4,7-dichlorofluorescein dyes as molecular probes

a technology of dimerization and fluorescence, which is applied in the field of fluorescent labelling techniques, can solve the problems of difficult to find three or more dyes with no significant overlap of emission bands, and the requirement is particularly difficult to satisfy, and achieves high fluorescence efficiency
US20060063734A1Inactive Publication Date: 2006-03-23APPL BIOSYSTEMS INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
APPL BIOSYSTEMS INC
Publication Date
2006-03-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5- (and 6-)carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of application Ser. No. 10 / 714,407 filed Nov. 14, 2003, which is a continuation of application Ser. No. 09 / 949,444 filed Sep. 7, 2001, now U.S. Pat. No. 6,649,598, which is a continuation of application Ser. No. 09 / 580,754 filed May 30, 2000, now U.S. Pat. No. 6,403,812, which is a continuation of application Ser. No. 09 / 273,655 filed Mar. 23, 1999, now U.S. Pat. No. 6,096,723 which is a continuation of application Ser. No. which is a continuation of application Ser. No. 08 / 400,780 filed Mar. 8, 1995, now U.S. Pat. No. 5,654,442, which is a continuation of application Ser. No. 07 / 939,813 filed Sep. 3, 1992, Abandoned, which is a continuation-in-part of application Ser. No. 07 / 436,455 filed Nov. 14, 1989, now U.S. Pat. No. 5,188,934, which are all incorporated herein by reference.FIELD OF THE INVENTION

[0002] The invention relates generally to fluorescent labelling techniques, and more particularly, to ...

Claims

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