Separation identification and phosphorus solubilizing capacity measurement method for improved phosphate solubilizing bacteria

A determination method and separation and identification technology are applied in the fields of selective separation and culture of phosphate-solubilizing bacteria and detection of phosphate-solubilizing ability, which can solve the problems of incomplete separation, inconspicuous screening markers, omissions, etc., and achieve the effect of improving the screening ability.

Inactive Publication Date: 2014-03-26
YUNNAN REASCEND TOBACCO TECH GRP +1
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AI Technical Summary

Problems solved by technology

However, the traditional separation and detection method of phosphate-solubilizing bacteria has a long culture period, incomplete separation, and inconspicuous screening markers, which often lead to misjudgment or omission of the target strain of phosphate-solubilizing bacteria.

Method used

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Embodiment Construction

[0010] Below in conjunction with example the present invention will be further described, but the present invention is not limited in any way.

[0011] 1. Primary screening of phosphate-solubilizing bacteria in plant rhizosphere soil:

[0012] (1) Preparation of separation medium:

[0013] The formula is glucose 9-10 g / L, Ca 3 (PO 4 ) 2 4-5g / L, (NH 4 ) 2 SO 4 0.5 g / L, NaCl 0.2 g / L, MgSO 4 0.1 g / L, KCl 0.2 g / L, yeast extract 0.1-0.2 g / L, bromophenol blue with a mass concentration of 0.4% and pH 6.7 6mL / L, and at the same time, add the prepared soil extract with a mass concentration of 10% ml / L, agar powder 15 g / L, medium pH 7.0~7.2, sterilize the prepared culture at 115°C for 20-30 minutes, and pour the plate;

[0014] (2) Soil sample dilution and cultivation:

[0015] The soil sample comes from the rhizosphere soil of the plant. Weigh 25g of the soil sample and suspend and mix it in 225ml sterile saline to make a 1:10 sample homogeneous solution. Use a 1ml sterile ...

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Abstract

The invention provides a separation identification and phosphorus solubilizing capacity measurement method for improved phosphate solubilizing bacteria. The method comprises the following step: getting a soil sample, performing preliminary screening and secondary screening on the oil sample, so as to screen out phosphate solubilizing bacteria, and measuring the phosphorus solubilizing capacity, wherein the diameter of a phosphorus solubilizing ring and phosphorus content in a culture solution comprehensively reflect the phosphorus solubilizing capacity of strains. According to the invention, a bromophenol blue indicator is used as a selection marker, through the addition of bromophenol blue used as the indicator for the preliminary screening of the phosphate solubilizing bacteria, and observation of the phosphorus solubilizing ring and secondary screening, target strains can be screened out effectively, rapidly and visually, in addition, through the measurement of phosphorus content in the liquid culture solution, quantification to the decomposition capacity of the phosphate solubilizing bacteria can be achieved.

Description

technical field [0001] The invention belongs to the technical field of selective isolation and cultivation of phosphate-solubilizing bacteria and detection of phosphorus-solubilizing ability. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth and development. Phosphorus deficiency will affect plant growth, yield and quality. Soil is the main source of plant phosphorus. Most of the soil in our country is in a state of phosphorus deficiency. Among phosphates such as aluminum, aluminum, or complex organic phosphorus compounds, the available phosphorus content that can be absorbed and utilized by plants is generally less than 5% of the total phosphorus in the soil. In agricultural production, the application of phosphorus fertilizers is necessary, but due to the limited and non-renewable phosphate rock resources, as well as the soil compaction caused by long-term application of chemical fertilizers, people began to pay attention to the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04
Inventor 薛红芬陈云娇张晓龙罗华元董石飞周芳芳周丽娟王娟
Owner YUNNAN REASCEND TOBACCO TECH GRP
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