Qualitative detection method capable of distinguishing cow milk doped in human milk

Abstract

The invention relates to a qualitative detection method capable of distinguishing cow milk doped in human milk. The qualitative detection method is a protein gel electrophoresis method. The electrophoresis method comprises the following steps of: putting a prepared gel plate into an electrophoresis slot, adding an electrode buffer solution, performing pre-electrophoresis under 30 to 120 volts for 15 to 60 minutes, injecting the treated test sample solution into a gel sample application hole, performing electrophoresis under 50 to 200 volts until a bromophenol blue indicator is electrophoresized to the bottom of the gel, and finally taking out the gel and fixing in a fixing solution, dyeing in a dyeing solution and decoloring in a decoloring solution so as to obtain a protein electrophoresis pattern; and comparing the sample electrophoresis pattern with a characteristic protein band in the electrophoresis pattern of pure human milk and pure cow milk prepared under the same condition so as to determine whether liquid cow milk or milk powder reconstituted milk is doped into the sample milk. In the method, only one gel electrophoresis apparatus and a conventional chemical reagent are needed, the operation is simple and the result is reliable; and the cow milk or a product of the cow milk doped into the human milk can be detected.

Description

1. Technical Field [0001] The invention relates to a method for detecting the purity of substances, in particular to a qualitative detection method for identifying liquid milk or milk powder reconstituted milk mixed in human milk. 2. Background technology [0002] Foreign countries use breast milk banks to solve the problem of off-site adjustment and redistribution of excess milk. The industrialized processing of excess breast milk into a solid preparation that is easy to preserve and high in concentration is another path explored by Chinese domestic scholars. Regardless of the solution, the purity of the collected human milk (raw milk) is particularly important. [0003] According to actual operating conditions, one of the substances most likely to be incorporated into human milk is cow milk (including reconstituted milk powder, liquid milk or other dairy products with cow milk as the main component). At present, there is no research report on the purity detection of human milk ...

AI Technical Summary

Problems solved by technology

However, the ELISA method requires detection kits, and the quality of kits from different manufacturers varies, which brings uncertainties to the reproducibility of the detection results.

However, HPLC equipment is expensive, and it is necessary to purchase expensive protein standards, which is not conducive to the promotion in the early stage of industrialization

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