Wheat tissue active oxygen fluorescence labeling method

A fluorescent labeling, reactive oxygen species technology, applied in the field of plant stress and physiological response, can solve the problems of lack of scientific detection method for the comprehensive production of reactive oxygen species, large differences in production, difficult plant stress resistance or aging process, etc. Achieve precise resistance or aging process, easy operation, improve the effect of fixing and loading
CN102589942BInactive Publication Date: 2013-11-13CROP RES INST SHANDONG ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CROP RES INST SHANDONG ACAD OF AGRI SCI
Publication Date
2013-11-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant stress and physiologic response, and particularly relates to the labeling method for active oxygen generation, generation positions and relative content under biotic stress and abiotic stress during the growth and development process of wheat. The labeling method comprises the following steps of: preparing a label load buffer solution, preparing a material tissue block, rinsing the tissue block with the load buffer solution, placing the tissue block in a dark place at 25 DEG C, exciting the tissue block by using a laser excitation device, observing the tissue block and taking a photo. Compared with the traditional labeling method of DAB (Dimethylaminoazobenzene), NBT (Nitroblue Tetrazolium) and other active oxygen species, the labeling method saves time and avoids the distortion of active oxygen content and positions of a sample during long-time labeling load period. When the active oxygen is labeled by DCFH-DA (Dichlorofluorescein Diacetate), the material is fixed, and the accuracy of active oxygen labeling is ensured. The used device has simple requirements and is easy to operate.
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Description

technical field

[0001] The invention belongs to the technical field of plant adversity stress and physiological response, and relates to a fluorescent labeling method for the location and relative content detection of active oxygen in plant tissues, and in particular to a method for detecting active oxygen under biotic and abiotic stress during the growth and development of wheat Marking method of production, production site and relative content. Background technique

[0002] During the growth and development of plants, they are constantly subjected to various adversities and the inevitable aging process, which leads to metabolic disorders of reactive oxygen species in plant cells, that is, reactive oxygen species in plants such as superoxide anions (OH· - ), hydrogen peroxide (H 2 o 2 ), hydroxyl radical (-OH), singlet oxygen ( 1 o 2） and other bursts; and active oxygen scavengers, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), carotenoids, vit...

Claims

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