newly diagnosed with tuberculosis and did not receive treatment (37 people)

...

Embodiment 3

[0097] Embodiment 3 evaluates the validation of the curative effect of active pulmonary tuberculosis in patients to be tested

[0098] The feces of 3 active tuberculosis patients (ATB), 3 latent Mtb healthy people, 2 tuberculosis patients treated for 2 months (T2) and 2 tuberculosis patients cured after 6 months of treatment (T6) were routinely collected ( Feces come from Shenzhen Chronic Disease Hospital, all patients are determined by clinical diagnosis, and patients and volunteers are informed), according to the method of judging active pulmonary tuberculosis patients based on feces LC-MS metabolomics analysis technology in Example 1, respectively get Relative expression of 14 differential metabolites. 14 differential metabolites including 11,14-trans-Eicosadienoic acid 11,14-trans-eicosadienoic acid, Kynurenic acid, Ethylrhoeagenine, Sulfanilamide, L-Glutamic acid L-glutamic acid Amino Acid, 4-Acetamidoantipyrine 4-Acetamidoantipyrine, L-Alanine L-Alanine, Oxindole 2-Indo...

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Abstract

The invention discloses a method for judging whether an active pulmonary tuberculosis patient to be detected is cured or not after being treated for 6 months by an antituberculosis drug standard basedon an excrement LCMS metabonomics technology. The method comprises the following steps: carrying out LCMS (Liquid Chromatography Mass Spectrometry) detection on excrement of a to-be-detected patientto respectively obtain relative expression quantities of 14 differential metabolites, and judging whether the to-be-detected patient is cured or not according to the relative expression quantities ofthe 14 differential metabolites. Compared with the traditional method, the method provided by the invention has the advantages of non-invasion, simple sample treatment, no damage to the sample, smallsample injection amount, good repeatability, low cost and the like.

Description

technical field [0001] The present invention relates to the technical field of biomarkers, more specifically, to feces metabolites and a detection system for detecting the curative effect of active pulmonary tuberculosis. Background technique [0002] Tuberculosis (TB) is a widespread and in many cases fatal chronic infectious disease caused by Mycobacterium tuberculosis (Mtb) infection. most. Although the incidence of tuberculosis has been slowly declining in recent years, there were still about 10 million new cases and about 1.7 million deaths worldwide in 2018. Tuberculosis has become a global problem threatening human health, and has become the leading cause of death in some developing countries and regions, especially in areas with a high incidence of AIDS. [0003] Early diagnosis of tuberculosis and timely anti-tuberculosis treatment are of great significance to effectively control the progression of tuberculosis and the spread of Mycobacterium tuberculosis. Curren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/7266
Inventor 魏文静周琳陈亮董文雅张晨晨廖庆华陈瑜晖王嘉雯梁安棋徐华丽
Owner 广东省结核病控制中心
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