Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method

A metabolite and thin-layer detection technology, applied in the field of electrochemical detection, can solve problems such as influence, simultaneous measurement, and complex pretreatment

Inactive Publication Date: 2013-01-30
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above methods have been used for the detection of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in biological and pharmaceutical samples, many methods have the disadvantages of ...

Method used

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  • Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method
  • Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method

Examples

Experimental program
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Embodiment 1

[0025] A thin-layer detection cell for detecting serotonin and metabolites in a urine sample, comprising: a working electrode, a reference electrode and an auxiliary electrode, wherein: the input end of the working electrode, the reference electrode, the auxiliary electrode and the output end of the liquid chromatography The output terminals of the working electrode, the reference electrode and the auxiliary electrode are connected with the input terminal of the electrochemical system, and the output terminal of the electrochemical system is connected with the computer signal acquisition terminal; the working electrode includes: noble metal particle layer, A polybromophenol blue layer and an electrode substrate, wherein: noble metal particles are deposited on the polybromophenol blue film, and the polybromophenol blue is deposited on the electrode substrate.

[0026] Put the pretreated glassy carbon electrode into the phosphate buffer solution with pH=7.0, and carry out cyclic ...

Embodiment 2

[0032] A thin-layer detection cell for detecting serotonin and metabolites in urine samples and its preparation method. The pretreated glassy carbon electrode is placed in a phosphate buffer solution with pH=5.0, and the scan rate is 15mV / s at -0.2 Circular scanning process for 15min within the potential range of V~+1.0V;

[0033] Then put the electrode into the containing 1.0×10 -3 In the phosphate buffer solution of mol / L bromophenol blue pH=5.0, the scan rate of 15mV / s was used for 10 cycles in the potential range of 0.0V to +1.0V to obtain the polybromophenol blue modified electrode;

[0034] Place the polybromophenol blue modified electrode in a 2.5×10 -3 K in mol / L 2 PtCl 6 and 0.1mol / L KCl solution, cyclic scanning treatment was performed for 16 cycles at a scanning rate of 0.1V / s in the potential range of -0.4V to +0.3V, and a polybromophenol blue modified electrode loaded with platinum particles was obtained. Among them: the polybromophenol blue layer ...

Embodiment 3

[0038]A thin-layer detection cell for detecting serotonin and metabolites in urine samples and its preparation method. The pretreated glassy carbon electrode is placed in a phosphate buffer solution with pH=7.0, and the scan rate is 20mV / s at -0.2 Circular scanning process for 50min within the potential range of V~+2.0V;

[0039] Then put the electrode into the containing 1.0×10 -5 In a phosphate buffer solution with mol / L bromophenol blue at pH=7.0, the scan rate was 20mV / s, and the potential range of 0.0V to +1.0V was scanned for 30 cycles to obtain a polybromophenol blue modified electrode;

[0040] Place the polybromophenol blue modified electrode in a 4.5×10 -3 K in mol / L 2 PtCl 6 and 0.1mol / L KCl solution, cyclic scanning treatment was performed for 18 cycles in the potential range of -0.4V to +0.3V at a scanning rate of 0.1V / s, and a polybromophenol blue modified electrode loaded with platinum particles was obtained. Among them: the polybromophenol blue ...

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Abstract

A thin layer detection pool for detecting serotonins and metabolites in a urine sample comprises a work electrode, a reference electrode and an auxiliary electrode, wherein the input ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the output end of a liquid chromatograph; the output ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the input end of an electrochemical system; the output end of the electrochemical system is connected with a computer signal acquisition end; the work electrode comprises a precious metal particle layer, a poly(bromophenol blue) layer and an electrode matrix; precious metal particles are deposited on a poly(bromophenol blue) membrane; and poly(bromophenol blue) is deposited on the electrode matrix. The thin layer detection pool manufactured in the invention can realize the detection of concentrations of the serotonins and metabolites thereof in the urine sample through simple steps, the detection linearity range can reach 1.0*10<-9>-1.0*10<-5>mol/L, and the detection resolution is 1.0*10<-10>mol/L. The detection of the concentrations of the serotonins and the metabolites thereof in the urine sample can be realized through the simple steps.

Description

technical field [0001] The invention relates to a device in the technical field of electrochemical detection and a preparation method thereof, in particular to a thin-layer detection cell for detecting serotonin and metabolites in urine samples and a preparation method thereof. Background technique [0002] Serotonin, namely 5-hydroxytryptamine, exists in mast cells, platelets and argyrophilic cells of the intestinal mucosa. Studies have shown that inflammation of the appendix causes mast cells and basophils to degranulate, releases inflammatory mediators such as histamine and 5-hydroxytryptamine, and increases the concentration of serotonin in the blood. Serotonin is rapidly metabolized in the body under the action of monoamine oxidase to produce 5-hydroxyindoleacetic acid, which is excreted in the urine. Therefore, the determination of 5-hydroxytryptamine and its metabolite 5-hydroxyindoleacetic acid in urine samples has certain reference value for the diagnosis of append...

Claims

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Application Information

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IPC IPC(8): G01N27/28
Inventor 王丹张鹏王瑾何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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