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Method for detecting total alkaloid content of dendrobium candidum

A Dendrobium officinale and detection method technology, which is applied in the direction of measuring device, color/spectral characteristic measurement, material analysis through optical means, etc., can solve the problem that the dendrobium-type sesquiterpene alkaloids are not widely distributed and the maximum absorption wavelength is different , affect the sensitivity and accuracy and other issues, to achieve high accuracy, easy to reproduce, and good stability

Active Publication Date: 2018-10-12
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

The complexes formed by the reaction of different types of alkaloids and acid dyes have different maximum absorption wavelengths, so the selection of standard reference substances will greatly affect the sensitivity and accuracy of the detection results of alkaloids and acid dyes
Due to the lack of research on the chemical structure of Dendrobium officinale alkaloids, the current detection of total alkaloids in Dendrobium officinale uses dendrobine as a reference substance, but the distribution of dendrobium-type sesquiterpene alkaloids in Dendrobium is not widely distributed. The literature has confirmed that Dendrobium candidum does not contain dendrobine, therefore, it is not suitable to use dendrobine as a standard reference substance to establish the colorimetric method of total alkaloids in Dendrobium candidum

Method used

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  • Method for detecting total alkaloid content of dendrobium candidum
  • Method for detecting total alkaloid content of dendrobium candidum
  • Method for detecting total alkaloid content of dendrobium candidum

Examples

Experimental program
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Embodiment 1

[0063] 1) Prepare the extract

[0064] Take the whole plant of Dendrobium officinale, carry out deenzyming treatment at 105°C for 30min, then dry it at 60°C and make powder to obtain dry powder of Dendrobium officinale; 3 content of 25%-28%) soaked at room temperature for 40min, added 10mL of chloroform, weighed, refluxed and heated in a water bath at 80°C for 2h, added chloroform to the original weight after cooling, filtered through a 0.22 microfiltration membrane, and settled to 10mL, namely is the sample solution to be tested.

[0065] 2) Make a standard curve

[0066] 21) Take 20 mg of sanguinarine standard substance, dissolve it in appropriate amount of chloroform, and set the volume to 50 mL, which is 400 μg / mL sanguinarine mother solution, and accurately dilute according to Table 1 to obtain 40, 80, 120, 140, 160, 200 μg / mL standard solution of sanguinarine.

[0067] 22) Take 0.5mL bromophenol blue solution with a mass concentration of 0.5%, mix it with 5mL disodium...

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Abstract

The invention provides a method for detecting the total alkaloid content of dendrobium candidum. Compared with the existing dendrobium candidum acid dye colorimetric method, the method first uses sanguinarine as a standard product to replace dendrobine so that a sample to be tested has the maximum absorption wavelength (410nm) the same to that of a complex produced by the reaction of the standardproduct and an acid dye. Preferably, bromophenol blue is used as the acid dye so that the background absorption value is reduced. The novel method has the advantages of high accuracy, good stability and easy reproduction. The method provides the accurate and effective detection method for studying the accumulation rule of total alkaloids of dendrobium candidum and the quality control of the dendrobium candidum total alkaloid preparation process.

Description

technical field [0001] The invention relates to the technical field of detection of alkaloid substances, in particular to a detection method for the total alkaloid content of Dendrobium officinale. Background technique [0002] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is a perennial herb of the genus Dendrobium orchidaceae, widely distributed, and has been used in my country for thousands of years as a traditional Chinese herbal medicine and health food. It produces metabolites, which have the functions of nourishing yin and clearing heat, benefiting stomach and promoting body fluid, moistening lung and relieving cough. Because of its definite efficacy, since the 2010 edition of the Chinese Pharmacopoeia, it will be listed separately as a new species to distinguish it from the traditional Chinese medicine Dendrobium. Alkaloids are one of the important medicinal components in Dendrobium officinale, which have obvious curative effects in anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31G01N2021/3196
Inventor 魏练平郑帅韩震金青蔡永萍魏紫璇李小凡秦芸芸
Owner ANHUI AGRICULTURAL UNIVERSITY
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