Method for simultaneously determining content of ethylene glycol phenyl ether and quaternary ammonium salt in disinfectant

Abstract

The invention belongs to a technology for simultaneously determining the content of two different effective components in a disinfectant, and concretely relates to a method for simultaneously determining the content of ethylene glycol phenyl ether and quaternary ammonium salt in the disinfectant. The determination method is characterized in that high-performance liquid chromatography is adopted to determine ethylene glycol phenyl ether in the disinfectant, ethylene glycol phenyl ether has ultraviolet absorption at 254[mu]m, reversed-phase high performance liquid chromatography (HPLC) is used to separate, qualitative analysis is carried out through using the retention time and an ultraviolet spectrogram, the peak area is used to quantify, and the mass fraction of ethylene glycol phenyl ether in the disinfectant is calculated according to a formula; and the quaternary ammonium salt and bromophenol blue and other acidic dyes form a colored salt in order to determine the content of the quaternary ammonium salt, and the colored salt can be easily extracted by a chlorine-containing organic solvent in an alkaline aqueous solution, and the combination of the colored salt is less stable than combination of sodium tetraphenylborate and the quaternary ammonium salt, so the property of the colored salt is used as the principle of the indicator sodium tetraphenylborate for titrating the quaternary ammonium salt, and the content of the quaternary ammonium salt in the disinfectant can be obtained through recording the titration amount of sodium tetraphenylborate and calculating according to a formula.

Description

technical field [0001] The invention belongs to the technology for determining the content of two different active ingredients contained in a disinfectant, in particular to a method for determining the content of ethylene glycol phenyl ether and a quaternary ammonium salt contained in a disinfectant. Background technique [0002] Ethylene glycol phenyl ether is commonly used as a fungicide, preservative, and acaricide in medicine. It is used as an insecticide component in pesticides, the preparation of phenoxyethylamine derivatives and the preparation of other pesticide chemical intermediates such as phenoxyether, etc. The chemical name is 2-phenoxyethanol, which is a colorless clear oily liquid. Its aqueous solution can kill Gram-positive and negative bacterial propagules, but it has only antibacterial effect on Mycobacterium tuberculosis, some fungi and bacterial spores. Therefore, ethylene glycol phenyl ether needs to be combined with other effective bactericidal ingred...

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Problems solved by technology

[0003] The disinfectant compounded by ethylene glycol phenyl ether and quaternary ammonium salt has no detection method in China at present, which brings difficulties to the content determination and quality assurance in the production process. Only the detection method of quaternary ammonium salt can be used to evaluate the An effective ingredient to represent the content of compound quaternary ammonium salts, which causes great difficulties in popularization

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