Integrated fluorescence scanning system

Abstract

An integrated fluorescence scanning system is provided. The integrated fluorescence scanner combines an embedded computer, light engine, microscope, and motion stage into a compact rack-mountable network appliance that allows for automation of fluorescence microscopy. In an embodiment, the integrated fluorescence scanner includes a solid-state light engine which can provide intense, pure, and stable light across the spectrum required for imaging of all fluorophores of interest. The embedded computer allows for autonomous operation, and network appliance features including synchronous multi-scanner operation and monitoring, multisite operation via a single control terminal, and calibration for inter and intra instrument consistency.

Description

CLAIM OF PRIORITY[0001]This application claims the benefit of priority to U.S. Provisional Application No. 62 / 072,195, filed Oct. 29, 2014 entitled “INTEGRATED FLUORESCENCE SCANNING SYSTEM” which application is incorporated herein in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to a system and method for fluorescence scanning. In particular the present invention relates to an integrated fluorescence scanner featuring a solid state light source.BACKGROUND OF THE INVENTION[0003]Fluorescence microscopy utilizes an optical microscope configured to detect fluorescence and phosphorescence instead of or as well as reflection and absorption of light to study properties of specimens. In fluorescence microscopy, the specimen is illuminated with light of a specific wavelength an excitation filter or dichroic mirror is used to select the wavelength of light directed at the specimen. The excitation light excites fluorophores in the specimen causing the fluorophores to e...

AI Technical Summary

Benefits of technology

The present invention is an integrated fluorescence scanner that merges a light engine, microscope, imaging devices, and motion control into a network appliance that automates fluorescence microscopy. It incorporates a solid-state light engine that provides stable, pure light across the spectrum required for imaging fluorophores. The scanner is self-contained, space-efficient, and scalable for high throughput applications. It also allows for synchronous multi-scanner operation and calibration for inter and intra instrument consistency. Overall, the integrated fluorescence scanner meets the needs of high throughput fluorescence microscopy with a pared down design that employs all the best features of a microscope, the efficiency and power of a solid state light engine, and the fast design cycle and short time to market that most instrument manufacturers need.

Problems solved by technology

The instruments typically offer all the degrees of freedom for analysis that are expected of a microscope, yet are superfluous for a dedicated scanner or reader while adding complexity and unnecessary failure modes and maintenance / setup requirements.

Also, the instruments utilize conventional lighting technologies such as arc lamps which have disadvantages such as spectral instability, short lifespan / high maintenance costs and high heat production.

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