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Detection method and detection system of multi-vitamins in dry blood spot

A detection method and a technology for dried blood spots, which are applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as time-consuming and labor-intensive, and achieve the effects of less trauma, high-throughput analysis, and simple and fast detection methods.

Inactive Publication Date: 2018-06-22
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention provides a detection method and detection system for multivitamins in dried blood spots, which can effectively solve the time-consuming and laborious technical defects in the current detection methods for multivitamins

Method used

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  • Detection method and detection system of multi-vitamins in dry blood spot
  • Detection method and detection system of multi-vitamins in dry blood spot
  • Detection method and detection system of multi-vitamins in dry blood spot

Examples

Experimental program
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Effect test

Embodiment 1

[0083] This embodiment uses the detection method of the present invention to detect fat-soluble vitamins in dried blood spots, and the specific steps are:

[0084] 1. Prepare dried blood spot card samples: drop fresh blood on a special dried blood spot card with a blood volume of 25 μL, and dry it in the dark for 2 hours.

[0085] 2. Use the software to transfer the dried blood spots in step 1 to the pretreatment unit, and spray 5 μL isotope internal standard solution on the dried blood spots in the pretreatment unit; after drying for 5 seconds, the processed dried blood spots are obtained.

[0086] 3. Use the software to transfer the processed dried blood spots to the extraction unit, and extract the target fat-soluble vitamins in the dried blood spots with 20 μL of extraction liquid. After the extraction is finished, the cleaning unit automatically cleans the extraction unit and prepares to extract the next dried blood spot. The specific cleaning process is: S201: the outle...

Embodiment 2

[0102] The present embodiment is fat-soluble vitamin stability test (RSD), and method is as follows:

[0103] In the experiment, the same fresh blood sample was used to make 6 dried blood spots, and the samples were continuously extracted to determine the stability of the vitamin detection method of the dried blood spots. The results are shown in Table 8. The stability of the fat-soluble vitamins in it is good.

[0104] Table 8 Stability data of fat-soluble vitamins

[0105]

Embodiment 3

[0107] This embodiment is to use the detection method of the present invention to detect water-soluble vitamins in dried blood spots, and the specific steps are:

[0108] 1. Prepare dried blood spot card samples: drop fresh blood on a special dried blood spot card with a blood volume of 25 μL, and dry it in the dark for 2 hours.

[0109] 2. Use the software to transfer the dried blood spots in step 1 to the pretreatment unit, and spray 5 μL isotope internal standard solution on the dried blood spots in the pretreatment unit; after drying for 5 seconds, the processed dried blood spots are obtained.

[0110] 3. Use the software to transfer the processed dried blood spots to the extraction unit, and extract the target fat-soluble vitamins in the dried blood spots with 20 μL of extraction liquid. After the extraction is finished, the cleaning unit automatically cleans the extraction unit and prepares to extract the next dried blood spot. The specific cleaning process is: S301: th...

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Abstract

The invention belongs to the technical field of blood detection and particularly relates to a detection method and a detection system of multi-vitamins in a dry blood spot. The invention provides thedetection method of the multi-vitamins in the dry blood spot, which comprises the following steps: S101, adding an internal standard substance in the dry blood spot to obtain a treated dry blood spot;S102, extracting the treated dry blood spot with first extracting liquid to obtain first detection liquid or extracting the treated dry blood spot with second extracting liquid to obtain second detection liquid; and S103, detecting the first detection liquid or the second detection liquid by adopting a liquid chromatography-mass spectrometer, wherein the first extracting liquid is a methanol aqueous solution with a volume percentage of 5-95 percent; and the second extracting liquid is 10-90 percent of methanol aqueous solution. The detection method provided by the invention can detect the multi-vitamins in micro blood within a short time and solves the technical defects of time waste and labor waste in an existing method for detecting the multi-vitamins.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a detection method and a detection system for multivitamins in dried blood spots. Background technique [0002] At present, the commonly used vitamin detection method for the human body is to use plasma detection. The plasma sample is alkalized with sodium hydroxide, precipitated protein and petroleum ether are extracted and concentrated, and then tested on the machine. However, the vitamin detection method in the prior art requires more blood. , the conditions for blood preservation and transportation are harsh, and only a single vitamin content can be detected each time. For the detection of multiple vitamins, the detection methods of the prior art are very time-consuming and laborious. Contents of the invention [0003] In view of this, the present invention provides a detection method and detection system for multivitamins in dried blood spots, which can...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 林芸张旭光贺瑞坤肖健汪玉芳彭辉叶少文苏昭仑
Owner BY HEALTH CO LTD
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